Antioxidant, antimicrobial and anticarcinogenic activities of Sambucus ebulus L. flowers, fruits and leaves

Year 2014, Volume: 18 Issue: 1, 22 - 25, 15.02.2014

Zehra Meriç Leyla Bitiş Seher Birteksöz Tan Suna Özbaş Turan Jülide Akbuga

Abstract

ABSTRACT: The aim of this study was to evaluate the antioxidant, antimicrobial and anticarsinogenic
activities of methanol extract obtained from flowers, fruits and leaves of Sambucus
ebulus L. Free radical scavenging activities and total phenolic contents of the species
were assayed by DPPH method and Folin–Ciocalteu method, respectively. The antimicrobial
activity of the extracts were tested by the micro-broth dilution method against seven microbial
species. L929 (ATCC, CCL-1) mouce fibroblast cell line was used for the determiation of
in vitro citotoxicity activity and HeLa (ATCC, CCl-2) human cervics adenocarcinoma cell line
was used for the determination of anticarcinogenic and growth inhibition activity. Leaves of
Sambucus ebulus L showed the strongest antioxidant activity and also leaves of plant has
highest phenolic content, Sambucus ebulus L. exracts were found to have no significant
activity against any strains of bacteria. Only Sambucus ebulus fruit extract has shown moderate
effect against Candida albicans. In 10μg/mL concentration the highest anticarcinogenic
activity were found in leaves extract, in addition that none of the extracts were not found
cytotoxic.
KEY WORDS: Sambucus ebulus, antioxidant, antimicrobial, anticarsinogenic, cytotoxic

Keywords

Sambucus ebulus, antioxidant, antimicrobial, anticarsinogenic, cytotoxic

Antioxidant, antimicrobial and anticarcinogenic activities of Sambucus ebulus L. flowers, fruits and leaves

Abstract

Bu çalışmanın amacı Türkiye’de yetişen Sambucus ebulus L. bitkisinin çiçek, meyve ve yapraklarından hazırlanan metanol ekstrelerinin antioksidan, antimikrobiyal ve antikarsinojenik aktivitelerini değerlendirmektir. Ekstrelerin serbest radikal süpürücü aktivite tayinleri DPPH metodu ile, toplam fenolik madde miktar tayini ise Folin–Ciocalteu metoduyla, antimikrobiyal aktivitesi 7 mikroorganizma türüne karşı mikro dilüsyon yöntemiyle, antikarsinojenik aktivite tayini ise L929 (ATCC, CCL-1) fare fibroblast hücre hattı ve HeLa (ATCC, CCl-2) kanser hücre hattı kullanılarak MTT yöntemiyle sitotoksik etkinlikleri araştırmak üzere test edildi. DPPH radikal süpürücü aktivite tayininde en yüksek aktiviteyi yaprak ekstresi gösterdi ayrıca toplam fenolik madde miktarı da en yüksek oranda bu ekstrede bulundu. Antimikrobiyal aktivite tayininde sadece meyve ekstresi Candida albicans’a karşı orta derecede etki göstermiştir. Antikarsinojenik aktivite test sonuçlarına göre ise yaprak ekstresi en yüksek değeri göstermiş, hiçbir ekstre sitotoksik bulunmamıştır.

Keywords

Sambucus ebulus, antioksidan, antimikrobiyal, antikarsinojenik, sitotoksik1: Flowers, 2: Fruits, 3: LeavesFIGURE 1. Cell viability and growth inhibition

References

• Klancnik A, Piskernik S, Jersek B, Mozina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods 2010; 81:121-6.
• Barbour EK, Sharif MA, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004; 93: 1-7.
• Sgouras D, Duncan R. Methods for the Evaluation of Biocompatibility of Soluble Synthetic Polymer Which have potential for Biomedical Use: 1-Use of the Tetrazolium- Based Colorimetric assay (MTT) As a Preliminary Screen for Evaluation of In Vitro Cytotoxicity. J Mater Sci Mater Med 1990; 1: 61-8.
• Mossman T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
• Beekman AC, Barentsen AR, Woerdenbag HJ, Uden WV, Pras N. Stereochemistry-dependent cytotoxicity of some artemisinin derivatives. J Nat Prod 1997; 60: 325-30.
• Davis PH. Flora of Turkey and the East Aegean Islands. vol.4, Edinburgh, Edinburgh University Pres,1978; 541-543.
• Krawitz C, Mraheil MA, Stein M, Imirzalioglu C, Domann E, Pleschka S, Hain T. Inhibitory activity of a standardized elderberry liquid extract against clinicallyrelevant human respiratory bacterial pathogens and influenza A and B viruses. BMC Complement Altern Med 2011; 11-16.
• Blumenthal M. The Complete German Commission E Monographs, American Botanical Council, Boston, 1998; 124.
• WHO Monographs on Selected Medicinal Plants, World Health Organization, Geneva, Vol. 1, 1999; 269-275.
• Genç GE, Özhatay N. An ethnobotanical study in Çatalca (European Part of İstanbul) II, Turkish J Pharm Sci 2006; 3:73Koçyiğit M Özhatay N. Wild Plants Used as Medicinal Purpose in Yalova (Northwest Turkey), Turkish J Pharm Sci 2006; 3:91-103.
• Kültür Ş. Medicinal plants used in Kırklareli Province (Turkey). J Ethnopharmacol 2007; 111:341–64.
• Tuzlacı E, Tolon E.Turkish folk medicinal plants, part III: Şile (İstanbul). Fitoterapia 2000; 71:673-85.
• Tuzlacı E. ‘Şifa Niyetine’ Türkiye’nin Bitkisel Halk İlaçları. Alfa Basım Yayım Dağıtım Ltd. Şti. İstanbul. 2006; 315-9.
• Ozsoy N, Can A, Yanardag R, Akev N. Antioxidant activity of Smilax excelsa L. leaf extracts. Food Chem 2008; 110:571-83.
• Gao X, Ohlander M, Jeppssen N, Björk L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea Buckthorn (Hippophae rhamnoides L.) during maturation. J Agric Food Chem 2000; 48:1485-90.
• Clinical and Laboratory Standards Institute (CLSI). Reference Method for Broth Dilution Antifungal Susceptbility Testing of Yeasts; Approved Standart M27-A CLSI, Wayne, PA, USA. 2002.
• Clinical and Laboratory Standards Institute (CLSI). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically - Seventh Edition: Approved Standard M7-A7. CLSI, Wayne, PA, USA. 200