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Aloe Vera Bitkisinden Yeşil Sentez Yöntemiyle Elde Edilen Gümüş Nanopartiküllerin Antimikrobiyal ve Antibiyofilm Etkilerinin Araştırılması

Year 2024, Volume: 7 Issue: 1, 166 - 176, 22.01.2024
https://doi.org/10.47495/okufbed.1279010

Abstract

Küresel olarak antibiyotiklerin yanlış kullanımı, dirençli bakteri suşlarının gelişmesine neden olmuştur. Aloe vera ekstraktından yeşil sentez ile elde edilen gümüş nanopartiküllerin Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii ve Staphylococcus aureus'a karşı antimikrobiyal ve antibiyofilm etkileri araştırıldı. Aloe vera bitkisinden gümüş nanopartiküllerin sentezi yeşil sentez yöntemi ile gerçekleştirilmiştir. Gümüş nanopartiküllerin karakterizasyonu UV-Vis Spektroskopi, FT-IR, SEM, TEM, XRD ile değerlendirildikten sonra patojenik mikroorganizmalar üzerindeki antimikrobiyal etkisi mikrodilüsyon yöntemi ile değerlendirilmiştir. Gümüş nanopartiküllerin Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii ve Staphylococcus aureus üzerindeki Minimum İnhibitör Konsantrasyon (MIC) değerleri (µg/mL) sırasıyla 32, 64, 32 ve 32 olarak bulundu. Bu çalışmada, biyofilm inhibisyon oranı kristal viyole yöntemi ile değerlendirilmiştir. Yeşil sentez gümüş nanopartiküller bakteriler üzerinde antibiyofilm etki göstermiştir.

References

  • Ahmad B., Chang L., Satti UQ., Rehman SU., Arshad H., Mustafa G., Shaukat U., Wang F., Tong C. Phyto-synthesis, characterization, and in vitro antibacterial activity of silver nanoparticles using various plant extracts. Bioengineering 2022; 9(12): 779.
  • Ali SG., Ansari MA., Alzohairy MA., Alomary MN., Jalal M., AlYahya S., Asiri SMM., Khan HM. Effect of biosynthesized ZnO nanoparticles on multi-drug resistant Pseudomonas aeruginosa. Antibiotics 2020; 9(5): 260.
  • Arsene MM., Viktorovna PI., Sergei GV., Hajjar F., Vyacheslavovna YN., Vladimirovna ZA., Sachivkina, N. Phytochemical analysis, antibacterial and antibiofilm activities of Aloe vera aqueous extract against selected resistant gram-negative bacteria Involved in Urinary Tract Infections. Fermentation 2022; 8(11): 626.
  • Bilgili B., Karademir F., Bozaci E., Özdoğan E., Ayhan H., Ayhan F. Liquidambar orientalis mill. leaf aqueous extract for the synthesis of silver nanoparticles and immobilization on textile fabrics for biomedical applications. Textile and Apparel 2016; 26(4): 421-429.
  • Baran MF. Prunus avium kiraz yaprağı özütü ile gümüş nanopartikül (AgNP) sentezi ve antimikrobiyal etkisinin incelenmesi. Dicle Üniversitesi Mühendislik Fakültesi Mühendislik Dergisi 2019; 10(1): 221-227.
  • Ellis DH., Maurer-Gardner EI., Sulentic CE., Hussain SM. Silver nanoparticle antibacterial efficacy and resistance development in key bacterial species. Biomedical Physics & Engineering Express 2018; 5(1): 015013.
  • Fan Q., Zhang Y., Yang H., Wu Q., Shi C., Zhang C., Xia X., Wang X. Effect of coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes. Food Control 2018; 90: 274–281.
  • Gecer EN., Erenler R., Temiz C., Genc N., Yildiz I. Green synthesis of silver nanoparticles from Echinacea purpurea (L.) Moench with antioxidant profile. Particulate Science and Technology 2022; 40(1): 50-57.
  • Hatipoğlu A. Gümüş nanopartiküllerin yeşil sentezi ve bazı gıda patojenleri üzerindeki antimikrobiyal etkileri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2022; 26(1): 106-114.
  • Huang Q., Zhu X. Rapid and large-scale synthesis of pitaya-like silver nanostructures as highly efficient surface-enhanced Raman scattering substrates. Talanta, 2013; 105: 117-123.

Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis

Year 2024, Volume: 7 Issue: 1, 166 - 176, 22.01.2024
https://doi.org/10.47495/okufbed.1279010

Abstract

Misuse of antibiotics globally has resulted in the development of resistant bacterial strains. Antimicrobial and antibiofilm effects of silver nanoparticles obtained by green synthesis from Aloe vera extract against Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii and Staphylococcus aureus were investigated. The synthesis of silver nanoparticles from the aloe vera plant was carried out by the green synthesis method. After the characterization of silver nanoparticles was evaluated by UV-Vis Spectroscopy, FT-IR, SEM, TEM, XRD its antimicrobial effect on pathogenic microorganisms was evaluated by microdilution method. The Minimum Inhibitor Concentration (MIC) values (µg/mL) of silver nanoparticles on Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and Staphylococcus aureus were found to be 32, 64, 32 and 32, respectively. In this study, the biofilm inhibition rate was evaluated by the crystal violet method. Green synthesis silver nanoparticles showed antibiofilm effect on bacteria.

