Adana İlinde Sığırlarda Anaplasma marginale ve Anaplasma centrale’nin Serolojik ve Moleküler Yöntemler ile Araştırılması

Year 2019, Volume: 30 Issue: 2, 109 - 114, 27.12.2019

Tülin Guven Gokmen Elçin Günaydın Osman Sezer Pınar Ayvazoğlu Demir Armağan Erdem Ütük

https://doi.org/10.35864/evmd.631791

Abstract

Anaplazmozis
tropikal ve subtropikal iklim bölgelerinde vektörler tarafından taşınan yaygın
bir hastalıktır. Özellikle büyükbaş hayvancılık işletmelerinde son yıllarda sık
tespit edilmeye başlanmıştır. Bu çalışmanın amacı, Adana ilindeki sığırlarda Anaplasma spp. prevalansını belirlemekti.
Bu amaçla Adana'nın farklı iklim bölgelerine sahip on beş ilçesinde sığırlardan
187 kan örneği alınarak kompetetif ELISA (cELISA) ve Nested-PCR yöntemleriyle
incelenmiştir. Sığırlarda seropozitiflik%38,5 (72/187) olarak belirlenmiştir. Ayrıca
moleküler prevalans, Nested-PCR yöntemleriyle Anaplasma centrale için%1,6 (3/187), Anaplasma marjinale için%3,2 (6/187) olarak tespit edilmiştir. Bu
çalışma ile Türkiye'nin Adana ilinde sığır anaplazmozisi ile ilgili
epidemiyolojik verileri ayrıntılı bir şekilde ele alınmış ve elde edilen
verilerin hastalığın önlenmesine ve kontrol programlarına katkıda bulunacağı
düşünülmüştür.

Keywords

A.centrale, A.marginale, cELISA, Nested-PCR, Adana

Investigation of Anaplasma marginale and Anaplasma centrale in Cattle in Adana Province by Serological and Molecular Methods

Abstract

Anaplasmosis is a common disease in tropical and
subtropical climate zone and is transmitted by vectors. Especially in
large cattle management systems, it has started to be detected frequently in
recent years. The aim of this study was to determine the prevalence
of Anaplasma spp. in cattle in Adana
province. For this aim, 187 blood samples were collected from cattle from fifteen
districts of Adana that have different climatic zones and examined by
Competitive ELISA (cELISA) and Nested-PCR methods. Seropositivity
was determined as 38.5% (72/187) in cattle. The molecular prevalence was
detected as 1.6% (3/187) for Anaplasma centrale
and 3.2% (6/187) for Anaplasma marginale
by Nested-Polymerase Chain Reaction (PCR) methods. In this study,
epidemiological data related to bovine anaplasmosis in Adana province of Turkey
were discussed in detail and it was thought that the obtained data would
contribute to disease prevention and control programs.

Keywords

A.centrale, A.marginale, cELISA, Nested-PCR, Adana

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