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A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.)

Year 2024, , 331 - 340, 04.02.2024
https://doi.org/10.18596/jotcsa.1310686

Abstract

Mangiferin is one of the antioxidants in Coffea arabica L. leaves that has many pharmacological effects. The content of secondary metabolites in the leaves including mangiferin can be affected by age. A Thin Layer Chromatography (TLC) method for the quantitative analysis of mangiferin in Coffea arabica L. leaves extract was developed and validated. The method was developed using a mobile phase prepared with analytical grade solvents: ethyl acetate, methanol, formic acid, and deionized water (8:2:1:1 v/v/v/v). Regression functions were established over the 199.98-600.00 ng/spot range with r=0.999. The limit of detection (LOD) and limit of quantification (LOQ) were 13.87 and 41.61 ng, respectively. The method was selective with a resolution value of more than 1.5 and specific with the spectra correlation value for purity and identity check of more than 0.99. The percentage RSD was found to be 2.43% for repeatability precision and 2.05% for intermediate precision. The method's accuracy was determined through the standard addition method by adding known quantities of standard mangiferin to the pre-analyzed test solution and the mean recovery was 101.69± 1,21%. This TLC Densitometry method was linear, sensitive, selective and specific, precise, accurate, and can be used for routine analysis of mangiferin. On the young Coffea arabica L. leaves, the concentration of mangiferin ± RSD was 0.830 ± 1.71% w/w, and on the old Coffea arabica L. leaves was 1.128± 1.59% w/w.

