Biosynthesis and Characterization of Co3O4NPs Utilizing Prickly Pear Fruit Extract and its Biological Activities

Year 2022, , 1117 - 1128, 30.11.2022

Ms. D. Nagajothi J Maheswari

https://doi.org/10.18596/jotcsa.993633

Abstract

In the current research, there is a low level of research and information about the interaction of cobalt oxide nanoparticles (Co3O4NPs) in biological systems. This research creates a very simple and cost-effective preparation of cobalt oxide nanoparticles by using prickly pear fruit extract as a reducing agent, which may be further used for biological applications like antimicrobial, antioxidant, DNA interaction and in-vitro anticancer activity. The use of prickly pear fruit extract acts as a good reducing agent and is responsible for easy preparation and reducing the toxicity of cobalt oxide nanoparticles. The fabricated biogenic nanoparticles were confirmed by microscopic and spectroscopic analytical techniques like Ultra Violet-Visible spectrometer, Fourier transforms infrared spectrometer (FTIR), X-ray Diffraction Method (XRD), Energy-dispersive X-ray spectroscopy (EDS), Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM). The average size of the synthesized nanoparticles is 36.24 nm. In the MTT assay, the prepared cobalt oxide NPs haspotential mechanisms of cytotoxicity and in-vitro anticancer activity in Hepatocellular carcinoma cancer cells (HepG2). The microbial activities like antibacterial and antifungal studies of the biosynthesized nanoparticles were performed by the Disc method. The Co3O4NPs with DNA interaction were examined by UV-Visible and fluorescence spectroscopic methods. The binding constant value of biogenic Co3O4NPs with CT-DNA was observed by UV-Visible spectroscopy with a result of 2.57x105mol-1. The binding parameters and quenching constants were observed by fluorescence spectroscopic methods having values of Ksv=7.1x103, kq=7.1x108, Ka=3.47.1x105, n=0.9119. From the findings, Co3O4NPs may be utilized as a medicinal aid for their antibacterial, antifungal, antioxidant, DNA binding and in-vitro anticancer activities.

Keywords

Cobalt oxide nanoparticles, Prickly Pear, antibacterial, antifungal, antioxidant, DNA binding, in vitro anticancer activity

Supporting Institution

Ayya Nadar Janaki Ammal College, Affiliated to Maduarai Kamaraj University, Madurai, Tamil Nadu, India

Thanks

We thank our college for giving research aids.

