Research Article
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An evergreen host for Cucumber Mosaic Virus (CMV) in Turkey: Polygala myrtifolia

Year 2017, Volume: 34 Issue: 1, 29 - 36, 01.06.2017
https://doi.org/10.16882/derim.2017.305281

Abstract

Polygala myrtifolia is
known as Myrtle-leaf milkwort or Petite Butterflys, which is cultivated as an
ornamental in Turkey. During the observations in 2014-2015 summers, systemic
mosaic and irregular yellowish lines were recorded on leaves. In order to
identify causal agent, leaf samples were tested by DAS-ELISA using specific
polyclonal antiserums against to TRSV (Tobacco
Ringspot Virus
), ToRSV (Tomato
Ringspot Virus
), SLRSV (Strawberry
Latent Ringspot Virus
), ArMV (Arabis
Mosaic Virus
), CMV (Cucumber Mosaic
Virus
), TMV (Tobacco Mosaic Virus),
TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), Tospo (Tospovirus), TSWV (Tomato Spotted Wilt Virus), PVX (Potato Virus X), SqMV (Squash
Mosaic Virus
), Poty (Group Poty Virus),
PVY (Potato Virus Y), AMV (Alfalfa Mosaic Virus) and ZYMV (Zucchini Yellow Mosaic Virus), supplied
by BIOREBA AG for common ornamental plant viruses. The samples (8 individual,
13.1%) which are positive for CMV in DAS-ELISA, were confirmed by RT-PCR using
total RNA extracted from leaves (100 mg). 513 bp cDNA bands in RT-PCR assay by
primer pairs Sense CMVr and Antisense CMVf were observed in 1.5% agarose gel.
In Turkey, this study is the first record of CMV via serologicaly and moleculer
technics on Polygala that is evergreen in Antalya, may be also evidence of
uncontrolled distribution between species and vectors.

