Research Article
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Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation

Year 2020, Volume: 1 Issue: 1, 1 - 7, 19.06.2020

Abstract

Until today, several disinfecting methods have been proposed and studied for cleaning and disinfection, containing heavy metals and chlorine, to reduce the biological load on environmental surfaces. Most of the proposed techniques are based on the use of chemical compounds. These methods have proven to be effective in reducing the majority of pathogens, but have been ineffective in preventing the persistence of pollutant microorganisms. Nowadays, increasing demand for natural and green products has led to recognition of cleaning products containing microbial based cultures. Cleaning products containing live microorganisms as active substances are becoming increasingly common in homes and industrial cleaning applications. These products are called "probiotic or microbial" cleaners. Microbial based cleaning products are environmentally cleaning products that contain bacteria or spores that are suitable as active ingredients. The purpose of using these cleaning systems is to prevent the development of pathogens by tolerating the presence of harmless probiotic microorganisms on surfaces. The aim of this study is to investigate the viability of B. subtilis species at different pH values for use in detergent formulation and the effective removal of pathogenic bacteria and fungi on the surface in the long term. As a result, it was observed that the probiotic B.subtilis, which can be used in the microbial based cleaner formulation, showed higher growth and vitality at the neutral pH and exhibited high antibiotic resistance, auto-aggregation, antagonistic and antifungal ability. Based on these findings, B. subtilis can be used to prevent the development of pathogenic species.

