Controlled Release of Vitamin C from Chitosan Nanoparticles

Year 2018, Volume: 46 Issue: 1, 69 - 77, 01.03.2018

Aylin Altınışık Tagaç Önder Sarp Kadir Yurkakoç

Abstract

This work is consisted of two parts. The first was the synthesis and characterization of nanoparticles (ChNPs)
from Chitosan, a natural biopolymer. In the second part, preparation of Vitamin C loaded ChNPs and release
of vitamin C from the loaded nanoparticles were investigated. ChNPs were synthesized according to the ionic
gelation method and sodium tripolyphosphate (TPP) was used as the crosslinking agent. The particle size
distribution of the synthesized ChNPs was determined by using Zeta Sizer. Surface morphologies and crystal
structures of the nanoparticles were investigated by Scanning Electron Microscopy (SEM) and X- ray diffraction
(XRD) analysis, respectively. Structural analysis and thermal properties of ChNPs were also investigated by
Fournier Transform Infrared Spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Release
porofile of the Vitamin C loaded nanoparticles at same time were determined. As a result, average particle size
of the ChNPs was measured as 10 nm and loading efficiency of the ChNPs was calculated as 86% with very high
vitamin C concentration. Finally, the release mechanism of vitamin C from nanoparticles was determined to be
controlled by diffusion and swelling

Keywords

Chitosan nanoparticles, ionic gelletion, release kinetic, vitamin C.