References

  • Anonymous, (2016). Polygala. https://en.wikipedia. org/wiki/Polygala. Erişim tarihi: 27 Nisan 2016.
  • Arneodo, J.D., Guzman, F.A., Nome, C.F., & Conci, L.R. (2005). Occurrence of cucumber mosaic virus on Antirrhinum msjus in Argentina. Fitopatologia Brasileira, 30(1):90.
  • Baumgartnerova, H. (1994). Rosa sp. – reservoir of the sour cherry necrotic ringspot virus. Acta Horticulturae, 377:357-59.
  • Bellardi, M.G., Nanni, G., & Bertaccini, A. (2002). Old and new viruses of lily in Italy. Acta Horticulturae, 568:215-220.
  • Bertolini, E., Olmos, A., Martı́nez, M.C., Gorris, M.T., & Cambra, M. (2001). Single-step multiplex RT-PCR for simultaneous and colourimetric detection of six RNA viruses in olive trees. Journal of Virological Methods, 96(1):33-41.
  • Blancard, D. (1988). Maladies de la tomate. Observer, identifier, lutter. Paris, FRA:INRA Editions, 212 p.
  • Buzkan, N., & Yüzer, D. (2009). Molecular detection of seed-borne viruses in Kahramanmaraş red peppers. Alatarım, 8(1):1-7.
  • Çağlar, B.K. (2006). Hıyar Mozaik Virüsü (CMV)’nün kavun (CMV-K), domates (CMV-D), biber (CMV-B) izolatlarının biyolojik, serolojik, moleküler yöntemlerle karakterizasyonu ve Satellit RNA’lerin virüs üzerindeki etkisi. Doktora Tezi, Çukurova Üniversitesi, Adana.
  • Cardin, L., & Onesto, J.P. (2005). Mosaic symptoms induced by Cucumber mosaic virus in Polygala myrtifolia in France and New Zealand. Plant Disease, 89(5):527.
  • Clark, M.F., & Adams, A.N. (1977). Characteristics of the micro-plate method of enzime linked immuno sorbent assay for the detection of plant viruses. Journal of General Virology, 34(3):475-483.
  • Chen, Y.K., Chen, C.C., Ko, W.F., & Chen, M.J. (1995a). Isolation and identification of cucumber mosaic virus from larkspur. Plant Protection Bulletin-Taipei, 37(3):319-330.
  • Chen, Y.K., Derks, A.M., Langeveld, S.A., Goldbach, R., & Pris, M. (2001). High sequence conservation among cucumber mosaic virus isolated from Lily. Archives of Virology, 146(8):1631-1636.
  • Chen, Y.K., Lin, W.F., & Chen, M.J. (1995b). Amaranthus rugose-a disease caused by cucumber mosaic virus (CMV). Plant Protection Bulletin-Taipei, 37(2):97-106.
  • Chen, Y.K., Prins, M., Derks, A.M., Langeveld, S.A., & Goldbach, R. (2002). Alstroemeria-infecting cucumber mosaic virus isolates contain additional sequences in the RNA3 segment. Acta Horticulturae, 568:51-57.
  • Gallitelli, D. (2000). The ecology of cucumber mosaic virus and sustainable agriculture. Virus Research, 71(1-2):9-12.
  • Hsu, H.T., Barzuna, L., Hsu, Y. H., Bliss, W., & Perry, K.L., 2000. Identification and subgrouping of cucumber mosaic virus with mouse monoclonal antibodies. Phytopathology, 90(6):615-620
  • Hsu, H.T. (2002). Biological Control of Plant Pathogens (Viruses). In: David P, Editor. Encyclopedia of Pest Management. New York, Marcel Dekker, p 68.
  • Katoch, M., Abodin, M.Z., Raja, R., & Zaidi, A.A. (2003). An overview of diagnostics for viruses infecting gladiolus. Crop Protection, 22(1):153-156.
  • Kearney, C.M., Gonsalves, D., & Provvidenti, R. (1990). A severe strain of cucumber mosaic virus from China and associated satellite RNA. Plant Disease, 74(10):819-823.
  • Okuno, K., Komeya-Iwaki, M., Takeshita, M., Furuya, N., & Takanami, Y. (2002). A cucumber mosaic virus isolated from Silene armeria. Journal of General Plant Pathology, 68(1):108-109.
  • Palukaitis, P., Roossinck, M.J., Dietzgen, R.G., & Francki, R.I.B. (1992). Cucumber mosaic virus. Advance Virus Research, 41:281-348.
  • Rybicki, E.P. (1995). The Bromoviridae p. In: Murbhy A, Fouquet CM, Bishop DHL, Ghabrial SAG, Jarvis AW, Martelli GP, Mayo MA, Summers MD. Virus Taxonomy: 6th Report of the International Committee on Taxonomy of Viruses. Springer-Verlag Wien, New York, USA. pp. 450-457.
  • Raj, S.K., Srivastava, A., Chandra, G., & Singh, B.P. (2002). Characterizations of cucumbe rmosaic virus isolate infecting gladiolus cultivars and comparative evaluation of serological and molecular methods for sensitive diagnosis. Current Science, 83(9):1132-1137.
  • Rist, D.I., & Lorbeer, J.W. (1991). Relationships of weed reservoirs to cucumber mosaic virus (CMV) and broad bean wilt virus (BBWV) in commercial lettuce fields in New York. Phytopathology, 81(4):367-371.
  • Sayama, H., Kominato, M., Atarashi, H., Takayanagi, N., Yamada, M., Hikage, T., & Yoshike, T. (2006). Control of cucumber mosaic virus (CMV) in gentian by satellite RNA containing attenuated CMV strains. Acta Horticulturea, 722:147-154.
  • Takami, K., Okubo, H., Yamasaki, S., Takeshita, M., & Takanami, Y. (2006). A cucumber mosaicvirus isolated from Momordica charantia L. Journal of General Plant Pathology, 72(6):391-392.
  • Tessitori, M., Reina, A., Catara, V., & Polizzi, G. (2002). Polygala myrtifolia as a new natural host of Cucumber mosaic virus. Plant Disease, 86(12):1403.
  • Verma, N., Mahinghara, B.K., Ram, R., & Zaidi, A.A. (2006). Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II. Journal of Biosciences, 31(1):47-54.

Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia

Year 2017, Volume: 34 Issue: 1, 29 - 36, 01.06.2017
https://doi.org/10.16882/derim.2017.305281

Abstract

Mersin Yapraklı Sütotu ya da Küçük Kelebek, olarak
bilinen Polygala myrtifolia,
Türkiye’de süs bitkisi olarak yetiştirilmektedir. 2014-2015 yaz mevsimlerinde
yapılan gözlemler sırasında Mersin Yapraklı Sütotu bitkilerinin yapraklarında,
sistemik mozaik ve düzensiz sarımsı izler gözlenmiştir. Neden olan etmenin
tanılanma için yaprak örnekleri BIOREBA AG’dan (Switzerland) temin edilen
spesifik poliklonal antiserum ile DAS-ELISA tekniğine göre TRSV (Tobacco Ringspot Virus), ToRSV (Tomato Ringspot Virus), SLRSV (Strawberry Latent Ringspot Virus), ArMV
(Arabis Mosaic Virus), CMV (Cucumber Mosaic Virus), TMV (Tobacco Mosaic Virus), TNV (Tobacco Necrosis Virus), ToMV (Tomato Mosaic Virus), Tospo (Tospovirus), TSWV (Tomato Spotted Wilt Virus), PVX (Potato Virus X), SqMV (Squash
Mosaic Virus
), Poty (Group Poty Virus),
PVY (Potato Virus Y), AMV (Alfalfa Mosaic Virus) ve ZYMV (Zucchini Yellow Mosaic Virus) gibi
bilinen süs bitkisi virüslerine karşı testlenmiştir. DAS-ELISA’da CMV pozitif
örnek (8 adet, %13.1) sonuçları, 100 mg yapraktan ekstrakte edilen toplam
RNA’ların RT-PCR’da kullanılmasıyla doğrulanmıştır. Sense CMVr ve Anti sense
CMVf primerleriyle yapılan RT-PCR sonucu, 513 bp cDNA bandları %1.5’lik agaroz
jelde gözlenmiştir. Antalya’da her dem yeşil olan Sütotu’nda CMV’nin serolojik
ve moleküler tekniklerle ilk kaydı olan bu çalışma belki de türler ve bölgeler
arasında kontrolsüz yayılımının kanıtıdır.

