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Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler

Year 2017, Volume: 10 Issue: 1, 1 - 6, 19.02.2017

Abstract

Nil tilapiası, Oreochromis niloticus (L.), Cladophora glomerata (L) Kutz (Chlorophyta)’nın bulunup bulunmadığı ortamlarda
kadmiyumun (0,1 mg/l ve 1 mg/l) sublethal konsantrasyonlarına maruz bırakıldı. 15 ve 30 günlük periyotlar sonunda Oreochromis
niloticus karaciğerleri alınarak histolojik preparatları hazırlandı. Kadmiyumun farklı konsantrasyonlarında karaciğerlerde meydana
gelen histopatolojik değişiklikler ışık mikroskobunda incelendi. Kadmiyum uygulaması sonucunda; karaciğer dokusunda, sinüzoidal
bölgelerde dilatasyon, kan damarlarında ve sinüzoidlerde konjesyon, hepatositlerde hipertrofi, hiyalin damlacıkları akümülasyonu,
parankim dejenerasyonu ve lipid vakuolasyonu gibi değişiklikler gözlendi. Ayrıca subkapsüler ve dağınık fokal nekroz görüldü.
Karaciğerlerde lezyonların şiddeti artan kadmiyum konsantrasyonuna ve zamana bağlı olarak artış gösterdi. 