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References

  • 1. van der Vossen H, Bertrand B, Charrier A. Next generation variety development for sustainable production of arabica coffee (Coffea arabica L.): a review. Euphytica. 2015;204(2):243–56. Available from: <DOI>
  • 2. Jain PK, Kharya M, Gajbhiye A. Pharmacological evaluation of mangiferin herbosomes for antioxidant and hepatoprotection potential against ethanol induced hepatic damage. Drug Development and Industrial Pharmacy. 2013;39(11):1840–50. Available from:<DOI>
  • 3. He L, Peng X, Zhu J, Chen X, Liu H, Tang C, et al. Mangiferin attenuate sepsis-induced acute kidney injury via antioxidant and anti-inammatory effects. American Journal of Nephrology. 2014;40(5):441–50. Available from:<DOI>
  • 4. Wauthoz N, Balde A, Saïdou E, Marc B. Ethnopharmacology of Mangifera indica L . Bark and Pharmacological Studies of its Main C-Glucosylxanthone, Mangiferin. Journal of Pharmaceutical Sciences [Internet]. 2007;1(2):112–9. Available from:<URL>
  • 5. Márquez L, García-Bueno B, Madrigal JLM, Leza JC. Mangiferin decreases inflammation and oxidative damage in rat brain after stress. European Journal of Nutrition. 2012;51(6):729–39. Available from: <DOI>
  • 6. Niu Y, Liu J, Liu HY, Gao LH, Feng GH, Liu X, et al. Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity. Pharmaceutical Biology. 2016;54(9):1680–6. Available from:<DOI>
  • 7. Singh SK, Tiwari RM, Sinha SK, Danta CC, Prasad SK. Antimicrobial evaluation of mangiferin and its synthesized analogues. Asian Pacific Journal of Tropical Biomedicine [Internet]. 2012;2(2 SUPPL.):S884–7. Available from:<DOI>
  • 8. Rajendran P, Rengarajan T, Nandakumar N, Divya H, Nishigaki I. Mangiferin in cancer chemoprevention and treatment: Pharmacokinetics and molecular targets. Journal of Receptors and Signal Transduction. 2015;35(1):76–84. Available from: <DOI>
  • 9. Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, et al. Quantification and purification of mangiferin from Chinese mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H2O2-induced stress. International Journal of Molecular Sciences. 2012;13(9):11260–74. Available from:<DOI>
  • 10. Liu YW, Zhu X, Yang QQ, Lu Q, Wang JY, Li HP, et al. Suppression of methylglyoxal hyperactivity by mangiferin can prevent diabetes-associated cognitive decline in rats. Psychopharmacology (Berl). 2013;228(4):585–94. Available from: <DOI>
  • 11. Shinde K, Shinde V, Sharma K, Mahadik K. Phytochemical and Pharmacological Investigation on Vitex negundo Linn. Planta Medica. 2010;76(05). Available from:<DOI>
  • 12. Talamond P, Mondolot L, Gargadennec A, de Kochko A, Hamon S, Fruchier A, et al. First report on mangiferin (C-glucosyl-xanthone) isolated from leaves of a wild coffee plant, Coffea pseudozanguebariae (Rubiaceae). Acta Botanica Gallica. 2008;155(4):513–9. Available from:<DOI>
  • 13. Chi. Therapeutic Potential of the Natural Product Mangiferin in Metabolic Syndrome. The Journal of Nutrition. 2013;74–9. Available from: <DOI>
  • 14. Jyotshna, Srivastava P, Killadi B, Shanker K. Uni-dimensional double development HPTLC-densitometry method for simultaneous analysis of mangiferin and lupeol content in mango (Mangifera indica) pulp and peel during storage. Food Chemistry [Internet]. 2015;176:91–8. Available from: <DOI>
  • 15. Trevisan MTS, Farias de Almeida R, Soto G, De Melo Virginio Filho E, Ulrich CM, Owen RW. Quantitation by HPLC-UV of Mangiferin and Isomangiferin in Coffee (Coffea arabica) Leaves from Brazil and Costa Rica After Solvent Extraction and Infusion. Food Analytical Methods [Internet]. 2016;9(9):2649–55. Available from:<DOI>
  • 16. Marston A. Thin-layer chromatography with biological detection in phytochemistry. Journal of Chromatography A [Internet]. 2011;1218(19):2676–83. Available from:<DOI>
  • 17. Hahn-Deinstrop E. Applied Thin-Layer Chromatography : Best Practice and Avoidance of Mistakes Second, Revised and Enlarged Edition. WILEY-VCH Verlag GmbH dan Co. KGaA, Weinheim.; 2007. Available from:<URL>
  • 18. Dineshkumar B, Mitra A, Manjunatha M. Studies on the anti-diabetic and hypolipidemic potentials of mangiferin (xanthone glucoside) in streptozotocin-induced type 1 and type 2 diabetic model rats. The International Journal of Advanced Pharmaceutical Sciences and Research. 2010;1(1):75–85. Available from:<DOI>
  • 19. Retnaningtyas Y, Wulandari L, Wimala M. Inulin determination of yam bean tuber (Pacyrrhizus erosus l.) water extract from different altitude areas using TLC- Densitometry. Pharmaciana. 2022;12(1):21. Available from:<DOI>
  • 20. Sharma S, Goyal S, Chauhan K. A review on analytical method development and validation. International Journal of Applied Pharmaceutics. 2018;10(6):8–15 Available from:<DOI>
  • 21. Campanelli M. Chapter-2 Analytical Method Development and Validation Overview : 2020;2(May):123–40. Available from:<URL>
  • 22. Michael E.Swartz ISK. analitycal method development and validation. 2017. Available from:<DOI>
  • 23. AOAC International. Guidelines for Standard Method Performance Requirements. AOAC Official Methods of Analysis. 2016;9.
  • 24. Bhardwaj, P., Banarjee, A., Jindal, D., Kaur, C., Singh, G., Kumar, P., Sharma, A., & Kumar, R. . Validation of TLC-Densitometry Method for Estimation of Catechin in Acacia catechu Heartwood. Pharmaceutical Chemistry. 2020; 54(2): 184–189. Available from:<DOI>
  • 25. Abdelaleem EA, Abdelwahab NS. Stability-indicating TLC-densitometric method for simultaneous determination of paracetamol and chlorzoxazone and their toxic impurities. Journal of Chromatographic Science. 2013;51(2):187–91. Available from: <DOI>
  • 26. Sonia K, Shree BB, Lakshmi KS. HPTLC method development and validation: An overview. Journal of Pharmaceutical Sciences and Research. 2017;9(5):652–7. Available from : <URL>
  • 27. Campa C, Mondolot L, Rakotondravao A, Bidel LPR, Gargadennec A, Couturon E, et al. A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Annals of Botany. 2012;110(3):595–613. Available from : <DOI>.
Year 2024, , 331 - 340, 04.02.2024
https://doi.org/10.18596/jotcsa.1310686