References

• 1. Gibbs RD. Comparative chemistry and phylogeny of flowering plants. In Royal Society of Canada; 1954.
• 2. Esa YAM, Sapawe N. A short review on biosynthesis of cobalt metal nanoparticles. Materials Today: Proceedings. 2020;31:378–85.
• 3. Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. 3rd ed. London ; New York: Chapman and Hall; 1998. 302 p. ISBN :978-0-412-57260-9.
• 4. Trease G, Evans W. Textbook of pharmacognosy. 14th edition. London; 1989.
• 5. Vijayakumar S. Eco-friendly synthesis of gold nanoparticles using fruit extracts and in vitro anticancer studies. Journal of Saudi Chemical Society. 2019 Sep;23(6):753–61.
• 6. Diallo A, Beye AC, Doyle TB, Park E, Maaza M. Green synthesis of Co 3 O 4 nanoparticles via Aspalathus linearis : Physical properties. Green Chemistry Letters and Reviews. 2015 Oct 2;8(3–4):30–6.
• 7. Sivachidambaram M, Vijaya JJ, Kaviyarasu K, Kennedy LJ, Al-Lohedan HA, Jothi Ramalingam R. A novel synthesis protocol for Co 3 O 4 nanocatalysts and their catalytic applications. RSC Adv. 2017;7(62):38861–70. 8. Sharma JK, Srivastava P, Singh G, Akhtar MS, Ameen S. Green synthesis of Co3O4 nanoparticles and their applications in thermal decomposition of ammonium perchlorate and dye-sensitized solar cells. Materials Science and Engineering: B. 2015 Mar;193:181–8.
• 9. Dubey S, Kumar J, Kumar A, Sharma YC. Facile and green synthesis of highly dispersed cobalt oxide (Co3O4) nano powder: Characterization and screening of its eco-toxicity. Advanced Powder Technology. 2018 Nov;29(11):2583–90.
• 10. Mulya Dewi NO, Yulizar Y, Bagus Apriandanu DO. Green synthesis of Co 3 O 4 nanoparticles using Euphorbia heterophylla L. leaves extract: characterization and photocatalytic activity. IOP Conf Ser: Mater Sci Eng. 2019 May 3;509:012105.
• 11. Han L, Yang DP, Liu A. Leaf-templated synthesis of 3D hierarchical porous cobalt oxide nanostructure as direct electrochemical biosensing interface with enhanced electrocatalysis. Biosensors and Bioelectronics. 2015 Jan;63:145–52.
• 12. Anuradha CT, Raji P. Effect of annealing temperature on antibacterial, antifungal and structural properties of bio-synthesized Co 3 O 4 nanoparticles using Hibiscus Rosa-sinensis. Mater Res Express. 2019 Jul 17;6(9):095063.
• 13. Saeed M, Akram N, Atta-ul-Haq, Naqvi SAR, Usman M, Abbas MA, et al. Green and eco-friendly synthesis of Co3O4 and Ag-Co3O4: Characterization and photo-catalytic activity. Green Processing and Synthesis. 2019 Jan 28;8(1):382–90.
• 14. Ikhuoria EU, Omorogbe SO, Sone BT, Maaza M. Bioinspired shape controlled antiferromagnetic Co3O4 with prism like-anchored octahedron morphology: A facile green synthesis using Manihot esculenta Crantz extract. Science and Technology of Materials. 2018 May;30(2):92–8.
• 15. Matinise N, Mayedwa N, Fuku XG, Mongwaketsi N, Maaza M. Green synthesis of cobalt (II, III) oxide nanoparticles using Moringa Oleifera natural extract as high electrochemical electrode for supercapacitors. In Stellenbosch, South Africa; 2018 [cited 2022 Oct 3]. p. 040005.
• 16. Sebeia N, Jabli M, Ghith A. Biological synthesis of copper nanoparticles, using Nerium oleander leaves extract: Characterization and study of their interaction with organic dyes. Inorganic Chemistry Communications. 2019 Jul;105:36–46.
• 17. Ullah M, Naz A, Mahmood T, Siddiq M, Bano A. Biochemical synthesis of nickel & cobalt oxide nano-particles by using biomass waste. International Journal of Enhanced Research in Science Technology & Engineering. 2014;3:415–22.
