Development and Validation of HPLC-UV Method for Determination of Meloxicam in Tablet Dosage Formulation

Year 2023, Volume: 3 Issue: 3, 59 - 63, 31.07.2023

Reyhan Sıla Çelik Burak Bayrak Yücel Kadıoğlu

Abstract

Objective: The development and validation of a novel, simple, and quick high-performance liquid chromatography– ultra viole t detection (HPLC-UV) technique for measuring meloxicam in pharmaceutical formulations was made.

Methods: The technique parameters were tuned to be 0.8 mL/min flow rate, variable column temperature, 290 nm wavelength, 10 μL injection volume, and a mobile phase combination of water (with 0.6% triflour acetic acid—pH:2.6) and methanol (30 : 70 v/v) to carry out this study. In this study, valsartan was used as ınternal standard (IS).

Results: Specificity, the limit of quantitation (LOQ), linearity, accuracy, precision, stability, recovery, and ruggedness were all tested. The technique was linear between 1.0 μg/mL and 50 μg/mL, with precision (relative standard d (RSD) %) and accuracy (relative error %) of less than 3.9% and 0.7%, respectively. The LOQ and LOD values of method were 1.00 and 0.25 μg/mL, respectively. Analytical recovery from pharmaceutical preparations was performed according to the standard addition method, and the average analytical recovery value was determined as 100.4%. The developed and validated HPLC-UV method was successfully applied to 4 commercial tablet dosage formulations obtained from a local pharmacy store in Turkey (Zeloxim, Melox, Meksun, Exen).

Conclusion: It has been concluded that the developed HPLC-UV method is sensitive, accurate, and precise and can be successfully applied in quality control studies in the pharmaceutical industry.

Keywords

HPLC-UV, meloxicam, tablet dosage formulation

Ethical Statement

Since this study is an in vitro (quantification in pharmaceutical preparations) study, ethics committee approval is not required.

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