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Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits

Year 2020, Volume: 13 Issue: 2, 92 - 98, 31.12.2020
https://doi.org/10.47027/duvetfd.735544

Abstract

Centarium erythraea, a species of flowering plant in the Gentianaceae family, is a plant commonly used in the wound treatment. This study aims to investigate the effect of Centarium erythraea in enhancing healing process in full-thickness skin wounds in diabetic rabbits. A total of 28 young (6 months and body weight of 2.5-3 kg) female New Zealand rabbits were used. Diabetes mellitus was created by injection of alloxan monohydrate. After 15 days following diabetes induction, the surgical operation was applied under general anesthesia and two full-thickness 2.6 x 2.6 cm wounds were created that were equidistant from the dorsal median line. A total of four groups were formed with 7 rabbits in each group. For wounds, on a daily basis, oily homogenized form of Centarium erythraea was administered in Group 1, while pomade form of the titrated extract of Centella asiatica in Group 2, pure olive oil in Group 3 and normal saline was applied in Group 4. Wound edges were measured on the 4, 8, 12, 16, 20 and 24th days, furthermore, histopathological and genetic examinations were performed on tissue samples taken on the same days. Examining the wound diameters, healing rates in the Group 1 and Group 2 were found statistically significant compared to Group 3 and Group 4 (p<0,05). In histopathological examinations, it was noted that connective tissue increase, collagen proliferation and epitelogenesis were significant in Groups 1 and 2 compared to Groups 3 and 4 (p<0.05). Inflammatory cell infiltrations, hyperemia and necrosis in Groups 3 and Group 4 were observed with a high intensity compared to Groups 1 and Group 2 (p<0.05). IL-6, IL-8 and CXCR1 gene expressions were observed to be low in all diabetic groups. Although the expression values were low in groups 1 and 2, it was found that there was a significant difference in the expression amounts of the target genes on the 4 and 8 days compared to other groups (p <0.05). As a result, when the healing rate in the full-thickness skin wounds in the diabetic rabbits is examined at the level of wound diameters, histopathology and gene expression, it occurred in order of Group 2, Group 1, Group 3 and Group 4. Oily homogenized form of Centarium erythraea was found to have a positive effect in full-thickness wound healing of diabetic rabbits.