References

  • 1. Aksoy A, Demirezen D, Duman F. (2005). Bioaccumulation, Detection and Analyses of Heavy Metal Pollution in Sultan Marsh and Its Environment. Water Air & Soil Pollution. 164: 241-255.
  • 2. Garcia-Santos S, Fontainhas-Fernandes A, Wilson JM. (2006). Cadmium Tolerance in the Nile tilapia (Oreochromis niloticus) Following Acute Exposure: Assessment of some Ionoregulatory Parameters. Environmental Toxicology. 21: 33-46.
  • 3. Almeida JA, Novelli KB, Silva MDP, Junıor RA. (2001). Environmental Cadmium Exposure and Metabolic Responses of Nile Tilapia, Oreochromis niloticus, Environmental Pollution. 114: 169-175.
  • 4. Kargin F, Cogun HY. (1999). Metal Interactions During Accumulation and Elimination of Zinc and Cadmium in Tissues of Zthe Freshwater Fish Tilapia nilotica. Bulletin of Environmental Contamination and Toxicology. 63, 546-552.
  • 5. Mehta S, Gaur JP. (2005). Use of Algae for Removing Heavy Metal Ions from Wastewater: Progress and Prospects. Critical Reviews in Biotechnology. 25: 113-152.
  • 6. McHardy BM, George JJ. (1990). Bioaccumulation and Toxicity of Zinc in The Green Alga, Cladophora glomerata. Environmental Pollution. 66: 55-66.
  • 7. Yalçın E, Çavuşoğlu K, Maraş M, Bıyıkoğlu M. (2008). Biosorption of Lead (II) and Copper (II) Metal Ions on Cladophora glomerata (L.) Kutz. (Chlorophyta) Algae: Effect of Algal Surface Modification. Acta Chimica Slovenica 55 (1): 228-232.
  • 8. Karadede Akın H, Ünlü E. (2013). Cadmium Accumulation by Green Algae Cladophora glomerata (L.) Kutz. (Chlorophyta) in Presence of Nile tilapia Oreochromis niloticus (L.), Toxicological and Environmental Chemistry. 95(9): 1565-1571.
  • 9. Heath AC. (1995). Water Pollution and Fish Physiology. 2nd Edn., Lewis Publishers, Boca Raton. 125-140.
  • 10. Gernhofer M, Pawet M, Schramm M, Müller E, Triebskorn R. (2001). Ultrastructural Biomarkers Tools to Characterize the Health Status of Fish in Contaminated Streams. Journal of Aquatic Ecosystem Stress and Recovery. 8: 241-260.
  • 11. Piyanut P, Maleeya K, Prayad P,Sombat S. (2008). Histopathological Alterations of Nile tilapia, Oreochromis niloticus in Acute and Subchronic Alachlor Exposure. Journal of Environmental Biology. 29 (3): 325-331.
  • 12. Al-Nasser LA. (2000). Cadmium Hepatotoxicity and Alterations of the Mitocondrial Function. Journal of Toxicology. Clinical Toxicology. 38: 407-413.
  • 13. Kaoud HA, Zaki MM, El-Dahshan AR, Saeid S, El Zorba HY. (2011). Amelioration the Toxic Effects of Cadmium-Exposure in Nile Tilapia (Oreochromis niloticus) by using Lemna gibba L. Life Science Journal. 8: 185-195.
  • 14. Selvanathan J, Vincent S, Nirmala A. (2012). Histopathology Changes in Fresh Water Fish Clarias batrachus (Linn.) Exposed to Mercury and Cadmium. International Journal of Pharmacy Teaching and Practices. 3 (4): 422-428.
  • 15. Omer S.A, Elobeid MA, Fouad D et al. (2012). Cadmium Bioaccumulation and Toxicity in Tilapia Fish (Oreochromis niloticus). Journal of Animal and Veterinary Advances 11 (10): 1601-1606.
  • 16. Stentiford G.D, Longshaw M, Lyons B.P. et al. 2003. Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants. Marine Environmental Research. 55: 137-159.
  • 17. Velkova-Jordanoska L, Kostoski G. (2005). Histopathological Analysis of Liver in Fish (Barbus meridionalis petenyi) Heckel in Reservoir Trebenita. Nature. Croatica. 14.(2): 147–153.
  • 18. Jiraungkoorskul W, Sahaphong S, Kangwanrangsn N, Huk Kim M. (2006). Histopathological Study: The Effect of Ascorbic Acid on Cadmium Exposure in Fish (Puntius altus). Journal of Fisheries and Aquatic Science. 1 (2): 191-199.
  • 19. Rashed MN. (2001). Monitoring of Environmental Heavy Metals in Fish from Nasser Lake. Environment International. 27: 27-33.
  • 20. Marafante, E. (1976). Binding of Mercury and Zinc to Cadmium-Binding Protein in Liver and Kidney of Gold Fish (Carassius auratus L.). Experientia. 32: 149-150.
  • 21. Bagchi D, Bagchi M, Hassoun EA and Stohs SJ. (1996). Cadmium Induced Excretion of Urinary Lipid Metabolites, DNA Damage, Glutathione Depletion and Hepatic Lipid Peroxidation in Sprague-Dawley rats. Biological Trace Element Research. 52: 143-154.
  • 22. Kabir SMH, Begum R. (1978). Toxicity of Three Organophosphorus Insecticides to Singhi Fish Heteropneustes fossilis (Bloch). Dhaka University Studies. B26: 115-122.
  • 23. Chmielewská E, Medved J. (2001). Bioaccumulation of heavy metals by green algae cladophora glomerata in a refinery sewage Lagoon. Croatica Chemical Acta. 74 (1), 135-145
  • 24. Andrade SALD, Jorge RA, Silveira APDD. (2005). Cadmium Effect on the Association of Jackbean (Canavalia ensiformis) and Arbuscular mycorrhizal fungi. Scientia Agricola. 62: 389-394.

Histological Changes in Liver Tissues of Nile Tilapia Oreochromis niloticus Exposed to Cadmium in the Presence of Green Algae Cladophora glomerata

Year 2017, Volume: 10 Issue: 1, 1 - 6, 19.02.2017

Abstract

Nile tilapia, Oreochromis niloticus (L.), was exposed to sublethal concentrations (0.1 mg/l and 1 mg/l) of cadmium (cd) in the presence or absence of Cladophora glomerata (L) Kutz (Chlorophyta). At the end of 15 and 30 days periods, Oreochromis niloticus samples were dissected and their livers were collected. Then, the livers were histologically investigated. Histopatological changes occurred in the livers at different concentrations of Cd were studied by light microscope. After Cd application, changes such as dilatation in the sinusoidal region of liver tissue, congestion in blood vessels and sinusoids, hypertrophy in hepatocytes, accumulation of hyaline droplets, degeneration of parenchyma and lipid vacuoiation were observed. Furthermore, subcapsular and dispersed focal necrosis were also encountered. Lesion intensity in the livers increased depending on increased Cd concentration and time. 