References

• A. Altinisik, K. Yurdakoc, Chitosan/poly(vinyl alcohol) hydrogels for amoxicillin release, Polym. Bull., 71 (2014) 759-774.
• K.G.H. Desai, H. J. Park, Encapsulation of vitamin C in tripolyphosphate cross-linked chitosan microspheres by spray drying, J. Microencapsul., 22 (2005) 179- 192.
• M. Dogan, N. Ugurlu, F. Yulek, Ketorolac tromethamine loaded chitosan nanoparticles as a nanotherapeutic system for ocular diseases, J. Biol. Chem., 41 (2013) 81-86.
• L. Bugnicourt, L. Catherine, Interests of chitosan nanoparticles ionically cross-linked with tripolyphosphate for biomedical applications, Prog. Polym. Sci., 60 (2016) 1-17.
• K.A. Janes, P. Calvo, M.J. Alonso, Polysaccharide colloidal particles as delivery systems for macromolecules, Adv. Drug. Deliv. Rev., 47 (2001) 83- 97.
• M. Garcia-Fuentes, M.J. Alonso, Chitosan-based drug nanocarriers: where do we stand?, J. Control. Release., 161 (2012) 496-504.
• A. Grenha, Chitosan nanoparticles: a survey of preparation methods, J. Drug Target., 20 (2012) 291- 300.
• W. Fan, W. Yan, Z. Xu, H. Ni, Formation mechanism of monodisperse, low molecular weight chitosan nanoparticles by ionic gelation technique, Coll. Surf. B., 90 (2012) 21-27. 13. M. Hamidi, A. Azadi, H. Ashrafi, P. Rafiei, S. Mohamadi-samani, Taguchi orthogonal array design for the optimization of hydrogel nanoparticles for the intravenous delivery of small-molecule drugs, J. Appl. Polym. Sci., 126 (2012) 1714-1724. 14. E. Esposito, F. Cervellati, E. Menegatti, C. Nastruzzi, R. Cortesi, Spray dried Eudragit microparticles as encapsulation devices for vitamin C, Int. J. Pharm., 242 (2002) 329-334. 15. S.Y. Shiau, T.S. Hsu, Quantification of vitamin C requirement for juvenile hybrid tilapia, Oreochromisniloticus=Oreochromis aureus, with l-ascorbyl-2 monophosphate-Na and l-ascorbyl2-monophosphate-Mg, Aquaculture, 175 (1999) 317– 326.
• E.J. Jacobs, C.J. Connell, A.V. Patel, A. Chao, C. Rodriguez, J. Seymour, et al., Vitamin C and vitamin E supplement use and colorectal cancer mortality in a large American cancer society cohort, Cancer Epidemiol. Biomar. Prev,, 10 (2001) 17-23.
• G. Shklar, J.L. Schwartz, Ascorbic acid and cancer, Subcell. Biochem., 25 (1996) 233-247.
• H.M. Zhang, N. Wakisaka, Vitamin C inhibits the growth of a bacterial risk factor for gastric carcinoma: Helicobacter pylori, Cancer, 80 (1997) 1897-1903.
• A. Alishahi, et al., Shelf life and delivery enhancement of vitamin C using chitosan nanoparticles, Food Chem., 126 (2011) 935-940.
• M. Mietus-Snyder, M. J. Malloy, Endothelial dysfunction occurs in children with two genetic hyperlipidemias: improvement with antioxidant vitamin therapy, J. Pediatr., 133 (1998) 35-40.
• M. Jeserich, T. Schindler, M. Olschewski, M. Unmüssig, H. Just, U. Solzbach, Vitamin C improves endothelial function of epicardial coronary arteries in patients with hypercholesterolaemia or essential hypertension—assessed by cold pressor testing, Eur. Heart J., 20 (1999) 1676-1680.
• B. Mosinger, Higher cholesterol in human LDL is associated with the increase of oxidation susceptibility and the decrease of antioxidant defence: experimental and simulation data, BBA Mol. Bas., 1453 (1999) 180- 184.
• H. Ravi, V. Baskaran, Biodegradable chitosanglycolipid hybrid nanogels: A novel approach to encapsulate fucoxanthin for improved stability and bioavailability, Food Hydrocoll., 43 (2015) 717-725.
• S. Vimal, et al., Synthesis and characterization of CS/ TPP nanoparticles for oral delivery of gene in fish, Aquaculture., 358 (2012) 14-22.
• J. Gu, K. Al-Bayati, E.A. Ho, Development of antibodymodified chitosan nanoparticles for the targeted delivery of siRNA across the blood-brain barrier as a strategy for inhibiting HIV replication in astrocytes, Drug. Deliv. Transl. Res., (2017) 1-10.
• K. Santhi, et al., In-vitro Characterization of chitosan nanoparticles of fluconazole as a carrier for Sustained ocular delivery, J. Nanosci. Nanotechnol., 7 (2017) 41- 50.
• Q. Lifeng, et al. Preparation and antibacterial activity of chitosan nanoparticles, Carbohydr. Res., 339 (2004) 2693-2700.
• S. Mitra, et al. Tumour targeted delivery of encapsulated dextran–doxorubicin conjugate using chitosan nanoparticles as carrier, J. Control. Release., 74 (2001) 317-323.
• Y. Xu, Y. Du., Effect of molecular structure of chitosan on protein delivery properties of chitosan nanoparticles, Int. J. Pharm., 250 (2003) 215-226.
• K.A. Janes, et al., Chitosan nanoparticles as delivery systems for doxorubicin, J. Control. Release., 73 (2001): 255-267.
• Q. Gan, et al., Modulation of surface charge, particle size and morphological properties of chitosan–TPP nanoparticles intended for gene delivery, Colloid. Surf. B Biointer., 44 (2005) 65-73.
• Y. Pan, et al., Bioadhesive polysaccharide in protein delivery system: chitosan nanoparticles improve the intestinal absorption of insulin in vivo, Int. J. Pharm., 249 (2002) 139-147.
• S. Dash, et al., Kinetic modeling on drug release from controlled drug delivery systems, Acta Pol Pharm, 67 (2010) 217-23.
• R.W. Korsemeyer, R. Gurny, E.M. Doelker, P. Buri, N.A. Peppas, Mechanism of solute release from porous hydrophilic polymers, Int. J. Pharm., 15 (1983) 25-35