Supporting Institution

TUBITAK 2244

Project Number

118C075

References

  • Adimpong DB, Sørensen KI, Thorsen L, Stuer-Lauridsen B, Abdelgadir WS, Nielsen DS, Jespersen L (2012) Antimicrobial susceptibility of Bacillus strains isolated from primary starters for African traditional bread production and characterization of the bacitracin operon and bacitracin biosynthesis. Appl Environ Microbiol 78(22): 7903-7914
  • Aher NG, Pore VS, Mishra NN, Kumar A, Shukla PK, Sharma A, Bhat M K (2009) Synthesis and antifungal activity of 1, 2, 3-triazole containing fluconazole analogues. Bioorg Med Chem Lett 19(3): 759-763
  • Ahmed S, Mustafa G, Arshad M, Rajoka MI (2017) Fungal Biomass Protein Production from. BioMed Res Int 6232793: 9
  • Arcales JAA, Alolod GAL (2018) Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus). Curr Res Nutr Food Sci J 6(2): 500-508
  • Baranyi J, Pin C (1999) Estimating Bacterial Growth Parameters by Means of Detection Times. Appl Environ Microbiol 65(2): 732–736
  • Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496
  • Bauzad M, Yuliati FN, Prahesti KI, Malaka R (2019) Total plate count and Escherichia coli in raw buffalo milk in curio district enrekang regency. In IOP Conference Series: Earth Environ Sci 247(1): 012027.
  • Bhatta H, Goldys E M, Learmonth R P (2006) Use of fluorescence spectroscopy to differentiate yeast and bacterial cells. Appl Microbiol Biotechnol 71(1): 121-126
  • Brooke A, Selby GB (2014) Cleaning compositions. US Patent 8,801, 864 B2, 2014
  • Caselli E (2017) Hygiene: microbial strategies to reduce pathogens and drug resistance in clinical settings. Microb Biotechnol 10(5): 1079–1083
  • Caselli E, Arnoldo L, Rognoni C, D’Accolti M, Soffritti I, Lanzoni L, Bisi M, Volta A, Tarricone R, Brusaferro S, Mazzacane S (2019) Impact of a probiotic-based hospital sanitation on antimicrobial resistance and HAI-associated antimicrobial consumption and costs: a multicenter study. Infect Drug Resist 12: 501–510
  • Caselli E, D’Accolti M, Vandini A, Lanzoni L, Teresa Camerada M, Coccagna M, Branchini A, Antonioli P, Balboni PG, Di Luca D, Mazzacane S (2016) Impact of a Probiotic-Based Cleaning Intervention on the Microbiota Ecosystem of the Hospital Surfaces: Focus on the Resistome Remodulation. PLoS One,11(2)
  • Collado MC, Meriluoto J, Salminen S (2008) Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Technol 226: 1065–1073
  • D’Accolti M, Soffritti I, Piffanelli M, Bisi M, Mazzacane S, Caselli E (2018) Efficient removal of hospital pathogens from hard surfaces by a combined use of bacteriophages and probiotics: potential as sanitizing agents. Infect Drug Resist 11: 1015–1026
  • De Cesare A, Caselli E, Lucchi A, Sala C, Parisi A, Manfreda G, Mazzacane S (2019) Impact of a probiotic-based cleaning product on the microbiological profile of broiler litters and chicken caeca microbiota. Poult Sci 98(9): 3602-3610
  • Doĝanay M, Aydin N (1991) Antimicrobial susceptibility of Bacillus anthracis. Scand J Infect Dis 23(3): 333-335
  • Faille C, Membre J M, Kubaczka M, Gavini F (2002) Altered ability of Bacillus cereus spores to grow under unfavorable conditions (presence of nisin, low temperature, acidic pH, presence of NaCl) following heat treatment during sporulation. J Food Prot 65(12): 1930-1936
  • Gómez NC, Ramiro JM, Quecan BX, de Melo Franco BD (2016) Use of Potential Probiotic Lactic Acid Bacteria (LAB) Biofilms for the Control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7 Biofilms Formation. Front Microbiol 7, 863
  • Hachmann A B, Angert ER, Helmann JD (2009) Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin. Antimicrob Agents Chemother 53(4): 1598-1609
  • Hong HA, Huang JM, Khaneja R, Hiep LV, Urdaci MC, Cutting SM (2008) The safety of Bacillus subtilis and Bacillus indicus as food probiotics. J Appl Microbiol 105(2), 510-520
  • Lee NK, Kim SY, Choi SY, Paik HD (2013) Probiotic Bacillus subtilis KU201 having antifungal and antimicrobial properties isolated from kimchi. Food Sci Biotechnol 22(5), 1-5
  • Mazza P, Zani F, Martelli P (1992) Studies on the antibiotic resistance of Bacillus subtilis strains used in oral bacteriotherapy. Boll Chim Farm 131:401–408
  • Moore T, Globa L, Barbaree L, Vodyanoy V, Sorokulova I (2013) Antagonistic activity of Bacillus Bacteria against Food-Borne Pathogens. J Probiotics Health, 1: 3
  • Özçimen D (2018) Investigation of Antifungal Effect of Chlorella Protothecoides Microalgae Oil Against Botrytis cinerea and Aspergillus niger fungi. J Tekirdag Agric Fac, 15-2, 45-52 Ritter AC, Paula A, Correa F, Veras FF, Brandelli A (2018) Characterization of Bacillus subtilis available as probiotics. J Microbiol Res 8(2): 23-32
  • Spöka A, Arvanitakisb G, McClung G (2018) Status of microbial based cleaning products in statutory regulations and ecolabelling in Europe, the USA, and Canada. Food Chem Toxicol 116: 10–19
  • Teasdale SM, Kademi A (2018) Quality challenges associated with microbial-based cleaning products from the Industry Perspective. Food Chem Toxicol 116: 20–24
  • Vandini A, Frabetti A, Antonioli P, Platano D, Branchini A, Teresa Camerada M, Lanzoni L, Balboni P, Mazzacane S (2014) Reduction of the Microbiological Load on Hospital Surfaces Through Probiotic-Based Cleaning Procedures: A New Strategy to Control Nosocomial Infections. J Microbiol Exp 1(5)
  • Vehapi M, Yilmaz A, Özçimen D (2018) Antifungal activities of Chlorella vulgaris and Chlorella minutissima microalgae cultivated in Bold Basal Medium, Wastewater and Extract Water against Aspergillus niger and Fusarium oxysporum. Rom Biotechnol Lett, doi: 10.26327/RBL2018.228
  • Wang H, Wang F, Wang W, Yao X, Wei D, Cheng H, Deng Z (2014) Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. PloS one 9(11)
  • Weber DJ, Saviteer SM, Rutala WA, Thomann CA (1988) In vitro susceptibility of Bacillus spp. to selected antimicrobial agents. Antimicrob Agents Chemother 32(5): 642-645
  • Winslow CE, Walker HH (1939) The earlier phases of the bacterial culture cycle. Bacteriol Rev 3(2): 147
  • Wilks J C, Kitko RD, Cleeton SH, Lee GE, Ugwu CS, Jones BD, ... & Slonczewski J L (2009) Acid and base stress and transcriptomic responses in Bacillus subtilis. Appl Environ Microbiol 75(4): 981-990
  • Woo J, Ahn J (2013) Probiotic‐mediated competition, exclusion and displacement in biofilm formation by food‐borne pathogens. Lett Appl Microbiol 56(4): 307-313
Year 2020, Volume: 1 Issue: 1, 1 - 7, 19.06.2020

