Effects of Salt Stress and Inoculation Ratios in Cell Cultures of Rubia tinctorum L.

Year 2017, , 328 - 334, 01.06.2017

Pınar Nartop Şeref Akay Aynur Gürel

https://doi.org/10.16984/saufenbilder.283920

Cited By: 1

Abstract

In this study, salt stress was implemented to callus
and cell suspension cultures of Rubia
tinctorum L. at 100 mM and 200 mM concentrations and the effects on fresh
and dry weights (g), relative dry weight ratios, and alizarin and purpurin
contents (mg/g dry weight) of biomass were determined. Due to an increase in
salt concentrations, biomass obtained from callus and cell suspension cultures
were decreased. In callus cultures, due to the low viability and proliferation
rate of the callus cells, the biomass showed no significant increase. Alizarin
and purpurin contents decreased as the salt concentrations in callus cultures
increased, but amounts of these compounds increased in cell suspension
cultures. Inoculation ratios were also found effective on fresh and dry
weights, and alizarin and purpurin contents of the samples tested. 1:2
inoculation ratio caused higher alizarin and purpurin contents in cell
suspension cultures, but biomass amounts were found higher at 1:4 inoculation
ratio. The highest
alizarin (3.183 mg/g) and
purpurin (1.153 mg/g) contents
were detected in the sample that had 1:2 inoculation ratios and was obtained
from the medium at 200 mM NaCl concentration.

Keywords

alizarin, callus, cell suspension, purpurin, Rubia tinctorum L., salt stress

Rubia tinctorum L. Hücre Kültürlerinde Tuz Stresi ve Inokülasyon Oranlarının Etkileri

Abstract

 Bu çalışmada, Rubia tinctorum L. callus ve hücre
süspansiyon kültürlerine 100 mM ve 200 mM konsantrasyonlarda tuz stresi
uygulanarak elde edilen biyokütlelerdeki yaş ve kuru ağırlıklar (g), bağıl kuru
ağırlık oranları (mg/g kuru ağırlık) ve alizarin ve purpurin içerikleri üzerine
etkileri belirlenmiştir. Tuz consantrasyonundaki artışa bağlı olarak, callus ve
hücre süspansiyon kültürlerinden elde edilen biyokütle azalmıştır. Kallus
kültürlerindeki hücrelerde düşük canlılık ve çoğalma oranına rağmen, biyokütle
belirgin bir düşüş göstermemiştir. Kallus kültürlerinde tuz konsantrasyonu
arttıkça alizarin ve purpurin içeriği azalmıştır, ancak bu bileşiklerin miktarı
hücre süspansiyon kültürlerinde artmıştır. Yapılan denemelerde inokülasyon
oranlarının yaş ve kuru ağırlık miktarı ve alizarin ve purpurin içeriği üzerine
etkili olduğu tespit edilmiştir. Hücre süspansiyon kültürlerinde 1:2
inokülasyon oranı daha yüksek alizarin ve purpurin içeriği sağlarken, 1:4
inoküsyon oranında biyokütle miktarı daha fazladır. En yüksek alizarin (3.183 mg/g) ve purpurin (1.153 mg/g) içerikleri 1:2 inokülasyon oranında ve 200 mM
NaCl içeren besin ortamından tespit edilmiştir.

Keywords

alizarin, callus, cell suspension, purpurin, Rubia tinctorum L., salt stress

References

• [1] A.G. Ercan, K.M. Taşkın, K. Turgut and Yüce S, “Agrobacterium rhizogenes-mediated hairy root formation in some Rubia tinctorum L. populations grown in Turkey”, Turk. J. Bot., vol. 2, pp. 373-377, 1999.
• [2] P. Banyai, N. Kuzovkina, L. Kursinszki and E. Szoke, “HPLC analysis of alizarin and purpurin produced by Rubia tinctorum L. hairy root cultures”, Chromatographia, vol. 63, pp. 211-214, 2006.
• [3] H. Baydar and T. Karadoğan, “Agronomic potential and industrial value of madder (Rubia tinctorum L.) as a dye crop”, Turk. J. Agric. For., vol. 30, pp. 287-293, 2006.
• [4] S.U. Park, Y.K. Kim, S.Y. Lee, “Establishment of hairy root culture of Rubia akane Nakai for alizarin and purpurin production”, Sci. Res. Essays., vol. 4(2), pp. 94-97, 2009.
• [5] A.H. Lodhi and B.V. Charlwood, “Agrobacterium rhizogenes-mediated transformation of Rubia peregrina L.: In vitro accumulation of anthraquinones”, Plant Cell. Tiss. Org., vol. 46(2), pp. 103-108, 1996.
• [6] Y. Doğan, S. Başlar, H.H. Mert and G. Ay, “Plants used as natural dye sources in Turkey”, Econ. Bot., vol. 57(4), pp. 442-453, 2003.
• [7] D. Kákoniová, S. Vaverková, D. Lišková, E. Urgeová, Z. Juráková, “The possibility to enhance flavonoids production in Rubia tinctorum L. callus cultures”, Nova Biotechnologica, vol. 9-2, pp. 191-197, 2009.
• [8] M. Perassolo, A.M. Giulietti and J.R. Talou, “Study of glutamate addition on secondary metabolism of Rubia tinctorum cell suspension cultures”, J. Biotechnol., vol. 131, pp. 43-47, 2007a.
• [9] M. Perassolo, C. Quevedo, V. Busto, F. Ianone, A.M. Giulietti and J.R. Talou, “Enhance of anthraquinone production by effect of proline and aminoindan-2-phosphonic acid in Rubia tinctorum suspension cultures”, Enzyme Microb. Tech., vol.41, pp.181-185, 2007b.
• [10] V. Busto, A. M. Giulietti and J.R. Talou, “Hydrodynamic stress induces anthraquinone production in Rubia tinctorum cell suspension cultures”, J. Biotechnol., vol.131, pp.43-47, 2007.
• [11] N. Orban, I. Boldizsar, Z. Scucs and B. Danos, “Influence of different elicitors on the synthesis of anthraquinone derivatives in Rubia tinctorum L. cell suspension cultures”, Dyes and Pigments, vol. 77, pp.249-257, 2008.
• [12] T. Murashige and F. Skoog, “A revised medium for rapid growth and bio-assays with tobacco tissue cultures”, Physiol. Plantarum, vol.15, pp.473-497, 1962.
• [13] S.L. Maki, M. Delgado and W. Adelberg, “Time course study of ancymidol for micropropagation of Hosta in a liquid culture system”, Hort. Science, vol.40(3), pp.764-766, 2005.
• [14] E.A. Hussein, E.M. Aqlan, “Effect of mannitol and sodium chloride on some total secondary metabolites fenugreek calli cultured in vitro”, Plant Tissue Cult. & Biotech., vol. 21(1), pp. 35-43, 2011.
• [15] N.M. Nazif, M.R. Rady and M.M. Seif El-Nasr, “Stimulation of anthraquinone production in suspension cultures of Cassia acutifolia by salt stress”, Fitoterapia, vol.71, pp. 34-40, 2000.