Abstract

Project Number

-

References

  • 1. van der Vossen H, Bertrand B, Charrier A. Next generation variety development for sustainable production of arabica coffee (Coffea arabica L.): a review. Euphytica. 2015;204(2):243–56. Available from: <DOI>
  • 2. Jain PK, Kharya M, Gajbhiye A. Pharmacological evaluation of mangiferin herbosomes for antioxidant and hepatoprotection potential against ethanol induced hepatic damage. Drug Development and Industrial Pharmacy. 2013;39(11):1840–50. Available from:<DOI>
  • 3. He L, Peng X, Zhu J, Chen X, Liu H, Tang C, et al. Mangiferin attenuate sepsis-induced acute kidney injury via antioxidant and anti-inammatory effects. American Journal of Nephrology. 2014;40(5):441–50. Available from:<DOI>
  • 4. Wauthoz N, Balde A, Saïdou E, Marc B. Ethnopharmacology of Mangifera indica L . Bark and Pharmacological Studies of its Main C-Glucosylxanthone, Mangiferin. Journal of Pharmaceutical Sciences [Internet]. 2007;1(2):112–9. Available from:<URL>
  • 5. Márquez L, García-Bueno B, Madrigal JLM, Leza JC. Mangiferin decreases inflammation and oxidative damage in rat brain after stress. European Journal of Nutrition. 2012;51(6):729–39. Available from: <DOI>
  • 6. Niu Y, Liu J, Liu HY, Gao LH, Feng GH, Liu X, et al. Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity. Pharmaceutical Biology. 2016;54(9):1680–6. Available from:<DOI>
  • 7. Singh SK, Tiwari RM, Sinha SK, Danta CC, Prasad SK. Antimicrobial evaluation of mangiferin and its synthesized analogues. Asian Pacific Journal of Tropical Biomedicine [Internet]. 2012;2(2 SUPPL.):S884–7. Available from:<DOI>
  • 8. Rajendran P, Rengarajan T, Nandakumar N, Divya H, Nishigaki I. Mangiferin in cancer chemoprevention and treatment: Pharmacokinetics and molecular targets. Journal of Receptors and Signal Transduction. 2015;35(1):76–84. Available from: <DOI>
  • 9. Luo F, Lv Q, Zhao Y, Hu G, Huang G, Zhang J, et al. Quantification and purification of mangiferin from Chinese mango (Mangifera indica L.) cultivars and its protective effect on human umbilical vein endothelial cells under H2O2-induced stress. International Journal of Molecular Sciences. 2012;13(9):11260–74. Available from:<DOI>
  • 10. Liu YW, Zhu X, Yang QQ, Lu Q, Wang JY, Li HP, et al. Suppression of methylglyoxal hyperactivity by mangiferin can prevent diabetes-associated cognitive decline in rats. Psychopharmacology (Berl). 2013;228(4):585–94. Available from: <DOI>
  • 11. Shinde K, Shinde V, Sharma K, Mahadik K. Phytochemical and Pharmacological Investigation on Vitex negundo Linn. Planta Medica. 2010;76(05). Available from:<DOI>
  • 12. Talamond P, Mondolot L, Gargadennec A, de Kochko A, Hamon S, Fruchier A, et al. First report on mangiferin (C-glucosyl-xanthone) isolated from leaves of a wild coffee plant, Coffea pseudozanguebariae (Rubiaceae). Acta Botanica Gallica. 2008;155(4):513–9. Available from:<DOI>
  • 13. Chi. Therapeutic Potential of the Natural Product Mangiferin in Metabolic Syndrome. The Journal of Nutrition. 2013;74–9. Available from: <DOI>
  • 14. Jyotshna, Srivastava P, Killadi B, Shanker K. Uni-dimensional double development HPTLC-densitometry method for simultaneous analysis of mangiferin and lupeol content in mango (Mangifera indica) pulp and peel during storage. Food Chemistry [Internet]. 2015;176:91–8. Available from: <DOI>
