Development and Analysis of Metabolic Syndrome Feeds for Laboratory Animals (Rats and Mice)

Yıl 2024, Sayı: 7, 15 - 23, 30.04.2024

Tayfun İde Aşkın Nur Derinöz Erdoğan Naim Deniz Ayaz

Öz

Feeding a high-fat diet causes obesity and metabolic disorders in rodents that resemble human metabolic syndrome. The aim of this study was the preparation and characterization of casein-based high-fat feeds to induce metabolic syndrome in laboratory animals (rats and mice). In this study, three different concentrations of high-fat feeds (24, 35 and 45%), which are stable, non-pollinating and shedding, were developed in pellet form. The parameters used in the characterization of feeds were established within the specific features required for these feeds to achieve their usage goals. To determine various components and properties of prepared feeds; In addition to physical, chemical and microbiological analyses, in-vivo nutritional tests were carried out by comparing them with imported commercial products developed for the same purposes. The results showed that the products obtained were in accordance with the production targets and were like the analysis values of the imported feeds compared. In conclusion, the study revealed that locally developed casein-based high-fat pellet feeds can be used reliably in studies of various companies and research centers to create obesity and metabolic disorders in experimental animals.

Anahtar Kelimeler

High-fat feed, obesity, metabolic syndrome diets, laboratory animals.

Proje Numarası

KOSGEB - Laboratuvar hayvanlarında (rat ve fare) obesite, diabet, metabolik bozukluk hastalıkları oluşturan kazein bazlı yüksek yağlı emülsiyon tipi yemlerin üretimi

Laboratuvar Hayvanları (Sıçanlar ve Fareler) İçin Metabolik Sendromlu Yemlerin Geliştirilmesi ve Analizi

Öz

Yüksek yağlı diyetle beslenme, kemirgenlerde insandaki metabolik sendroma benzeyen obeziteye ve metabolik bozukluklara neden olmaktadır. Bu çalışmanın amacı laboratuvar hayvanlarınde (sıçan ve fare) metabolic sendrom oluşturmak için kazein bazlı yüksek yağlı yemlerin hazırlanması ve karakterizasyonudur. Bu çalışmada, stabil, tozlaşmayan ve dökülme özelliğine sahip üç farklı konsantrasyonda yüksek yağlı yemler (%24, 35 ve %45) pelet formda geliştirilmiştir. Yemlerin karakterizasyonunda kullanılan parametreler bu yemlerin kullanım hedeflerine ulaşması için gerekli olan belirli özellikler dahilinde oluşturulmuştur. Hazırlanan yemlerde çeşitli bileşen ve özelliklerin belirlenmesi amacıyla; fiziksel, kimyasal, mikrobiyolojik analizlerin yanı sıra in-vivo beslenme testleri aynı amaçlarla geliştirilmiş ithal ticari ürünler ile karşılaştırılarak gerçekleştirilmiştir. Sonuçlar, elde edilen ürünlerin üretim hedeflerine uygun olarak elde edildiğini ve karşılaştırıldığı ithal yemlerin analiz değerlerine benzer olduğunu göstermiştir. Sonuç olarak çalışmada, yerli olarak geliştirilen kazein bazlı yüksek yağlı pelet yemlerin çeşitli firma ve araştırma merkezlerinin deney hayvanlarında obezite ve metabolik bozukluk oluşturmak çalışmalarında güvenilir bir şekilde kullanılabileceği ortaya konmuştur.

Anahtar Kelimeler

Yüksek yağlı yem, obezite, metabolik sendromlu diyetler, laboratuvar hayvanları.

Etik Beyan

In the study, experimental animal studies were performed in Kırıkkale University Hüseyin Aytemiz Experimental Animal Unit and Animal experiments were carried out with the approval of the Kırıkkale University Animal Experiments Local Ethics Committee (No: 2018-04-28).

Destekleyen Kurum

KOSGEB

Proje Numarası

KOSGEB - Laboratuvar hayvanlarında (rat ve fare) obesite, diabet, metabolik bozukluk hastalıkları oluşturan kazein bazlı yüksek yağlı emülsiyon tipi yemlerin üretimi

Teşekkür

This work was supported by KOSGEB project entitled “Production of casein-based high-fat emulsion type feeds that cause obesity, diabetes and metabolic disorders in laboratory animals (rats and mice). (Laboratuvar hayvanlarında (rat ve fare) obesite, diabet, metabolik bozukluk hastalıkları oluşturan kazein bazlı yüksek yağlı emülsiyon tipi yemlerin üretimi)”

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