Abstract

Project Number

118C075

References

  • Adimpong DB, Sørensen KI, Thorsen L, Stuer-Lauridsen B, Abdelgadir WS, Nielsen DS, Jespersen L (2012) Antimicrobial susceptibility of Bacillus strains isolated from primary starters for African traditional bread production and characterization of the bacitracin operon and bacitracin biosynthesis. Appl Environ Microbiol 78(22): 7903-7914
  • Aher NG, Pore VS, Mishra NN, Kumar A, Shukla PK, Sharma A, Bhat M K (2009) Synthesis and antifungal activity of 1, 2, 3-triazole containing fluconazole analogues. Bioorg Med Chem Lett 19(3): 759-763
  • Ahmed S, Mustafa G, Arshad M, Rajoka MI (2017) Fungal Biomass Protein Production from. BioMed Res Int 6232793: 9
  • Arcales JAA, Alolod GAL (2018) Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus). Curr Res Nutr Food Sci J 6(2): 500-508
  • Baranyi J, Pin C (1999) Estimating Bacterial Growth Parameters by Means of Detection Times. Appl Environ Microbiol 65(2): 732–736
  • Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493–496
  • Bauzad M, Yuliati FN, Prahesti KI, Malaka R (2019) Total plate count and Escherichia coli in raw buffalo milk in curio district enrekang regency. In IOP Conference Series: Earth Environ Sci 247(1): 012027.
  • Bhatta H, Goldys E M, Learmonth R P (2006) Use of fluorescence spectroscopy to differentiate yeast and bacterial cells. Appl Microbiol Biotechnol 71(1): 121-126
  • Brooke A, Selby GB (2014) Cleaning compositions. US Patent 8,801, 864 B2, 2014
  • Caselli E (2017) Hygiene: microbial strategies to reduce pathogens and drug resistance in clinical settings. Microb Biotechnol 10(5): 1079–1083
  • Caselli E, Arnoldo L, Rognoni C, D’Accolti M, Soffritti I, Lanzoni L, Bisi M, Volta A, Tarricone R, Brusaferro S, Mazzacane S (2019) Impact of a probiotic-based hospital sanitation on antimicrobial resistance and HAI-associated antimicrobial consumption and costs: a multicenter study. Infect Drug Resist 12: 501–510
  • Caselli E, D’Accolti M, Vandini A, Lanzoni L, Teresa Camerada M, Coccagna M, Branchini A, Antonioli P, Balboni PG, Di Luca D, Mazzacane S (2016) Impact of a Probiotic-Based Cleaning Intervention on the Microbiota Ecosystem of the Hospital Surfaces: Focus on the Resistome Remodulation. PLoS One,11(2)
  • Collado MC, Meriluoto J, Salminen S (2008) Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Technol 226: 1065–1073
  • D’Accolti M, Soffritti I, Piffanelli M, Bisi M, Mazzacane S, Caselli E (2018) Efficient removal of hospital pathogens from hard surfaces by a combined use of bacteriophages and probiotics: potential as sanitizing agents. Infect Drug Resist 11: 1015–1026
  • De Cesare A, Caselli E, Lucchi A, Sala C, Parisi A, Manfreda G, Mazzacane S (2019) Impact of a probiotic-based cleaning product on the microbiological profile of broiler litters and chicken caeca microbiota. Poult Sci 98(9): 3602-3610
  • Doĝanay M, Aydin N (1991) Antimicrobial susceptibility of Bacillus anthracis. Scand J Infect Dis 23(3): 333-335
  • Faille C, Membre J M, Kubaczka M, Gavini F (2002) Altered ability of Bacillus cereus spores to grow under unfavorable conditions (presence of nisin, low temperature, acidic pH, presence of NaCl) following heat treatment during sporulation. J Food Prot 65(12): 1930-1936
  • Gómez NC, Ramiro JM, Quecan BX, de Melo Franco BD (2016) Use of Potential Probiotic Lactic Acid Bacteria (LAB) Biofilms for the Control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157:H7 Biofilms Formation. Front Microbiol 7, 863
  • Hachmann A B, Angert ER, Helmann JD (2009) Genetic analysis of factors affecting susceptibility of Bacillus subtilis to daptomycin. Antimicrob Agents Chemother 53(4): 1598-1609
  • Hong HA, Huang JM, Khaneja R, Hiep LV, Urdaci MC, Cutting SM (2008) The safety of Bacillus subtilis and Bacillus indicus as food probiotics. J Appl Microbiol 105(2), 510-520
  • Lee NK, Kim SY, Choi SY, Paik HD (2013) Probiotic Bacillus subtilis KU201 having antifungal and antimicrobial properties isolated from kimchi. Food Sci Biotechnol 22(5), 1-5
  • Mazza P, Zani F, Martelli P (1992) Studies on the antibiotic resistance of Bacillus subtilis strains used in oral bacteriotherapy. Boll Chim Farm 131:401–408
  • Moore T, Globa L, Barbaree L, Vodyanoy V, Sorokulova I (2013) Antagonistic activity of Bacillus Bacteria against Food-Borne Pathogens. J Probiotics Health, 1: 3
  • Özçimen D (2018) Investigation of Antifungal Effect of Chlorella Protothecoides Microalgae Oil Against Botrytis cinerea and Aspergillus niger fungi. J Tekirdag Agric Fac, 15-2, 45-52 Ritter AC, Paula A, Correa F, Veras FF, Brandelli A (2018) Characterization of Bacillus subtilis available as probiotics. J Microbiol Res 8(2): 23-32
  • Spöka A, Arvanitakisb G, McClung G (2018) Status of microbial based cleaning products in statutory regulations and ecolabelling in Europe, the USA, and Canada. Food Chem Toxicol 116: 10–19
  • Teasdale SM, Kademi A (2018) Quality challenges associated with microbial-based cleaning products from the Industry Perspective. Food Chem Toxicol 116: 20–24
  • Vandini A, Frabetti A, Antonioli P, Platano D, Branchini A, Teresa Camerada M, Lanzoni L, Balboni P, Mazzacane S (2014) Reduction of the Microbiological Load on Hospital Surfaces Through Probiotic-Based Cleaning Procedures: A New Strategy to Control Nosocomial Infections. J Microbiol Exp 1(5)
  • Vehapi M, Yilmaz A, Özçimen D (2018) Antifungal activities of Chlorella vulgaris and Chlorella minutissima microalgae cultivated in Bold Basal Medium, Wastewater and Extract Water against Aspergillus niger and Fusarium oxysporum. Rom Biotechnol Lett, doi: 10.26327/RBL2018.228
  • Wang H, Wang F, Wang W, Yao X, Wei D, Cheng H, Deng Z (2014) Improving the expression of recombinant proteins in E. coli BL21 (DE3) under acetate stress: an alkaline pH shift approach. PloS one 9(11)
  • Weber DJ, Saviteer SM, Rutala WA, Thomann CA (1988) In vitro susceptibility of Bacillus spp. to selected antimicrobial agents. Antimicrob Agents Chemother 32(5): 642-645
  • Winslow CE, Walker HH (1939) The earlier phases of the bacterial culture cycle. Bacteriol Rev 3(2): 147
  • Wilks J C, Kitko RD, Cleeton SH, Lee GE, Ugwu CS, Jones BD, ... & Slonczewski J L (2009) Acid and base stress and transcriptomic responses in Bacillus subtilis. Appl Environ Microbiol 75(4): 981-990
  • Woo J, Ahn J (2013) Probiotic‐mediated competition, exclusion and displacement in biofilm formation by food‐borne pathogens. Lett Appl Microbiol 56(4): 307-313
There are 33 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Articles
Authors