References

  • 1. Aksoy A, Demirezen D, Duman F. (2005). Bioaccumulation, Detection and Analyses of Heavy Metal Pollution in Sultan Marsh and Its Environment. Water Air & Soil Pollution. 164: 241-255.
  • 2. Garcia-Santos S, Fontainhas-Fernandes A, Wilson JM. (2006). Cadmium Tolerance in the Nile tilapia (Oreochromis niloticus) Following Acute Exposure: Assessment of some Ionoregulatory Parameters. Environmental Toxicology. 21: 33-46.
  • 3. Almeida JA, Novelli KB, Silva MDP, Junıor RA. (2001). Environmental Cadmium Exposure and Metabolic Responses of Nile Tilapia, Oreochromis niloticus, Environmental Pollution. 114: 169-175.
  • 4. Kargin F, Cogun HY. (1999). Metal Interactions During Accumulation and Elimination of Zinc and Cadmium in Tissues of Zthe Freshwater Fish Tilapia nilotica. Bulletin of Environmental Contamination and Toxicology. 63, 546-552.
  • 5. Mehta S, Gaur JP. (2005). Use of Algae for Removing Heavy Metal Ions from Wastewater: Progress and Prospects. Critical Reviews in Biotechnology. 25: 113-152.
  • 6. McHardy BM, George JJ. (1990). Bioaccumulation and Toxicity of Zinc in The Green Alga, Cladophora glomerata. Environmental Pollution. 66: 55-66.
  • 7. Yalçın E, Çavuşoğlu K, Maraş M, Bıyıkoğlu M. (2008). Biosorption of Lead (II) and Copper (II) Metal Ions on Cladophora glomerata (L.) Kutz. (Chlorophyta) Algae: Effect of Algal Surface Modification. Acta Chimica Slovenica 55 (1): 228-232.
  • 8. Karadede Akın H, Ünlü E. (2013). Cadmium Accumulation by Green Algae Cladophora glomerata (L.) Kutz. (Chlorophyta) in Presence of Nile tilapia Oreochromis niloticus (L.), Toxicological and Environmental Chemistry. 95(9): 1565-1571.
  • 9. Heath AC. (1995). Water Pollution and Fish Physiology. 2nd Edn., Lewis Publishers, Boca Raton. 125-140.
  • 10. Gernhofer M, Pawet M, Schramm M, Müller E, Triebskorn R. (2001). Ultrastructural Biomarkers Tools to Characterize the Health Status of Fish in Contaminated Streams. Journal of Aquatic Ecosystem Stress and Recovery. 8: 241-260.
  • 11. Piyanut P, Maleeya K, Prayad P,Sombat S. (2008). Histopathological Alterations of Nile tilapia, Oreochromis niloticus in Acute and Subchronic Alachlor Exposure. Journal of Environmental Biology. 29 (3): 325-331.
  • 12. Al-Nasser LA. (2000). Cadmium Hepatotoxicity and Alterations of the Mitocondrial Function. Journal of Toxicology. Clinical Toxicology. 38: 407-413.
  • 13. Kaoud HA, Zaki MM, El-Dahshan AR, Saeid S, El Zorba HY. (2011). Amelioration the Toxic Effects of Cadmium-Exposure in Nile Tilapia (Oreochromis niloticus) by using Lemna gibba L. Life Science Journal. 8: 185-195.
  • 14. Selvanathan J, Vincent S, Nirmala A. (2012). Histopathology Changes in Fresh Water Fish Clarias batrachus (Linn.) Exposed to Mercury and Cadmium. International Journal of Pharmacy Teaching and Practices. 3 (4): 422-428.
  • 15. Omer S.A, Elobeid MA, Fouad D et al. (2012). Cadmium Bioaccumulation and Toxicity in Tilapia Fish (Oreochromis niloticus). Journal of Animal and Veterinary Advances 11 (10): 1601-1606.
  • 16. Stentiford G.D, Longshaw M, Lyons B.P. et al. 2003. Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants. Marine Environmental Research. 55: 137-159.
  • 17. Velkova-Jordanoska L, Kostoski G. (2005). Histopathological Analysis of Liver in Fish (Barbus meridionalis petenyi) Heckel in Reservoir Trebenita. Nature. Croatica. 14.(2): 147–153.
  • 18. Jiraungkoorskul W, Sahaphong S, Kangwanrangsn N, Huk Kim M. (2006). Histopathological Study: The Effect of Ascorbic Acid on Cadmium Exposure in Fish (Puntius altus). Journal of Fisheries and Aquatic Science. 1 (2): 191-199.
  • 19. Rashed MN. (2001). Monitoring of Environmental Heavy Metals in Fish from Nasser Lake. Environment International. 27: 27-33.
  • 20. Marafante, E. (1976). Binding of Mercury and Zinc to Cadmium-Binding Protein in Liver and Kidney of Gold Fish (Carassius auratus L.). Experientia. 32: 149-150.
  • 21. Bagchi D, Bagchi M, Hassoun EA and Stohs SJ. (1996). Cadmium Induced Excretion of Urinary Lipid Metabolites, DNA Damage, Glutathione Depletion and Hepatic Lipid Peroxidation in Sprague-Dawley rats. Biological Trace Element Research. 52: 143-154.
  • 22. Kabir SMH, Begum R. (1978). Toxicity of Three Organophosphorus Insecticides to Singhi Fish Heteropneustes fossilis (Bloch). Dhaka University Studies. B26: 115-122.
  • 23. Chmielewská E, Medved J. (2001). Bioaccumulation of heavy metals by green algae cladophora glomerata in a refinery sewage Lagoon. Croatica Chemical Acta. 74 (1), 135-145
  • 24. Andrade SALD, Jorge RA, Silveira APDD. (2005). Cadmium Effect on the Association of Jackbean (Canavalia ensiformis) and Arbuscular mycorrhizal fungi. Scientia Agricola. 62: 389-394.
There are 24 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research
Authors