References

  • Anonymous, (2016). Polygala. https://en.wikipedia. org/wiki/Polygala. Erişim tarihi: 27 Nisan 2016.
  • Arneodo, J.D., Guzman, F.A., Nome, C.F., & Conci, L.R. (2005). Occurrence of cucumber mosaic virus on Antirrhinum msjus in Argentina. Fitopatologia Brasileira, 30(1):90.
  • Baumgartnerova, H. (1994). Rosa sp. – reservoir of the sour cherry necrotic ringspot virus. Acta Horticulturae, 377:357-59.
  • Bellardi, M.G., Nanni, G., & Bertaccini, A. (2002). Old and new viruses of lily in Italy. Acta Horticulturae, 568:215-220.
  • Bertolini, E., Olmos, A., Martı́nez, M.C., Gorris, M.T., & Cambra, M. (2001). Single-step multiplex RT-PCR for simultaneous and colourimetric detection of six RNA viruses in olive trees. Journal of Virological Methods, 96(1):33-41.
  • Blancard, D. (1988). Maladies de la tomate. Observer, identifier, lutter. Paris, FRA:INRA Editions, 212 p.
  • Buzkan, N., & Yüzer, D. (2009). Molecular detection of seed-borne viruses in Kahramanmaraş red peppers. Alatarım, 8(1):1-7.
  • Çağlar, B.K. (2006). Hıyar Mozaik Virüsü (CMV)’nün kavun (CMV-K), domates (CMV-D), biber (CMV-B) izolatlarının biyolojik, serolojik, moleküler yöntemlerle karakterizasyonu ve Satellit RNA’lerin virüs üzerindeki etkisi. Doktora Tezi, Çukurova Üniversitesi, Adana.
  • Cardin, L., & Onesto, J.P. (2005). Mosaic symptoms induced by Cucumber mosaic virus in Polygala myrtifolia in France and New Zealand. Plant Disease, 89(5):527.
  • Clark, M.F., & Adams, A.N. (1977). Characteristics of the micro-plate method of enzime linked immuno sorbent assay for the detection of plant viruses. Journal of General Virology, 34(3):475-483.
  • Chen, Y.K., Chen, C.C., Ko, W.F., & Chen, M.J. (1995a). Isolation and identification of cucumber mosaic virus from larkspur. Plant Protection Bulletin-Taipei, 37(3):319-330.
  • Chen, Y.K., Derks, A.M., Langeveld, S.A., Goldbach, R., & Pris, M. (2001). High sequence conservation among cucumber mosaic virus isolated from Lily. Archives of Virology, 146(8):1631-1636.
  • Chen, Y.K., Lin, W.F., & Chen, M.J. (1995b). Amaranthus rugose-a disease caused by cucumber mosaic virus (CMV). Plant Protection Bulletin-Taipei, 37(2):97-106.
  • Chen, Y.K., Prins, M., Derks, A.M., Langeveld, S.A., & Goldbach, R. (2002). Alstroemeria-infecting cucumber mosaic virus isolates contain additional sequences in the RNA3 segment. Acta Horticulturae, 568:51-57.
  • Gallitelli, D. (2000). The ecology of cucumber mosaic virus and sustainable agriculture. Virus Research, 71(1-2):9-12.
  • Hsu, H.T., Barzuna, L., Hsu, Y. H., Bliss, W., & Perry, K.L., 2000. Identification and subgrouping of cucumber mosaic virus with mouse monoclonal antibodies. Phytopathology, 90(6):615-620
  • Hsu, H.T. (2002). Biological Control of Plant Pathogens (Viruses). In: David P, Editor. Encyclopedia of Pest Management. New York, Marcel Dekker, p 68.
  • Katoch, M., Abodin, M.Z., Raja, R., & Zaidi, A.A. (2003). An overview of diagnostics for viruses infecting gladiolus. Crop Protection, 22(1):153-156.
  • Kearney, C.M., Gonsalves, D., & Provvidenti, R. (1990). A severe strain of cucumber mosaic virus from China and associated satellite RNA. Plant Disease, 74(10):819-823.
  • Okuno, K., Komeya-Iwaki, M., Takeshita, M., Furuya, N., & Takanami, Y. (2002). A cucumber mosaic virus isolated from Silene armeria. Journal of General Plant Pathology, 68(1):108-109.
  • Palukaitis, P., Roossinck, M.J., Dietzgen, R.G., & Francki, R.I.B. (1992). Cucumber mosaic virus. Advance Virus Research, 41:281-348.
  • Rybicki, E.P. (1995). The Bromoviridae p. In: Murbhy A, Fouquet CM, Bishop DHL, Ghabrial SAG, Jarvis AW, Martelli GP, Mayo MA, Summers MD. Virus Taxonomy: 6th Report of the International Committee on Taxonomy of Viruses. Springer-Verlag Wien, New York, USA. pp. 450-457.
  • Raj, S.K., Srivastava, A., Chandra, G., & Singh, B.P. (2002). Characterizations of cucumbe rmosaic virus isolate infecting gladiolus cultivars and comparative evaluation of serological and molecular methods for sensitive diagnosis. Current Science, 83(9):1132-1137.
  • Rist, D.I., & Lorbeer, J.W. (1991). Relationships of weed reservoirs to cucumber mosaic virus (CMV) and broad bean wilt virus (BBWV) in commercial lettuce fields in New York. Phytopathology, 81(4):367-371.
  • Sayama, H., Kominato, M., Atarashi, H., Takayanagi, N., Yamada, M., Hikage, T., & Yoshike, T. (2006). Control of cucumber mosaic virus (CMV) in gentian by satellite RNA containing attenuated CMV strains. Acta Horticulturea, 722:147-154.
  • Takami, K., Okubo, H., Yamasaki, S., Takeshita, M., & Takanami, Y. (2006). A cucumber mosaicvirus isolated from Momordica charantia L. Journal of General Plant Pathology, 72(6):391-392.
  • Tessitori, M., Reina, A., Catara, V., & Polizzi, G. (2002). Polygala myrtifolia as a new natural host of Cucumber mosaic virus. Plant Disease, 86(12):1403.
  • Verma, N., Mahinghara, B.K., Ram, R., & Zaidi, A.A. (2006). Coat protein sequence shows that Cucumber mosaic virus isolate from geraniums (Pelargonium spp.) belongs to subgroup II. Journal of Biosciences, 31(1):47-54.
There are 28 citations in total.

