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Evaluation of Changes Biological Activity of Onosma Sericeum Willd (Boraginaceae) Based on Collection Time and Extraction Solvent, and Determination of Its Mineral and Trace Element Composition

Year 2019, , 355 - 364, 20.10.2019
https://doi.org/10.18596/jotcsa.585036

Abstract

In this study total
phenol, flavonoid, and free radical scavenging activity of Onosma Sericeum
Willd (Boraginaceae), grown in Turkey and used for traditional wound healing
were determined using
spectrophotometric methods. Its cytotoxic effect on
breast (MCF-7) cancer cells was investigated. Furthermore, mineral and trace
element composition of the plant roots, collected in different times, was
determined using ınductively coupled plasma mass spectroscopy (ICP-MS). Onosma
sericeum Willd, collected in Besni, Adıyaman region in May, June and July, and
dried properly, were extracted by using different solvents in the study. Total
phenol content of the extracts was calculated using the Folin-Ciocalteu method
in gallic acid equivalent as 58, 42±0, 13-93, 34±0, 68 mg (GAE)/g. The
flavonoid content was calculated as 21.79±1, 3-60, 33±0, 36 mg (KUE)/g in
quercetin equivalent by using the aluminum chloride method. Free radical
scavenging activity was measured through a spectrophotometer according to
2,2-diphenyl-1- picrylhydrazyl (DPPH) assay. DPPH radical scavenging activity
rates were found to be in the range of 60.27±0.06-78.60±0.21% in the form of %
inhibition. It was observed that extracts prepared by using ethanol produced
cytotoxic effect on the MCF-7 cancer cells depending on the dosage. It was
determined that the rates of phenol, flavonoid, free radical scavenging
activity and cytotoxic effect differed based on the solvent used for extraction
and the time of plant collection. Moreover, trace element and mineral composition
of the plant samples collected in different times were determined. 

Thanks

The authors thank Assoc. Prof. Dr. Rıza Binzet from the Department of Biology at the Faculty of Arts and Sciences, Mersin University for the botanical identification of the plant. We also thank the Department of Biostatistics and Medical Informatics, Faculty of Medicine, Mersin University and Asena Ayça Özdemir, Specialist, for their contributions in performing statistical analyses.