References

  • 1. Hamza N, Berke B, Cheze C, et al. (2010). Prevention of Type 2 Diabetes İnduced by High Fat Diet in the C57BL/6J Mouse by Two Medicinal Plants Used in Traditional Treatment of Diabetes in the East of Algeria. J Ethnopharmacol. 128(2), 513-518.
  • 2. Perez-Nieves M, Ivanova JI, Hadjiyianni I, et al. (2017), Basal İnsulin İnitiation Use and Experience Among People with Type 2 Diabetes Mellitus with Different Patterns of Persistence: Results From a Multinational Survey. Curr Med Res Opin. 33(10): 1833-42.
  • 3. Yadav M, Sharma N, Garg A, et al. (2017), Herbal Drugs and Phytoconstituents Useful for the Management of Diabetes. Int. J. Green Pharm. 11(1): 21-29.
  • 4. Chouhan D, Janani G, Chakraborty B, Nandi SK, Mandal BB. (2018). Functionalized PVA–Silk Blended Nanofibrous Mats Promote Diabetic Wound Healing via Regulation of Extracellular Matrix And Tissue Remodelling. J Tissue Eng Regen Med. 12(3): 1559-1570.
  • 5. Yadollah-Damavandi S, Chavoshi-Nejad M, Jangholi E, et al. (2015), Topical Hypericum perforatum Improves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model. J Evid Based Complementary Altern Med. Article ID 245328: 1-4.
  • 6. McCarthy ME, Brown TA, Bukowska J, et al. (2018). Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering. Curr Stem Cell Rep. 4(2): 127-137.
  • 7. Pradhan L, Cai X, Wu S, et al. (2011). Gene Expression of Pro-İnflammatory Cytokines and Neuropeptides in Diabetic Wound Healing. J Surg Res. 167(2): 336-342.
  • 8. Luckett-Chastain LR, Gipson JR, Gillaspy AF, Gallucci RM. (2018). Transcriptional Profiling of Irritant Contact Dermatitis (ICD) in a Mouse Model Identifies Specific Patterns of Gene Expression and İmmune-Regulation. Toxicology. 410: 1-9.
  • 9. Law JX, Chowdhury SR, Aminuddin BS, Ruszymah BHI. (2017). Role of Plasma-Derived Fibrin on Keratinocyte and Fibroblast Wound Healing. Cell Tissue Bank. 18(4): 585-595.
  • 10. Shi HX, Lin C, Lin BB, et al. (2013). The Anti-Scar Effects of Basic Fibroblast Growth Factor on The Wound Repair in Vitro and in Vivo. PloS one, 8(4): e59966.
  • 11. Xu C, Rosler E, Jiang J, et al. (2005). Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned Medium. Stem cells, 23(3): 315-323.
  • 12. Vatankhah N, Jahangiri Y, Landry GJ, Moneta GL, Azarbal AF. (2017). Effect of Systemic Insulin Treatment on Diabetic Wound Healing. Wound Repair Regen, 25(2): 288-291.
  • 13. Arulselvana P, Abdul Ghofara HA, Karthivashana G, Abdul Halima MF, Abdul Ghafara MS, Fakurazia S. (2014). Antidiabetic Therapeutics From Natural Source: A Systematic Review. Biomedicine & Preventive Nutrition. (4): 607–617.
  • 14. Arumugam G, Manjula P, Paari N. (2013). A Review: Anti Diabetic Medicinal Plants Used For Diabetes Mellitus. J. Acute Dis. 2(3): 196-200.
  • 15. Grover JK, Yadav S, Vats V. (2002). Medicinal Plants of India with Anti-Diabetic Potential, J Ethnopharmacol. 81 (1): 81-100.
  • 16. Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2001). Antioxidant Activity of Centaurium erythraea Infusion Evidenced by Its Superoxide Radical Scavenging and Xanthine Oxidase Inhibitory Activity. J. Agric. Food Chem. 49: 3476−79.
  • 17. Tahraoui A, Israili ZH, Lyoussi B. (2010). Acute and Sub-Chronic Toxicity of a Lyophilised Aqueous Extract of Centaurium erythraea in Rodents. J Ethnopharmacol. 132(1):48-55.
  • 18. Springefeld K. Centaurium erythraea-The medicinal plant of the year 2004. Pharm. Ztg. 149: 30.
  • 19. Šiler B, Mišić D. (2016). Biologically Active Compounds from the Genus Centaurium sl (Gentianaceae): Current Knowledge and Future Prospects in Medicine. In: Studies in Natural Products Chemistry. Vol. 49, pp. 363-397. Elsevier, USA.
  • 20. Tuluce Y, Ozkol H, Koyuncu I, Ine H. (2011). Gastroprotective Effect of Small Centaury (Centaurium Erythraea L) on Aspirin-İnduced Gastric Damage in Rats. Toxıcol Ind Health. 27(8), 760-768.
  • 21. Berkan T, Üstünes L, Lermioglu F, Özer A. (1991). Antiinflammatory, Analgesic, and Antipyretic Effects of an Aqueous Extract of Erythraea centaurium. Planta Med. 57: 34-37.
  • 22. Moore N, Hamza N, Berke B, Umar A. (2017). News from Tartary: an Ethnopharmacological Approach to Drug and Therapeutic Discovery. Br. J. Clin. Pharmacol. 83(1): 33-37.