References

  • Ahmad B., Chang L., Satti UQ., Rehman SU., Arshad H., Mustafa G., Shaukat U., Wang F., Tong C. Phyto-synthesis, characterization, and in vitro antibacterial activity of silver nanoparticles using various plant extracts. Bioengineering 2022; 9(12): 779.
  • Ali SG., Ansari MA., Alzohairy MA., Alomary MN., Jalal M., AlYahya S., Asiri SMM., Khan HM. Effect of biosynthesized ZnO nanoparticles on multi-drug resistant Pseudomonas aeruginosa. Antibiotics 2020; 9(5): 260.
  • Arsene MM., Viktorovna PI., Sergei GV., Hajjar F., Vyacheslavovna YN., Vladimirovna ZA., Sachivkina, N. Phytochemical analysis, antibacterial and antibiofilm activities of Aloe vera aqueous extract against selected resistant gram-negative bacteria Involved in Urinary Tract Infections. Fermentation 2022; 8(11): 626.
  • Bilgili B., Karademir F., Bozaci E., Özdoğan E., Ayhan H., Ayhan F. Liquidambar orientalis mill. leaf aqueous extract for the synthesis of silver nanoparticles and immobilization on textile fabrics for biomedical applications. Textile and Apparel 2016; 26(4): 421-429.
  • Baran MF. Prunus avium kiraz yaprağı özütü ile gümüş nanopartikül (AgNP) sentezi ve antimikrobiyal etkisinin incelenmesi. Dicle Üniversitesi Mühendislik Fakültesi Mühendislik Dergisi 2019; 10(1): 221-227.
  • Ellis DH., Maurer-Gardner EI., Sulentic CE., Hussain SM. Silver nanoparticle antibacterial efficacy and resistance development in key bacterial species. Biomedical Physics & Engineering Express 2018; 5(1): 015013.
  • Fan Q., Zhang Y., Yang H., Wu Q., Shi C., Zhang C., Xia X., Wang X. Effect of coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes. Food Control 2018; 90: 274–281.
  • Gecer EN., Erenler R., Temiz C., Genc N., Yildiz I. Green synthesis of silver nanoparticles from Echinacea purpurea (L.) Moench with antioxidant profile. Particulate Science and Technology 2022; 40(1): 50-57.
  • Hatipoğlu A. Gümüş nanopartiküllerin yeşil sentezi ve bazı gıda patojenleri üzerindeki antimikrobiyal etkileri. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2022; 26(1): 106-114.
  • Huang Q., Zhu X. Rapid and large-scale synthesis of pitaya-like silver nanostructures as highly efficient surface-enhanced Raman scattering substrates. Talanta, 2013; 105: 117-123.
There are 10 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section RESEARCH ARTICLES
Authors

Aybek Yiğit 0000-0001-8279-5908

Ayşe Karacalı Tunç 0000-0002-6453-9887

Büşra Merve Sarıtaş 0000-0001-8955-4699

Publication Date January 22, 2024
Submission Date April 7, 2023
Acceptance Date August 26, 2023
Published in Issue Year 2024 Volume: 7 Issue: 1

Cite

APA Yiğit, A., Karacalı Tunç, A., & Sarıtaş, B. M. (2024). Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 7(1), 166-176. https://doi.org/10.47495/okufbed.1279010
AMA Yiğit A, Karacalı Tunç A, Sarıtaş BM. Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis. Osmaniye Korkut Ata University Journal of Natural and Applied Sciences. January 2024;7(1):166-176. doi:10.47495/okufbed.1279010
Chicago Yiğit, Aybek, Ayşe Karacalı Tunç, and Büşra Merve Sarıtaş. “Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7, no. 1 (January 2024): 166-76. https://doi.org/10.47495/okufbed.1279010.
EndNote Yiğit A, Karacalı Tunç A, Sarıtaş BM (January 1, 2024) Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7 1 166–176.
IEEE A. Yiğit, A. Karacalı Tunç, and B. M. Sarıtaş, “Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis”, Osmaniye Korkut Ata University Journal of Natural and Applied Sciences, vol. 7, no. 1, pp. 166–176, 2024, doi: 10.47495/okufbed.1279010.
ISNAD Yiğit, Aybek et al. “Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi 7/1 (January 2024), 166-176. https://doi.org/10.47495/okufbed.1279010.
JAMA Yiğit A, Karacalı Tunç A, Sarıtaş BM. Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis. Osmaniye Korkut Ata University Journal of Natural and Applied Sciences. 2024;7:166–176.
MLA Yiğit, Aybek et al. “Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis”. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 7, no. 1, 2024, pp. 166-7, doi:10.47495/okufbed.1279010.
Vancouver Yiğit A, Karacalı Tunç A, Sarıtaş BM. Investigation of The Antimicrobial and Antibiofilm Effects of Silver Nanoparticles Obtained From Aloe Vera Plant by Green Synthesis. Osmaniye Korkut Ata University Journal of Natural and Applied Sciences. 2024;7(1):166-7.

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