• 18. Bibi I, Nazar N, Iqbal M, Kamal S, Nawaz H, Nouren S, et al. Green and eco-friendly synthesis of cobalt-oxide nanoparticle: Characterization and photo-catalytic activity. Advanced Powder Technology. 2017 Sep;28(9):2035–43.
• 19. Saravanakumar P, Muthukumar M, Muthuchudarkodi R, Ramkumar P. Piper nigrum mediated green synthesis, characterization of undoped cobalt oxide and cerium ion doped cobalt oxide nanoparticles. Int J Recent Res Aspects. 2018;918–23.
• 20. Khalil AT, Ovais M, Ullah I, Ali M, Shinwari ZK, Maaza M. Physical properties, biological applications and biocompatibility studies on biosynthesized single phase cobalt oxide (Co3O4) nanoparticles via Sageretia thea (Osbeck.). Arabian Journal of Chemistry. 2020 Jan;13(1):606–19.
• 21. Das RK, Golder AK. Co3O4 spinel nanoparticles decorated graphite electrode: Bio-mediated synthesis and electrochemical H2O2 sensing. Electrochimica Acta. 2017 Oct;251:415–26.
• 22. Mindru I, Gingasu D, Patron L, Ianculescu A, Surdu VA, Culita DC, et al. A new approach: Synthesis of cobalt aluminate nanoparticles using tamarind fruit extract. Materials Science and Engineering: B. 2019 Jul;246:42–8.
• 23. Rasheed T, Nabeel F, Bilal M, Iqbal HMN. Biogenic synthesis and characterization of cobalt oxide nanoparticles for catalytic reduction of direct yellow-142 and methyl orange dyes. Biocatalysis and Agricultural Biotechnology. 2019 May;19:101154.
• 24. Majeed S, Abdullah MS bin, Nanda A, Ansari MT. In vitro study of the antibacterial and anticancer activities of silver nanoparticles synthesized from Penicillium brevicompactum (MTCC-1999). Journal of Taibah University for Science. 2016 Oct;10(4):614–20.
• 25. Khan S, Ansari AA, Khan AA, Ahmad R, Al-Obaid O, Al-Kattan W. In vitro evaluation of anticancer and antibacterial activities of cobalt oxide nanoparticles. J Biol Inorg Chem. 2015 Dec;20(8):1319–26.
• 26. Stankic S, Suman S, Haque F, Vidic J. Pure and multi metal oxide nanoparticles: synthesis, antibacterial and cytotoxic properties. J Nanobiotechnol. 2016 Dec;14(1):73.
• 27. Hafeez M, Shaheen R, Akram B, Zain-ul-Abdin, Haq S, Mahsud S, et al. Green synthesis of cobalt oxide nanoparticles for potential biological applications. Mater Res Express. 2020 Feb 1;7(2):025019.
• 28. Bhushan M, Kumar Y, Periyasamy L, Viswanath AK. Antibacterial applications of α-Fe2O3/Co3O4 nanocomposites and study of their structural, optical, magnetic and cytotoxic characteristics. Appl Nanosci. 2018 Feb;8(1–2):137–53.
• 29. Rajeswari VD, Khalifa AS, Elfasakhany A, Badruddin IA, Kamangar S, Brindhadevi K. Green and ecofriendly synthesis of cobalt oxide nanoparticles using Phoenix dactylifera L: antimicrobial and photocatalytic activity. Appl Nanosci [Internet]. 2021 Aug 30 [cited 2022 Oct 3];
• 30. Mauro M, Crosera M, Pelin M, Florio C, Bellomo F, Adami G, et al. Cobalt Oxide Nanoparticles: Behavior towards Intact and Impaired Human Skin and Keratinocytes Toxicity. IJERPH. 2015 Jul 17;12(7):8263–80.
• 31. Kamaraj M, Nithya TG, Santhosh P, Mulugeta K. Rapid Green Synthesis of Silver Nanoparticles Using Ethiopian Cactus Pear Fruit Peel Infusions and Evaluation of Its In Vitro Clinical Potentials. J Inorg Organomet Polym. 2020 Sep;30(9):3832–6.
• 32. El-Mostafa K, El Kharrassi Y, Badreddine A, Andreoletti P, Vamecq J, El Kebbaj M, et al. Nopal Cactus (Opuntia ficus-indica) as a Source of Bioactive Compounds for Nutrition, Health and Disease. Molecules. 2014 Sep 17;19(9):14879–901.