  • 23. Schouppe D, Brys R, Vallejo-Marin M, Jacquemyn H. (2017). Geographic Variation in Floral Traits and the Capacity of Autonomous Selfing Across Allopatric and Sympatric Populations of Two Closely Related Centaurium Species. Sci. Rep. 7: 46410.
  • 24. Kumarasamy Y, Nahar L, Cox PJ, Jaspars M, Sarker SD. (2003). Bioactivity of Secoiridoid Glycosides from Centaurium erythraea. Phytomedicine. 10(4): 344-347.
  • 25. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2003). Hydroxyl Radical and Hypochlorous Acid Scavenging Activity of Small Centaury (Centaurium erythraea) Infusion. A Comparative Study with Green Tea (Camellia sinensis). Phytomedicine. 10: 517–522.
  • 26. Kumarasamy Y, Nahar L, Sarker SD. (2003). Bioactivity of Gentiopicroside from the Aerial Parts of Centaurium erythraea. Fitoterapia. 74: 151–154.
  • 27. Stefkov G, Miova B, Dinevska-Kjovkarovska S, et al. (2014). Chemical Characterization of Centaurium Erythrea L. and its Effects on Carbohydrate and Lipid Metabolism in Experimental Diabetes. J Ethnopharmacol. 152: 71-77.
  • 28. Banjanac T, Dragićević M, Šiler B, et al. (2017). Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search For High-Resolution Taxonomic Classifiers. Phytochemistry. 140: 27-44.
  • 29. Breen A, Mc Redmond G, Dockery P, O’Brien T, Pandit A. (2008). Assessment of Wound Healing in the Alloxan-İnduced Diabetic Rabbit Ear Model. J Invest Surg 21(5): 261-9.
  • 30. Han MC, Durmuş AS, Sağlıyan A, et al. (2017). Effects of Nigella sativa and Hypericum perforatum on wound healing. Türk J Vet Anım Sci, 41: 99-105.
  • 31. Hassani FV, Naseri V, Razavi BM, Mehri S, Abnous K, Hosseinzadeh H. (2014). Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus. DARU Journal of Pharmaceutical Sciences. 22(1): 16.
  • 32. Karaca ZM, Ozen H, Akgoz M. Cigremis Y. (2018). Effect of caffeic acid phenethyl ester (CAPE) on vascular endothelial growth factor a (VEGF-A) gene expression in gentamicin-induced acute renal nephrotoxicity. Medicine. 7(4): 805-9.
  • 33. Słotwiński R, Sarnecka A, Dąbrowska A, et al. (2015). Innate İmmunity Gene Expression Changes in Critically İll Patients with Sepsis and Disease-Related Malnutrition. Cent Eur J Immunol. 40(3): 311.
  • 34. Hamza N, Berke B, Cheze C, et al. (2011). Treatment of High Fat Diet İnduced Type 2 Diabetes in C57BL/6J Mice by Two Medicinal Plants Used in Traditional Treatment of Diabetes in The East of Algeria. J Ethnopharmacol. 133(2): 931-933.
  • 35. Gopinath D, Ahmeda MR, Gomathia K, Chitraa K, Sehgalb PK, Jayakumara R. (2004). Dermal Wound Healing Processes with Curcumin İncorporated Collagen Films. Biomaterials. 25: 1911–1917.
  • 36. Shukla A, Rasik AM, Dhawan BN. (1999). Asiaticoside-induced Elevation of Antioxidant Levels in Healing Wounds. Phytother. Res. 13: 50–54.
  • 37. Maquart FX, Bellon G, Wegrowski Y, Barel JP. (1990). Stimulation of Collagen Synthesis in Fibroblast Culture by a Triterpene Extracted From Centella asiatica. Conn. Tissue Res. 24: 107-120.
  • 38. Nabzdyk LP, Kuchibhotla S, Guthrie P, et al. (2013). Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. J. Vasc. Surg. 58(3), 766-775.
  • 39. Lin ZQ, Kondo T, Ishida Y, Takayasu T, Mukaida N. (2003). Essential Involvement of IL‐6 in the Skin Wound‐Healing Process as Evidenced by Delayed Wound Healing in IL‐6‐Deficient Mice. J. Leukoc. Biol. 73(6): 713-721.
  • 40. Lan CCE, Wu, CS, Huang SM, Wu, IH, Chen GS. (2013). High-Glucose Environment Enhanced Oxidative Stress and Increased Interleukin-8 Secretion from Keratinocytes: New Insights into Impaired Diabetic Wound Healing. Diabetes. 62(7): 2530-2538.
  • 41. El Euch SK, Cieśla Ł, Bouzouita N. (2014). Free Radical Scavenging Fingerprints of Selected Aromatic and Medicinal Tunisian Plants Assessed by Means of TLC-DPPH• Test and Image Processing. J. AOAC Int. 97(5): 1291-1298.
  • 42. Sefi M, Fetoui H, Lachkar N, et al. (2011). Centaurium erythrea (Gentianaceae) Leaf Extract Alleviates Streptozotocin-İnduced Oxidative Stress and Β-Cell Damage in Rat Pancreas. J. Ethnopharmacol. 135(2): 243-250.
  • 43. Shetty S, Udupa S, Udupa L. (2008). Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum Sanctum Linn in Rats. Evid. Based Complementary Altern. Med. 5(1): 95-101.
  • 44. Cos P, Ying L, Calomme M, et al. (1998). Structure−activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine Oxidase and Superoxide Scavengers. J. Nat. Prod. 61(1): 71-76.