Details

Journal Section Research Articles
Authors

Gökmen Koç

Hakan Fidan

Publication Date June 1, 2017
Published in Issue Year 2017 Volume: 34 Issue: 1

Cite

APA Koç, G., & Fidan, H. (2017). Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia. Derim, 34(1), 29-36. https://doi.org/10.16882/derim.2017.305281
AMA Koç G, Fidan H. Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia. DERİM. June 2017;34(1):29-36. doi:10.16882/derim.2017.305281
Chicago Koç, Gökmen, and Hakan Fidan. “Türkiye’de Cucumber Mosaic Virus (CMV) için Herdem yeşil Bir konukçu: Polygala Myrtifolia”. Derim 34, no. 1 (June 2017): 29-36. https://doi.org/10.16882/derim.2017.305281.
EndNote Koç G, Fidan H (June 1, 2017) Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia. Derim 34 1 29–36.
IEEE G. Koç and H. Fidan, “Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia”, DERİM, vol. 34, no. 1, pp. 29–36, 2017, doi: 10.16882/derim.2017.305281.
ISNAD Koç, Gökmen - Fidan, Hakan. “Türkiye’de Cucumber Mosaic Virus (CMV) için Herdem yeşil Bir konukçu: Polygala Myrtifolia”. Derim 34/1 (June 2017), 29-36. https://doi.org/10.16882/derim.2017.305281.
JAMA Koç G, Fidan H. Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia. DERİM. 2017;34:29–36.
MLA Koç, Gökmen and Hakan Fidan. “Türkiye’de Cucumber Mosaic Virus (CMV) için Herdem yeşil Bir konukçu: Polygala Myrtifolia”. Derim, vol. 34, no. 1, 2017, pp. 29-36, doi:10.16882/derim.2017.305281.
Vancouver Koç G, Fidan H. Türkiye’de Cucumber Mosaic Virus (CMV) için herdem yeşil bir konukçu: Polygala myrtifolia. DERİM. 2017;34(1):29-36.

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