References

  • 1. Vukic MD, Vukovic NL, Djelic GT, Popovic S, Zaric MM, Baskic DD, Krstic GB, Tesevic VV, Kacaniova MM. Antibacterial and cytotoxic activities of naphthoquinone pigments from Onosma Visianii Clem. EXCLI Journal. 2017;16:73-88.
  • 2. Ozgen U, Ikbal M, Hacimuftuoglu A, Houghton P.J, Gocer F, Dogan H, Coskun M. Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots. J Ethnopharmacol. 2006;104:100–3.
  • 3. Binzet R,Orcan N. A New species of Onosma (Boraginaceae) from Southern Turkey. Missouri Botanical Garden Press.2007;17(1): 8-10.
  • 4. Akçin ÖE, Aktaş T, Altıntaş MY. Myosotis Alpestris F.W.Schmidt (Boraginaceae) türünün anatomik özellikleri. Ordu Univ. J. Sci. Tech. 2013;3(1): 61-8.
  • 5. Tosun A, Akkol EK, Bahadır Ö, Yesilada E. Evaluation of anti-inflammatory and antinociceptive activities of some Onosma L. Species growing in Turkey.J Ethnopharmacol.2008;120:378–81.
  • 6. Ozgen U, Coskun, M, Kazaz, C, Secen, H. Naphthoquinones from the roots of Onosma argentatumHub.-Mor (Boraginaceae). Turk J Chem. 2004;28: 451–4.
  • 7. Ozgen U, Houghton, PJ, Ogundipe, Y, Coskun, M. The Antioxidant and antimicrobial activities of Onosma argentatum and Rubiaperegrina. Fitoterapia. 2003;74: 682–5.
  • 8. Sharma S,Khan, K, Sultana, S. Effect of Onosma echiodes on DMBA/croton oil mediated carvcinogenic response, hyperproliferation and oxidative damage in murine skin. Life Sci. 2004;75(20): 2391–410.
  • 9. Ozer MS, Kirkan B, Sarikurkcu C, Cengiz M, Ceylan O, Atılgan N, Tepe B. Onosmaheterophyllum:Phenolic composition, enzyme inhibitory and antioxidant activities. Ind Crops Prod. 2018;111:179–84.
  • 10. Sezik E, Yeşilada E, Tabata M, Honda G, Takaishi Y, Fujita T, Tanaka T, Takedau Y. Traditional medicine in Turkey VIII. Folk medicine in East Anatolia; Erzurum, Erzincan, Ağrı, Kars, Igdır Provinces. Econ. Bot. 1997;51(3):195-211.
  • 11. Gharehmatrossian S, Popov Y, Ghorbanli M, Safaeian S, Iranbakhsh A. Phytochemical and morphological evidences for Shikonin production by plant cell cultures of OnosmasericeumWilld. Braz. Arch. Biol. Technol.2016; 59: 1-7.
  • 12. Dziri S, Hassen I, Fatnassi S, Mrabet Y, Casabianca H, Hanchi B. Phenolicconstituents, antioxidant and antimicrobial activities of rosygarlic (Alliumroseum var. odoratissimum). J Funct Foods. 2012; 4(2): 423-32.
  • 13. Baye H, Hymete A. Lead and cadmium accumulation in medicinal plants collected from environmentally different sites. BullEnviron Contam Toxicol. 2010; 84(2): 197-201.
  • 14. Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V. Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. FoodChem. 2012; 135(2): 494-501.
  • 15. Slinkard K. Singleton VL. Total phenols analysis: Automation and comparison with manual methods.Am. J. Enol. Vitic, 1977;28: 49 – 55.
  • 16. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums, Food Chem. 2003;81(3): 321 – 6.
  • 17. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compost Anal.2011;24: 1043–8.
  • 18. Turan I, Demir S, Aliyazıcıoğlu R, Aliyazıcıoğlu Y. Evaluation of antioxidant and cytotoxic properties of Primula vulgaris Leaf Extract. KSU J. Nat. Sci. 2017; 20(4): 361-7.
  • 19. Cadirci E, Suleyman H, Aksoy H, Halici Z, Ozgen U, Koc A. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats. Chem Biol Interact. 2007;170(1):40-8.
  • 20. Binzet R, Akçin ÖE. The anatomical properties of two Onosma L. (Boraginaceae) species from Turkey.J Med Plant Res. 2012; 6(17): 3288-94.
  • 21. Papageorgiou VP, Assimopoulou AN, Ballis AC. Alkannins and shikonins: a new class of wound healing agents. Curr Med Chem. 2008;15:3248-67.
  • 22. Wang L, Li F, Liu X, Chen B, Yu K, Wang MK. Meroterpenoids and a naphthoquinone from Arnebiaeuchroma and their cytotoxic activity. Planta Med.2015;81:320-6.
  • 23. Naz S, Ahmad S, AjazRasool S, AsadSayeed S, SiddiqIR. Antibacterial activity directed isolation of compounds from Onosma hispidum. MicrobiolRes.2006;161:43-8.
  • 24. Mazandarani M, Moghaddam PZ, Baiat H, Zolfaghari MR, Ghaemiand EA, Hemati H. Antioxidant activity, phenol, flavonoid and anthocyanin contents in various extracts of Onosma dichroanthum Boiss. in north of Iran Masoumeh. Iran. J. Plant Physiol. 2011; 1(3): 169 – 76.
  • 25. Kirkan B, Sarikurkcu C, Ozer MS, Cengiz M, Atılgan N,Ceylan O, Tepe B. Phenolic profile, antioxidant and enzyme inhibitory potential of Onosma tauricum var. Tauricum. Ind Crops Prod.2018; 125:549–55.
  • 26. Bıçkıcı Ş. Bazı Alkanna türlerinin biyoaktivite rehberli izolasyon yöntemi kullanılarak çeşitli kanser hücreleri üzerindeki sitotoksik etkilerinin in vitro ortamda taranması [Master of Science Thesis].[İzmir]:Ege University;2006.
Year 2019, , 355 - 364, 20.10.2019
https://doi.org/10.18596/jotcsa.585036