  • 15. Trevisan MTS, Farias de Almeida R, Soto G, De Melo Virginio Filho E, Ulrich CM, Owen RW. Quantitation by HPLC-UV of Mangiferin and Isomangiferin in Coffee (Coffea arabica) Leaves from Brazil and Costa Rica After Solvent Extraction and Infusion. Food Analytical Methods [Internet]. 2016;9(9):2649–55. Available from:<DOI>
  • 16. Marston A. Thin-layer chromatography with biological detection in phytochemistry. Journal of Chromatography A [Internet]. 2011;1218(19):2676–83. Available from:<DOI>
  • 17. Hahn-Deinstrop E. Applied Thin-Layer Chromatography : Best Practice and Avoidance of Mistakes Second, Revised and Enlarged Edition. WILEY-VCH Verlag GmbH dan Co. KGaA, Weinheim.; 2007. Available from:<URL>
  • 18. Dineshkumar B, Mitra A, Manjunatha M. Studies on the anti-diabetic and hypolipidemic potentials of mangiferin (xanthone glucoside) in streptozotocin-induced type 1 and type 2 diabetic model rats. The International Journal of Advanced Pharmaceutical Sciences and Research. 2010;1(1):75–85. Available from:<DOI>
  • 19. Retnaningtyas Y, Wulandari L, Wimala M. Inulin determination of yam bean tuber (Pacyrrhizus erosus l.) water extract from different altitude areas using TLC- Densitometry. Pharmaciana. 2022;12(1):21. Available from:<DOI>
  • 20. Sharma S, Goyal S, Chauhan K. A review on analytical method development and validation. International Journal of Applied Pharmaceutics. 2018;10(6):8–15 Available from:<DOI>
  • 21. Campanelli M. Chapter-2 Analytical Method Development and Validation Overview : 2020;2(May):123–40. Available from:<URL>
  • 22. Michael E.Swartz ISK. analitycal method development and validation. 2017. Available from:<DOI>
  • 23. AOAC International. Guidelines for Standard Method Performance Requirements. AOAC Official Methods of Analysis. 2016;9.
  • 24. Bhardwaj, P., Banarjee, A., Jindal, D., Kaur, C., Singh, G., Kumar, P., Sharma, A., & Kumar, R. . Validation of TLC-Densitometry Method for Estimation of Catechin in Acacia catechu Heartwood. Pharmaceutical Chemistry. 2020; 54(2): 184–189. Available from:<DOI>
  • 25. Abdelaleem EA, Abdelwahab NS. Stability-indicating TLC-densitometric method for simultaneous determination of paracetamol and chlorzoxazone and their toxic impurities. Journal of Chromatographic Science. 2013;51(2):187–91. Available from: <DOI>
  • 26. Sonia K, Shree BB, Lakshmi KS. HPTLC method development and validation: An overview. Journal of Pharmaceutical Sciences and Research. 2017;9(5):652–7. Available from : <URL>
  • 27. Campa C, Mondolot L, Rakotondravao A, Bidel LPR, Gargadennec A, Couturon E, et al. A survey of mangiferin and hydroxycinnamic acid ester accumulation in coffee (Coffea) leaves: biological implications and uses. Annals of Botany. 2012;110(3):595–613. Available from : <DOI>.
There are 27 citations in total.

Details

Primary Language English
Subjects Physical Chemistry (Other)
Journal Section RESEARCH ARTICLES
Authors

Yuni Retnaningtyas 0000-0003-0725-9560

Nora Putri Narindra 0009-0009-1806-230X

Nia Kristiningrum 0000-0002-0796-9741

Project Number -
Publication Date February 4, 2024
Submission Date June 7, 2023
Acceptance Date November 22, 2023
Published in Issue Year 2024

Cite

Vancouver Retnaningtyas Y, Putri Narindra N, Kristiningrum N. A Densitometric Method for Determination of Mangiferin, an Antioxidant Compound, with Thin Layer Chromatography in the Leaf Extracts of Coffee (Coffea Arabica L.). JOTCSA. 2024;11(1):331-40.