Meyrem Vehapi This is me

Didem Özçimen

Project Number 118C075
Publication Date June 19, 2020
Acceptance Date May 22, 2020
Published in Issue Year 2020 Volume: 1 Issue: 1

Cite

APA Vehapi, M., & Özçimen, D. (2020). Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation. Bulletin of Biotechnology, 1(1), 1-7.
AMA Vehapi M, Özçimen D. Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation. Bull. Biotechnol. June 2020;1(1):1-7.
Chicago Vehapi, Meyrem, and Didem Özçimen. “Investigation of B. Subtilis Viability at Different PH Ranges for Use in Microbial Cleaner Formulation”. Bulletin of Biotechnology 1, no. 1 (June 2020): 1-7.
EndNote Vehapi M, Özçimen D (June 1, 2020) Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation. Bulletin of Biotechnology 1 1 1–7.
IEEE M. Vehapi and D. Özçimen, “Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation”, Bull. Biotechnol., vol. 1, no. 1, pp. 1–7, 2020.
ISNAD Vehapi, Meyrem - Özçimen, Didem. “Investigation of B. Subtilis Viability at Different PH Ranges for Use in Microbial Cleaner Formulation”. Bulletin of Biotechnology 1/1 (June 2020), 1-7.
JAMA Vehapi M, Özçimen D. Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation. Bull. Biotechnol. 2020;1:1–7.
MLA Vehapi, Meyrem and Didem Özçimen. “Investigation of B. Subtilis Viability at Different PH Ranges for Use in Microbial Cleaner Formulation”. Bulletin of Biotechnology, vol. 1, no. 1, 2020, pp. 1-7.
Vancouver Vehapi M, Özçimen D. Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation. Bull. Biotechnol. 2020;1(1):1-7.