Diyabetik Tavşanların Tam Katlı Deri Yaralarında Topikal Centarium Erythraea’nın Etkisinin Araştırılması

Year 2020, Volume: 13 Issue: 2, 92 - 98, 31.12.2020
https://doi.org/10.47027/duvetfd.735544

Abstract

Gentianaceae ailesine ait olan Centarium erythraea yara tedavisinde halk arasında sık kullanılan bir bitkidir. Bu çalışmanın amacı Centarium erythraea’nın diyabet oluşturulmuş tavşanların tam katlı deri yaralarında yara iyileşmesi üzerine etkisini araştırmaktır. Çalışmada 6 aylık yaşta, dişi, ortalama ağırlığı 2.5-3 kg olan 28 adet Yeni Zelanda tavşanına alloksan monohidrat enjeksiyonlarıyla diyabet oluşturuldu. Diyabet indüksiyonundan 15 gün sonra genel anestezi altında cerrahi işlem uygulanarak dorsal median hattan eşit uzaklıkta iki adet 2,6 x 2.6 cm çapında tam katlı deri yaraları oluşturuldu. Tavşanlar her grupta 7 tavşan olacak şekilde 4 gruba ayrıldı. Yaralara günlük olarak Grup 1 Centarium erythraea’nın yağlı homojenize formu, Grup 2 Centella asiatica’nın titre edilmiş ekstraktının pomat formu, Grup 3 saf zeytinyağı ve Grup 4 serum fizyolojik uygulandı. Yara sınırları 4, 8, 12, 16, 20 ve 24. günlerde ölçülürken aynı günlerde alınan doku örneklerinde histopatolojik ve genetik incelemeler yapıldı. Çalışma sonunda yara çapları incelendiğinde Grup 1 ve Grup 2’de yara iyileşme hızı Grup 3 ve Grup 4’e oranla istatistiksel olarak önemli bulundu (p<0,05). Histopatolojik incelemelerde, bağ doku artışı kollajen proliferasyonu ve epitelogenezisin Grup 1 ve 2’de Grup 3 ve 4’e oranla belirgin olduğu gözlendi (p<0,05). Grup 3 ve 4’de yangısal hücre infiltrasyonları, hiperemi ve nekrozun Grup 1 ve 2’ye göre şiddetli yoğunlukta gözlendi (p<0.05). IL-6, IL-8 ve CXCR1 gen ekspresyonlarının tüm diabetli gruplarda düşük olduğu gözlendi. Grup 1 ve 2’de ekspresyon değerleri düşük olmasına rağmen 4 ve 8. günlerde diğer gruplara göre hedef genlerin ekspresyon miktarlarında anlamlı bir farklılık olduğu tespit edildi (p<0.05). Sonuç olarak diyabetli tavşanların tam katlı deri yaralarında iyileşme hızı yara çapları, histopatoloji ve gen ekspresyon düzeyinde incelendiğinde Grup 2, Grup 1, Grup 3 ve Grup 4 sırasıyla gerçekleşti. Centarium erythraea’nın yağlı homojenize formunun diyabetik tavşanların tam katlı deri yaralarında yara iyileşmesine olumlu etkisinin olduğu görüldü.

