Mikroskop görüntüsünde otomatik embriyonik kök hücre tespiti ve sayımı

Year 2016, Volume: 20 Issue: 2, 83 - 97, 01.08.2016

Gökçen Çetinel Ali Furkan Kamanlı

https://doi.org/10.16984/saufenbilder.23719

Abstract

Mikroskop altında hücre sayımı, bir uzman tarafından mikroskop merceğine sürekli bakılarak veya otomatik hücre sayımı yöntemleri kullanılarak yapılabilmektedir. Sayım uzman tarafından yapıldığında oldukça yorucu, uzun süren ve düşük doğruluğa sahip bir işlem haline gelmektedir. Bunun dışında hücre sayımını zorlaştrıan ve doğruluğu düşüren başka etkenler de mevcuttur. Bu nedenle, hücre sayımının otomatik bir şekilde yapılması ve sunulan sayım yöntemlerinin iyileştirilmesi gerekmektedir. Bu çalışmada, flüoresans mikroskop görüntüsünde otomatik hücre tespiti ve sayımı için bir yöntem sunulmuştur. Yöntemin tüm adımları açıklanmıştır. Sunulan yöntemin etkinliği birçok farklı durum için simülasyon programları vasıtasıyla test edilmiş, yöntemin başarıya ulaştığı ve gelecek vadeden bir çalışma olduğu gösterilmiştir.

Keywords

embriyonik kök hücre, otomatik kök hücre sayımı, histogram bölütleme, maksimum nokta analizi

An automatic embryonic stem cell counting method

Abstract

The cell counting process can be performed by manual counting in which a specialist counts the cells with naked eye or the automatic counting that utilizes the computer-based techniques. The counting process becomes exhausting, long and incorrect when the counting performed by specialist. Therefore the cell counting process must be performed automatically.In this study, an automatic cell detecting and counting method under fluorescence microscopy is proposed. All steps of the method are given in details. Several computer simulations are performed to evaluate the effectiveness of the method andit is shown that the proposed method gives promising results.   

Keywords

embriyonic stem cell, authomatic stem cell counting, histogram partitioning, maximum point analysis

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