• 33. Livrea MA, Tesoriere L. Health benefits and bioactive components of the fruits from Opuntia ficus-indica [L.] Mill. Journal of the Professional Association for cactus Development. 2006;8(1):73–90.
• 34. Badri A, Slimi S, Guergueb M, Kahri H, Mateos X. Green synthesis of copper oxide nanoparticles using Prickly Pear peel fruit extract: Characterization and catalytic activity. Inorganic Chemistry Communications. 2021 Dec;134:109027.
• 35. Muñoz-Carrillo MG, Garcidueñas-Piña C, Valerio-García RC, Carrazco-Rosales JL, Morales-Domínguez JF. Green Synthesis of Silver Nanoparticles from the Opuntia ficus-indica Fruit and Its Activity against Treated Wastewater Microorganisms. Chauhan BPS, editor. Journal of Nanomaterials. 2020 Nov 17;2020:1–10.
• 36. Pooja S, Vidyasagar G. Biosynthesis of Silver Nanoparticles from Three Opuntia Sps. Int J Adv Sci Res Manag. 2019;4:1–11.
• 37. Sankarganesh M, Dhaveethu Raja J, Sakthikumar K, Solomon RV, Rajesh J, Athimoolam S, et al. New bio-sensitive and biologically active single crystal of pyrimidine scaffold ligand and its gold and platinum complexes: DFT, antimicrobial, antioxidant, DNA interaction, molecular docking with DNA/BSA and anticancer studies. Bioorganic Chemistry. 2018 Dec;81:144–56.
• 38. Sankarganesh M, Adwin Jose P, Dhaveethu Raja J, Kesavan MP, Vadivel M, Rajesh J, et al. New pyrimidine based ligand capped gold and platinum nano particles: Synthesis, characterization, antimicrobial, antioxidant, DNA interaction and in vitro anticancer activities. Journal of Photochemistry and Photobiology B: Biology. 2017 Nov;176:44–53.
• 39. Sakthikumar K, Dhaveethu Raja J, Vijay Solomon R, Sankarganesh M. Density functional theory molecular modelling, DNA interactions, antioxidant, antimicrobial, anticancer and biothermodynamic studies of bioactive water soluble mixed ligand complexes. Journal of Biomolecular Structure and Dynamics. 2019 Jul 3;37(10):2498–514.
• 40. Vadivel M, Sankarganesh M, Raja JD, Rajesh J, Mohanasundaram D, Alagar M. Bioactive constituents and bio-waste derived chitosan / xylan based biodegradable hybrid nanocomposite for sensitive detection of fish freshness. Food Packaging and Shelf Life. 2019 Dec;22:100384.
• 41. Jeyaraj M, Praphakar RA, Rajendran C, Ponnamma D, Sadasivuni KK, Munusamy MA, et al. Surface functionalization of natural lignin isolated from Aloe barbadensis Miller biomass by atom transfer radical polymerization for enhanced anticancer efficacy. RSC Adv. 2016;6(56):51310–9.
• 42. Madasamy S, Sundan S, Krishnasamy L. Preparation Of Cold Cream Against Clinical Pathogen Using Caralluma Adscendens Var. Attenuata. Asian J Pharm Clin Res. 2020 Jun 29;120–3.
• 43. Bibi I, Nazar N, Iqbal M, Kamal S, Bhatti HN, Nouren S, et al. Corrigendum to ‘Green and eco-friendly synthesis of cobalt-oxide nanoparticle: Characterization and photo-catalytic activity’ [Adv. Powder Technol. 28 (2017) 2035–2043]. Advanced Powder Technology. 2017 Oct;28(10):2796.
• 44. Iravani S, Varma RS. Sustainable synthesis of cobalt and cobalt oxide nanoparticles and their catalytic and biomedical applications. Green Chem. 2020;22(9):2643–61.
• 45. Rajasekar K, Muthukumaravel K, Ashok K, Babu M. Phytosynthesis of copper oxide nanoparticles from Opuntia ficus and its antibreast cancer activity against MCF-7 cell line (invasive ductal carcinoma). Malaya J Matematik S (2). 2020;4414–9.