Abstract

References

  • 1. Vukic MD, Vukovic NL, Djelic GT, Popovic S, Zaric MM, Baskic DD, Krstic GB, Tesevic VV, Kacaniova MM. Antibacterial and cytotoxic activities of naphthoquinone pigments from Onosma Visianii Clem. EXCLI Journal. 2017;16:73-88.
  • 2. Ozgen U, Ikbal M, Hacimuftuoglu A, Houghton P.J, Gocer F, Dogan H, Coskun M. Fibroblast growth stimulation by extracts and compounds of Onosma argentatum roots. J Ethnopharmacol. 2006;104:100–3.
  • 3. Binzet R,Orcan N. A New species of Onosma (Boraginaceae) from Southern Turkey. Missouri Botanical Garden Press.2007;17(1): 8-10.
  • 4. Akçin ÖE, Aktaş T, Altıntaş MY. Myosotis Alpestris F.W.Schmidt (Boraginaceae) türünün anatomik özellikleri. Ordu Univ. J. Sci. Tech. 2013;3(1): 61-8.
  • 5. Tosun A, Akkol EK, Bahadır Ö, Yesilada E. Evaluation of anti-inflammatory and antinociceptive activities of some Onosma L. Species growing in Turkey.J Ethnopharmacol.2008;120:378–81.
  • 6. Ozgen U, Coskun, M, Kazaz, C, Secen, H. Naphthoquinones from the roots of Onosma argentatumHub.-Mor (Boraginaceae). Turk J Chem. 2004;28: 451–4.
  • 7. Ozgen U, Houghton, PJ, Ogundipe, Y, Coskun, M. The Antioxidant and antimicrobial activities of Onosma argentatum and Rubiaperegrina. Fitoterapia. 2003;74: 682–5.
  • 8. Sharma S,Khan, K, Sultana, S. Effect of Onosma echiodes on DMBA/croton oil mediated carvcinogenic response, hyperproliferation and oxidative damage in murine skin. Life Sci. 2004;75(20): 2391–410.
  • 9. Ozer MS, Kirkan B, Sarikurkcu C, Cengiz M, Ceylan O, Atılgan N, Tepe B. Onosmaheterophyllum:Phenolic composition, enzyme inhibitory and antioxidant activities. Ind Crops Prod. 2018;111:179–84.
  • 10. Sezik E, Yeşilada E, Tabata M, Honda G, Takaishi Y, Fujita T, Tanaka T, Takedau Y. Traditional medicine in Turkey VIII. Folk medicine in East Anatolia; Erzurum, Erzincan, Ağrı, Kars, Igdır Provinces. Econ. Bot. 1997;51(3):195-211.
  • 11. Gharehmatrossian S, Popov Y, Ghorbanli M, Safaeian S, Iranbakhsh A. Phytochemical and morphological evidences for Shikonin production by plant cell cultures of OnosmasericeumWilld. Braz. Arch. Biol. Technol.2016; 59: 1-7.
  • 12. Dziri S, Hassen I, Fatnassi S, Mrabet Y, Casabianca H, Hanchi B. Phenolicconstituents, antioxidant and antimicrobial activities of rosygarlic (Alliumroseum var. odoratissimum). J Funct Foods. 2012; 4(2): 423-32.
  • 13. Baye H, Hymete A. Lead and cadmium accumulation in medicinal plants collected from environmentally different sites. BullEnviron Contam Toxicol. 2010; 84(2): 197-201.
  • 14. Pytlakowska K, Kita A, Janoska P, Połowniak M, Kozik V. Multi-element analysis of mineral and trace elements in medicinal herbs and their infusions. FoodChem. 2012; 135(2): 494-501.
  • 15. Slinkard K. Singleton VL. Total phenols analysis: Automation and comparison with manual methods.Am. J. Enol. Vitic, 1977;28: 49 – 55.
  • 16. Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums, Food Chem. 2003;81(3): 321 – 6.
  • 17. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compost Anal.2011;24: 1043–8.
  • 18. Turan I, Demir S, Aliyazıcıoğlu R, Aliyazıcıoğlu Y. Evaluation of antioxidant and cytotoxic properties of Primula vulgaris Leaf Extract. KSU J. Nat. Sci. 2017; 20(4): 361-7.
  • 19. Cadirci E, Suleyman H, Aksoy H, Halici Z, Ozgen U, Koc A. Effects of Onosma armeniacum root extract on ethanol-induced oxidative stress in stomach tissue of rats. Chem Biol Interact. 2007;170(1):40-8.
  • 20. Binzet R, Akçin ÖE. The anatomical properties of two Onosma L. (Boraginaceae) species from Turkey.J Med Plant Res. 2012; 6(17): 3288-94.
  • 21. Papageorgiou VP, Assimopoulou AN, Ballis AC. Alkannins and shikonins: a new class of wound healing agents. Curr Med Chem. 2008;15:3248-67.
  • 22. Wang L, Li F, Liu X, Chen B, Yu K, Wang MK. Meroterpenoids and a naphthoquinone from Arnebiaeuchroma and their cytotoxic activity. Planta Med.2015;81:320-6.
  • 23. Naz S, Ahmad S, AjazRasool S, AsadSayeed S, SiddiqIR. Antibacterial activity directed isolation of compounds from Onosma hispidum. MicrobiolRes.2006;161:43-8.
  • 24. Mazandarani M, Moghaddam PZ, Baiat H, Zolfaghari MR, Ghaemiand EA, Hemati H. Antioxidant activity, phenol, flavonoid and anthocyanin contents in various extracts of Onosma dichroanthum Boiss. in north of Iran Masoumeh. Iran. J. Plant Physiol. 2011; 1(3): 169 – 76.
  • 25. Kirkan B, Sarikurkcu C, Ozer MS, Cengiz M, Atılgan N,Ceylan O, Tepe B. Phenolic profile, antioxidant and enzyme inhibitory potential of Onosma tauricum var. Tauricum. Ind Crops Prod.2018; 125:549–55.
  • 26. Bıçkıcı Ş. Bazı Alkanna türlerinin biyoaktivite rehberli izolasyon yöntemi kullanılarak çeşitli kanser hücreleri üzerindeki sitotoksik etkilerinin in vitro ortamda taranması [Master of Science Thesis].[İzmir]:Ege University;2006.
There are 26 citations in total.

Details

Primary Language English
Subjects Analytical Chemistry
Journal Section Articles
Authors

Selda Doğan 0000-0003-2589-8585

Mustafa Gündoğan This is me 0000-0002-5063-6301

Publication Date October 20, 2019
Submission Date July 1, 2019
Acceptance Date August 27, 2019
Published in Issue Year 2019

Cite

Vancouver Doğan S, Gündoğan M. Evaluation of Changes Biological Activity of Onosma Sericeum Willd (Boraginaceae) Based on Collection Time and Extraction Solvent, and Determination of Its Mineral and Trace Element Composition. JOTCSA. 2019;6(3):355-64.