References

  • 1. Hamza N, Berke B, Cheze C, et al. (2010). Prevention of Type 2 Diabetes İnduced by High Fat Diet in the C57BL/6J Mouse by Two Medicinal Plants Used in Traditional Treatment of Diabetes in the East of Algeria. J Ethnopharmacol. 128(2), 513-518.
  • 2. Perez-Nieves M, Ivanova JI, Hadjiyianni I, et al. (2017), Basal İnsulin İnitiation Use and Experience Among People with Type 2 Diabetes Mellitus with Different Patterns of Persistence: Results From a Multinational Survey. Curr Med Res Opin. 33(10): 1833-42.
  • 3. Yadav M, Sharma N, Garg A, et al. (2017), Herbal Drugs and Phytoconstituents Useful for the Management of Diabetes. Int. J. Green Pharm. 11(1): 21-29.
  • 4. Chouhan D, Janani G, Chakraborty B, Nandi SK, Mandal BB. (2018). Functionalized PVA–Silk Blended Nanofibrous Mats Promote Diabetic Wound Healing via Regulation of Extracellular Matrix And Tissue Remodelling. J Tissue Eng Regen Med. 12(3): 1559-1570.
  • 5. Yadollah-Damavandi S, Chavoshi-Nejad M, Jangholi E, et al. (2015), Topical Hypericum perforatum Improves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model. J Evid Based Complementary Altern Med. Article ID 245328: 1-4.
  • 6. McCarthy ME, Brown TA, Bukowska J, et al. (2018). Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering. Curr Stem Cell Rep. 4(2): 127-137.
  • 7. Pradhan L, Cai X, Wu S, et al. (2011). Gene Expression of Pro-İnflammatory Cytokines and Neuropeptides in Diabetic Wound Healing. J Surg Res. 167(2): 336-342.
  • 8. Luckett-Chastain LR, Gipson JR, Gillaspy AF, Gallucci RM. (2018). Transcriptional Profiling of Irritant Contact Dermatitis (ICD) in a Mouse Model Identifies Specific Patterns of Gene Expression and İmmune-Regulation. Toxicology. 410: 1-9.
  • 9. Law JX, Chowdhury SR, Aminuddin BS, Ruszymah BHI. (2017). Role of Plasma-Derived Fibrin on Keratinocyte and Fibroblast Wound Healing. Cell Tissue Bank. 18(4): 585-595.
  • 10. Shi HX, Lin C, Lin BB, et al. (2013). The Anti-Scar Effects of Basic Fibroblast Growth Factor on The Wound Repair in Vitro and in Vivo. PloS one, 8(4): e59966.
  • 11. Xu C, Rosler E, Jiang J, et al. (2005). Basic Fibroblast Growth Factor Supports Undifferentiated Human Embryonic Stem Cell Growth Without Conditioned Medium. Stem cells, 23(3): 315-323.
  • 12. Vatankhah N, Jahangiri Y, Landry GJ, Moneta GL, Azarbal AF. (2017). Effect of Systemic Insulin Treatment on Diabetic Wound Healing. Wound Repair Regen, 25(2): 288-291.
  • 13. Arulselvana P, Abdul Ghofara HA, Karthivashana G, Abdul Halima MF, Abdul Ghafara MS, Fakurazia S. (2014). Antidiabetic Therapeutics From Natural Source: A Systematic Review. Biomedicine & Preventive Nutrition. (4): 607–617.
  • 14. Arumugam G, Manjula P, Paari N. (2013). A Review: Anti Diabetic Medicinal Plants Used For Diabetes Mellitus. J. Acute Dis. 2(3): 196-200.
  • 15. Grover JK, Yadav S, Vats V. (2002). Medicinal Plants of India with Anti-Diabetic Potential, J Ethnopharmacol. 81 (1): 81-100.
  • 16. Valentao P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2001). Antioxidant Activity of Centaurium erythraea Infusion Evidenced by Its Superoxide Radical Scavenging and Xanthine Oxidase Inhibitory Activity. J. Agric. Food Chem. 49: 3476−79.
  • 17. Tahraoui A, Israili ZH, Lyoussi B. (2010). Acute and Sub-Chronic Toxicity of a Lyophilised Aqueous Extract of Centaurium erythraea in Rodents. J Ethnopharmacol. 132(1):48-55.
  • 18. Springefeld K. Centaurium erythraea-The medicinal plant of the year 2004. Pharm. Ztg. 149: 30.
  • 19. Šiler B, Mišić D. (2016). Biologically Active Compounds from the Genus Centaurium sl (Gentianaceae): Current Knowledge and Future Prospects in Medicine. In: Studies in Natural Products Chemistry. Vol. 49, pp. 363-397. Elsevier, USA.
  • 20. Tuluce Y, Ozkol H, Koyuncu I, Ine H. (2011). Gastroprotective Effect of Small Centaury (Centaurium Erythraea L) on Aspirin-İnduced Gastric Damage in Rats. Toxıcol Ind Health. 27(8), 760-768.
  • 21. Berkan T, Üstünes L, Lermioglu F, Özer A. (1991). Antiinflammatory, Analgesic, and Antipyretic Effects of an Aqueous Extract of Erythraea centaurium. Planta Med. 57: 34-37.
  • 22. Moore N, Hamza N, Berke B, Umar A. (2017). News from Tartary: an Ethnopharmacological Approach to Drug and Therapeutic Discovery. Br. J. Clin. Pharmacol. 83(1): 33-37.
  • 23. Schouppe D, Brys R, Vallejo-Marin M, Jacquemyn H. (2017). Geographic Variation in Floral Traits and the Capacity of Autonomous Selfing Across Allopatric and Sympatric Populations of Two Closely Related Centaurium Species. Sci. Rep. 7: 46410.