• 46. Dubey S, Kumar J, Kumar A, Sharma YC. Facile and green synthesis of highly dispersed cobalt oxide (Co3O4) nano powder: Characterization and screening of its eco-toxicity. Advanced Powder Technology. 2018 Nov;29(11):2583–90.
• 47. Salavati-Niasari M, Davar F, Mazaheri M, Shaterian M. Preparation of cobalt nanoparticles from [bis(salicylidene)cobalt(II)]–oleylamine complex by thermal decomposition. Journal of Magnetism and Magnetic Materials. 2008 Feb;320(3–4):575–8. 48. Herrero M, Benito P, Labajos F, Rives V. Nanosize cobalt oxide-containing catalysts obtained through microwave-assisted methods. Catalysis Today. 2007 Oct 30;128(3–4):129–37.
• 49. Manteghi F, Kazemi SH, Peyvandipour M, Asghari A. Preparation and application of cobalt oxide nanostructures as electrode materials for electrochemical supercapacitors. RSC Adv. 2015;5(93):76458–63.
• 50. Bhushan M, Kumar Y, Periyasamy L, Viswanath AK. Antibacterial applications of α-Fe2O3/Co3O4 nanocomposites and study of their structural, optical, magnetic and cytotoxic characteristics. Appl Nanosci. 2018 Feb;8(1–2):137–53.
• 51. Moradpoor H, Safaei M, Rezaei F, Golshah A, Jamshidy L, Hatam R, et al. Optimisation of Cobalt Oxide Nanoparticles Synthesis as Bactericidal Agents. Open Access Maced J Med Sci. 2019 Aug 30;7(17):2757–62.
• 52. Wang Y, Aker WG, Hwang H min, Yedjou CG, Yu H, Tchounwou PB. A study of the mechanism of in vitro cytotoxicity of metal oxide nanoparticles using catfish primary hepatocytes and human HepG2 cells. Science of The Total Environment. 2011 Oct;409(22):4753–62.
• 53. Ajarem JS, Maodaa SN, Allam AA, Taher MM, Khalaf M. Benign Synthesis of Cobalt Oxide Nanoparticles Containing Red Algae Extract: Antioxidant, Antimicrobial, Anticancer, and Anticoagulant Activity. J Clust Sci. 2022 Mar;33(2):717–28.
• 54. Abbasi BA, Iqbal J, Mahmood T, Qyyum A, Kanwal S. Biofabrication of iron oxide nanoparticles by leaf extract of Rhamnus virgata : Characterization and evaluation of cytotoxic, antimicrobial and antioxidant potentials. Appl Organometal Chem [Internet]. 2019 Jul [cited 2022 Oct 4];33(7).
• 55. Mianai RS, Ghasemzadeh MA, Monfared MRZ. Green Fabrication of Cobalt NPs using Aqueous Extract of Antioxidant Rich Zingiber and Their Catalytic Applications for the Synthesis of Pyrano[2,3-c]pyrazoles. CCHTS. 2019 May 3;22(1):18–26.
• 56. Chattopadhyay S, Dash SK, Tripathy S, Das B, Kar Mahapatra S, Pramanik P, et al. Cobalt oxide nanoparticles induced oxidative stress linked to activation of TNF-α/caspase-8/p38-MAPK signaling in human leukemia cells: Anticancer activity of CoO nanoparticles in vitro and in vivo. J Appl Toxicol. 2015 Jun;35(6):603–13.
• 57. Abamor ES. Antileishmanial activities of caffeic acid phenethyl ester loaded PLGA nanoparticles against Leishmania infantum promastigotes and amastigotes in vitro. Asian Pacific Journal of Tropical Medicine. 2017 Jan;10(1):25–34.
• 58. Ebrahiminezhad A, Zare-Hoseinabadi A, Sarmah AK, Taghizadeh S, Ghasemi Y, Berenjian A. Plant-Mediated Synthesis and Applications of Iron Nanoparticles. Mol Biotechnol. 2018 Feb;60(2):154–68.
• 59. Matinise N, Fuku XG, Kaviyarasu K, Mayedwa N, Maaza M. ZnO nanoparticles via Moringa oleifera green synthesis: Physical properties & mechanism of formation. Applied Surface Science. 2017 Jun;406:339–47.
• 60. Jin C, Fu T, Wang R, Liu H, Zou J, Zhao Z, et al. Fluorinated molecular beacons as functional DNA nanomolecules for cellular imaging. Chem Sci. 2017;8(10):7082–6.