Birgül Otludil This is me

Hülya Karadede Akın

Publication Date February 19, 2017
Published in Issue Year 2017 Volume: 10 Issue: 1

Cite

APA Otludil, B., & Karadede Akın, H. (2017). Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 10(1), 1-6.
AMA Otludil B, Karadede Akın H. Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler. Dicle Üniv Vet Fak Derg. February 2017;10(1):1-6.
Chicago Otludil, Birgül, and Hülya Karadede Akın. “Yeşil Alg Cladophora Glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10, no. 1 (February 2017): 1-6.
EndNote Otludil B, Karadede Akın H (February 1, 2017) Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10 1 1–6.
IEEE B. Otludil and H. Karadede Akın, “Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler”, Dicle Üniv Vet Fak Derg, vol. 10, no. 1, pp. 1–6, 2017.
ISNAD Otludil, Birgül - Karadede Akın, Hülya. “Yeşil Alg Cladophora Glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10/1 (February 2017), 1-6.
JAMA Otludil B, Karadede Akın H. Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler. Dicle Üniv Vet Fak Derg. 2017;10:1–6.
MLA Otludil, Birgül and Hülya Karadede Akın. “Yeşil Alg Cladophora Glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 10, no. 1, 2017, pp. 1-6.
Vancouver Otludil B, Karadede Akın H. Yeşil Alg Cladophora glomerata Mevcudiyetinde, Kadmiuma Maruz Bırakılan Nil tilapiası, Oreochromis niloticus’un Karaciğer Dokularındaki Histolojik Değişiklikler. Dicle Üniv Vet Fak Derg. 2017;10(1):1-6.