  • 24. Kumarasamy Y, Nahar L, Cox PJ, Jaspars M, Sarker SD. (2003). Bioactivity of Secoiridoid Glycosides from Centaurium erythraea. Phytomedicine. 10(4): 344-347.
  • 25. Valentão P, Fernandes E, Carvalho F, Andrade PB, Seabra RM, Bastos ML. (2003). Hydroxyl Radical and Hypochlorous Acid Scavenging Activity of Small Centaury (Centaurium erythraea) Infusion. A Comparative Study with Green Tea (Camellia sinensis). Phytomedicine. 10: 517–522.
  • 26. Kumarasamy Y, Nahar L, Sarker SD. (2003). Bioactivity of Gentiopicroside from the Aerial Parts of Centaurium erythraea. Fitoterapia. 74: 151–154.
  • 27. Stefkov G, Miova B, Dinevska-Kjovkarovska S, et al. (2014). Chemical Characterization of Centaurium Erythrea L. and its Effects on Carbohydrate and Lipid Metabolism in Experimental Diabetes. J Ethnopharmacol. 152: 71-77.
  • 28. Banjanac T, Dragićević M, Šiler B, et al. (2017). Chemodiversity of Two Closely Related Tetraploid Centaurium Species and Their Hexaploid Hybrid: Metabolomic Search For High-Resolution Taxonomic Classifiers. Phytochemistry. 140: 27-44.
  • 29. Breen A, Mc Redmond G, Dockery P, O’Brien T, Pandit A. (2008). Assessment of Wound Healing in the Alloxan-İnduced Diabetic Rabbit Ear Model. J Invest Surg 21(5): 261-9.
  • 30. Han MC, Durmuş AS, Sağlıyan A, et al. (2017). Effects of Nigella sativa and Hypericum perforatum on wound healing. Türk J Vet Anım Sci, 41: 99-105.
  • 31. Hassani FV, Naseri V, Razavi BM, Mehri S, Abnous K, Hosseinzadeh H. (2014). Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus. DARU Journal of Pharmaceutical Sciences. 22(1): 16.
  • 32. Karaca ZM, Ozen H, Akgoz M. Cigremis Y. (2018). Effect of caffeic acid phenethyl ester (CAPE) on vascular endothelial growth factor a (VEGF-A) gene expression in gentamicin-induced acute renal nephrotoxicity. Medicine. 7(4): 805-9.
  • 33. Słotwiński R, Sarnecka A, Dąbrowska A, et al. (2015). Innate İmmunity Gene Expression Changes in Critically İll Patients with Sepsis and Disease-Related Malnutrition. Cent Eur J Immunol. 40(3): 311.
  • 34. Hamza N, Berke B, Cheze C, et al. (2011). Treatment of High Fat Diet İnduced Type 2 Diabetes in C57BL/6J Mice by Two Medicinal Plants Used in Traditional Treatment of Diabetes in The East of Algeria. J Ethnopharmacol. 133(2): 931-933.
  • 35. Gopinath D, Ahmeda MR, Gomathia K, Chitraa K, Sehgalb PK, Jayakumara R. (2004). Dermal Wound Healing Processes with Curcumin İncorporated Collagen Films. Biomaterials. 25: 1911–1917.
  • 36. Shukla A, Rasik AM, Dhawan BN. (1999). Asiaticoside-induced Elevation of Antioxidant Levels in Healing Wounds. Phytother. Res. 13: 50–54.
  • 37. Maquart FX, Bellon G, Wegrowski Y, Barel JP. (1990). Stimulation of Collagen Synthesis in Fibroblast Culture by a Triterpene Extracted From Centella asiatica. Conn. Tissue Res. 24: 107-120.
  • 38. Nabzdyk LP, Kuchibhotla S, Guthrie P, et al. (2013). Expression of neuropeptides and cytokines in a rabbit model of diabetic neuroischemic wound healing. J. Vasc. Surg. 58(3), 766-775.
  • 39. Lin ZQ, Kondo T, Ishida Y, Takayasu T, Mukaida N. (2003). Essential Involvement of IL‐6 in the Skin Wound‐Healing Process as Evidenced by Delayed Wound Healing in IL‐6‐Deficient Mice. J. Leukoc. Biol. 73(6): 713-721.
  • 40. Lan CCE, Wu, CS, Huang SM, Wu, IH, Chen GS. (2013). High-Glucose Environment Enhanced Oxidative Stress and Increased Interleukin-8 Secretion from Keratinocytes: New Insights into Impaired Diabetic Wound Healing. Diabetes. 62(7): 2530-2538.
  • 41. El Euch SK, Cieśla Ł, Bouzouita N. (2014). Free Radical Scavenging Fingerprints of Selected Aromatic and Medicinal Tunisian Plants Assessed by Means of TLC-DPPH• Test and Image Processing. J. AOAC Int. 97(5): 1291-1298.
  • 42. Sefi M, Fetoui H, Lachkar N, et al. (2011). Centaurium erythrea (Gentianaceae) Leaf Extract Alleviates Streptozotocin-İnduced Oxidative Stress and Β-Cell Damage in Rat Pancreas. J. Ethnopharmacol. 135(2): 243-250.
  • 43. Shetty S, Udupa S, Udupa L. (2008). Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract of Ocimum Sanctum Linn in Rats. Evid. Based Complementary Altern. Med. 5(1): 95-101.
  • 44. Cos P, Ying L, Calomme M, et al. (1998). Structure−activity Relationship and Classification of Flavonoids as Inhibitors of Xanthine Oxidase and Superoxide Scavengers. J. Nat. Prod. 61(1): 71-76.
There are 44 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research
Authors