• 61. Morris DL. DNA-bound metal ions: recent developments. Biomolecular Concepts. 2014 Oct 1;5(5):397–407.
• 62. Yang D, Hartman MR, Derrien TL, Hamada S, An D, Yancey KG, et al. DNA Materials: Bridging Nanotechnology and Biotechnology. Acc Chem Res. 2014 Jun 17;47(6):1902–11.
• 63. Sun H, Ren J, Qu X. Carbon Nanomaterials and DNA: from Molecular Recognition to Applications. Acc Chem Res. 2016 Mar 15;49(3):461–70.
• 64. Liu B, Liu J. Methods for preparing DNA-functionalized gold nanoparticles, a key reagent of bioanalytical chemistry. Anal Methods. 2017;9(18):2633–43.
• 65. Izatt RM, Christensen JJ, Rytting JH. Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and and nucleotides. Chem Rev. 1971 Oct;71(5):439–81.
• 66. Pershina AG, Sazonov AE, Filimonov VD. Magnetic nanoparticles–DNA interactions: design and applications of nanobiohybrid systems. Russ Chem Rev. 2014 Apr 30;83(4):299–322.
• 67. Chaurasia M, Tomar D, Chandra S. Synthesis, spectroscopic characterization and DNA binding studies of Cu(II) complex of Schiff base containing benzothiazole moiety. Journal of Taibah University for Science. 2019 Dec 11;13(1):1050–9.
• 68. Thulasiram B, devi CS, Kumar YP, Aerva RR, Satyanarayana S, Nagababu P. Correlation Between Molecular Modelling and Spectroscopic Techniques in Investigation With DNA Binding Interaction of Ruthenium(II) Complexes. J Fluoresc. 2017 Mar;27(2):587–94.
• 69. Chaurasia M, Tomar D, Chandra S. Synthesis, spectral characterization, and DNA binding studies of Co(II), Ni(II), Cu(II) and Zn(II) complexes of Schiff base 2-((1H-1,2,4-triazol-3-ylimino)methyl)-5-methoxyphenol. Journal of Molecular Structure. 2019 Mar;1179:431–42.
• 70. Park SY, Lytton-Jean AKR, Lee B, Weigand S, Schatz GC, Mirkin CA. DNA-programmable nanoparticle crystallization. Nature. 2008 Jan;451(7178):553–6.
• 71. Shaban NZ, Aboelsaad AM, Shoueir KR, Abdulmalek SA, Awad D, Shaban SY, et al. Chitosan-based dithiophenolato nanoparticles: Preparation, mechanistic information of DNA binding, antibacterial and cytotoxic activities. Journal of Molecular Liquids. 2020 Nov;318:114252.
• 72. Sankarganesh M, Adwin Jose P, Dhaveethu Raja J, Kesavan MP, Vadivel M, Rajesh J, et al. New pyrimidine based ligand capped gold and platinum nano particles: Synthesis, characterization, antimicrobial, antioxidant, DNA interaction and in vitro anticancer activities. Journal of Photochemistry and Photobiology B: Biology. 2017 Nov;176:44–53.
• 73. Han MS, Lytton-Jean AKR, Oh BK, Heo J, Mirkin CA. Colorimetric Screening of DNA-Binding Molecules with Gold Nanoparticle Probes. Angew Chem Int Ed. 2006 Mar 6;45(11):1807–10.
• 74. Mirzaei-Kalar Z, Yavari A, Jouyban A. Increasing DNA binding affinity of doxorubicin by loading on Fe3O4 nanoparticles: A multi-spectroscopic study. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2020 Mar;229:117985.
• 75. Zhang NN, Bigdeli F, Miao Q, Hu ML, Morsali A. Ultrasonic-assisted synthesis, characterization and DNA binding studies of Ru(II) complexes with the chelating N-donor ligand and preparing of RuO2 nanoparticles by the easy method of calcination. Journal of Organometallic Chemistry. 2018 Dec;878:11–8.
• 76. Hafeez M, Shaheen R, Akram B, Zain-ul-Abdin, Haq S, Mahsud S, et al. Green synthesis of cobalt oxide nanoparticles for potential biological applications. Mater Res Express. 2020 Feb 1;7(2):025019.