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Expression Levels of ACE2 and TMPRSS2 in Different Cell Lines

Year 2023, Volume: 6 Issue: 2, 260 - 268, 30.06.2023
https://doi.org/10.53446/actamednicomedia.1253701

Abstract

Objective: ACE2 and TMPRSS2 proteins have received increased attention gained emphasis together with the pandemic COVID-19. These proteins have roles in respiratory and hypertension disorders as well as cardiovascular and renal diseases. The objective of this work was to examine the mRNA and protein levels of ACE2 and TMPRSS2 in cell lines derived from various tissue origins.
Methods: After the growth of 14 different cell lines, protein and mRNA were isolated from the cell pellets. The amounts of mRNAs and proteins were then determined and quantified using RT-PCR and ELISA.
Results: Findings showed that VERO, HEK293T, and VERO E6 cell lines significantly differed from others in the mRNA levels of both the ACE2 and TMPRSS2 genes. In protein levels obtained using ELISA, PNT1A cell line had the highest level of ACE2 protein expression, while for TMPRSS2, A549 had the highest level of protein expression.
Conclusion: It was showed in this study how the expressions of ACE2 and TMPRSS2 depend on the cell type. This may be an explanation for why virulence entrance differs in different types of tissues. It is thought that HEK293T cells with high levels of both genes may be a suitable option for studies at the RNA level by using these two genes. MCF7 may be a good option for studies at the protein level. Given the high levels of mRNA expression of both genes, it may be inferred that cells derived from the kidney were among those that were most susceptible to virus entry.

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ACE2 ve TMPRSS2 Genlerinin Farklı Hücre Hatlarındaki İfade Düzeyleri

Year 2023, Volume: 6 Issue: 2, 260 - 268, 30.06.2023
https://doi.org/10.53446/actamednicomedia.1253701

Abstract

Amaç: ACE2 ve TMPRSS2 proteinleri, COVID-19 pandemisi ile birlikte önem kazanmıştır. Bu proteinlerin solunum ve hipertansiyon bozukluklarının yanı sıra kardiyovasküler ve renal hastalıklarda rolleri vardır. Bu çalışmanın amacı, çeşitli dokulardan üretilen hücre hatlarında ACE2 ve TMPRSS2'nin mRNA ve protein seviyelerini belirlemektir.
Yöntem: 14 hücre hattı kültürde çoğaltıldıktan sonra, hücre pelletlerinden protein ve mRNA izole edildi. Ardından mRNA seviyeleri RT-PCR ve protein seviyeleri ise ELISA metotları kullanılarak ölçüldü.
Bulgular: Çalışmanın sonuçları, ACE2 ve TMPRSS2 genlerinin mRNA seviyelerinin VERO, HEK293T ve VERO E6 hücre hatlarında, diğer hücre hatlarına kıyasla, önemli ölçüde yüksek olduğunu göstermiştir. ELISA kullanılarak elde edilen protein seviyelerinde, PNT1A hücre hattı en yüksek ACE2 protein ekspresyonuna sahipken, TMPRSS2 için A549 en yüksek protein ekspresyonuna sahip olarak bulunmuştur.
Sonuç: Bu çalışmada ACE2 ve TMPRSS2 gen ifadelerinin hücre tipine göre farklılaşabileceği gösterilmiştir. Bu sonuç, farklı doku tiplerinde virüs girişinin neden farklı olduğunun bir açıklaması olabilir. Her iki genin de yüksek düzeyde bulunduğu HEK293T hücrelerinin, bu iki gen kullanılarak RNA düzeyinde yapılacak çalışmalar için uygun bir seçenek olabileceği düşünülmektedir. MCF7 hücre hattı ise protein seviyesindeki çalışmalar için iyi bir seçenek olabilir. Her iki genin yüksek mRNA ekspresyon seviyeleri göz önüne alındığında, böbrekten üretilen hücre hatlarının virüs girişine en duyarlı olanlar arasında olduğu sonucuna varılabilir.

References

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  • 22. Mohamed MS, Moulin TC, Schiöth HB. Sex differences in COVID-19: the role of androgens in disease severity and progression. Endocrine. 2021;71(1):3-8. doi:10.1007/s12020-020-02536-6
  • 23. Shen LW, Mao HJ, Wu YL, Tanaka Y, Zhang W. TMPRSS2: A potential target for treatment of influenza virus and coronavirus infections. Biochimie. 2017;142:1-10. doi:10.1016/j.biochi.2017.07.016
  • 24. Singh H, Choudhari R, Nema V, Khan AA. ACE2 and TMPRSS2 polymorphisms in various diseases with special reference to its impact on COVID-19 disease. Microb Pathogenesis. 2021;150:104621-104621. doi:10.1016/j.micpath.2020.104621
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  • 26. Afar DE, Vivanco I, Hubert RS, et al. Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epithelia. Cancer Res. 2001;61(4):1686-1692.
  • 27. Jacquinet E, Rao NV, Rao GV, Hoidal JR. Cloning, genomic organization, chromosomal assignment and expression of a novel mosaic serine proteinase: epitheliasin. Febs Lett. 2000;468(1):93-100. doi:10.1016/s0014-5793(00)01196-0
  • 28. Zhang H, Rostami MR, Leopold PL, et al. Expression of the SARS-CoV-2 ACE2 Receptor in the Human Airway Epithelium. Am J Resp Crit Care. 2020;202(2):219-229. doi:10.1164/rccm.202003-0541oc
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There are 57 citations in total.

Details

Primary Language English
Subjects Clinical Sciences (Other)
Journal Section Research Articles
Authors

Merve Gülsen Bal Albayrak 0000-0003-2444-4258

Sevinc Yanar 0000-0002-6438-7385

Murat Kasap 0000-0001-8527-2096

Gürler Akpınar 0000-0002-9675-3714

Publication Date June 30, 2023
Submission Date February 20, 2023
Acceptance Date May 10, 2023
Published in Issue Year 2023 Volume: 6 Issue: 2

Cite

AMA Bal Albayrak MG, Yanar S, Kasap M, Akpınar G. Expression Levels of ACE2 and TMPRSS2 in Different Cell Lines. Acta Med Nicomedia. June 2023;6(2):260-268. doi:10.53446/actamednicomedia.1253701

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