Ünal Yavuz 0000-0002-4981-2355

Füsun Temamoğulları 0000-0001-7738-1145

Akin Yığın 0000-0001-9758-1697

Nihat Yumuşak 0000-0002-9299-2902

Publication Date December 31, 2020
Acceptance Date September 20, 2020
Published in Issue Year 2020 Volume: 13 Issue: 2

Cite

APA Yavuz, Ü., Temamoğulları, F., Yığın, A., Yumuşak, N. (2020). Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(2), 92-98. https://doi.org/10.47027/duvetfd.735544
AMA Yavuz Ü, Temamoğulları F, Yığın A, Yumuşak N. Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits. Dicle Üniv Vet Fak Derg. December 2020;13(2):92-98. doi:10.47027/duvetfd.735544
Chicago Yavuz, Ünal, Füsun Temamoğulları, Akin Yığın, and Nihat Yumuşak. “Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13, no. 2 (December 2020): 92-98. https://doi.org/10.47027/duvetfd.735544.
EndNote Yavuz Ü, Temamoğulları F, Yığın A, Yumuşak N (December 1, 2020) Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13 2 92–98.
IEEE Ü. Yavuz, F. Temamoğulları, A. Yığın, and N. Yumuşak, “Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits”, Dicle Üniv Vet Fak Derg, vol. 13, no. 2, pp. 92–98, 2020, doi: 10.47027/duvetfd.735544.
ISNAD Yavuz, Ünal et al. “Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 13/2 (December 2020), 92-98. https://doi.org/10.47027/duvetfd.735544.
JAMA Yavuz Ü, Temamoğulları F, Yığın A, Yumuşak N. Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits. Dicle Üniv Vet Fak Derg. 2020;13:92–98.
MLA Yavuz, Ünal et al. “Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 13, no. 2, 2020, pp. 92-98, doi:10.47027/duvetfd.735544.
Vancouver Yavuz Ü, Temamoğulları F, Yığın A, Yumuşak N. Investigation of the Effects of Topical Centarium Erythraea in Full-Thickness Skin Wounds in Diabetic Rabbits. Dicle Üniv Vet Fak Derg. 2020;13(2):92-8.