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Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits

Yıl 2021, Cilt: 14 Sayı: 1, 11 - 17, 30.06.2021
https://doi.org/10.47027/duvetfd.841781

Öz

This study was conducted to investigate the effectiveness of amniotic fluid in defects in the epithelial tissue of the cornea. In the study, 14 New Zealand rabbits, which were experimentally created with a 3 mm diameter full-thickness defect in the corneal epithelium, were used. After the rabbits were randomly divided into two groups, amniotic fluid was applied to the rabbits in the first group (AF) and saline solution was applied to the rabbits in the second group (CG) three times a day for 20 days. Clinical examinations and tests were performed on the 7th, 14th, and 20th days of the study and the condition of the corneal defects was photographed. Clinical examinations revealed that tear break-up time (TBTT), fluorescein staining, rose bengal staining, corneal turbidity, and corneal vascularization were better in the AF group than in the CG group. As a result, it is concluded that amniotic fluid is effective in corneal epithelial defects and it would be beneficial to use it in addition to medical or operative treatment.

Destekleyen Kurum

FÜBAP

Proje Numarası

VF.18.03

Teşekkür

This project was supported by Fırat University Scientific Research Projects (FUBAP) with project number VF.18.03.

Kaynakça

  • 1. Suveren EH. (2010). Effect of human breast milk on corneal epithelial healing. Baskent University, Faculty of Medicine, Department of Eye Diseases. Master Thesis, Ankara.
  • 2. Gunay C, Saglıyan A, Yaman M. (2005). Effect of Acetylcystein on Treatment Outcome of Experimentally Induced Corneal Defects in Dogs. FÜ Sağ Bil Derg. 19 (2): 151-156.
  • 3. Kim KM, Shin YT, Kim HK. (2012). Effect of autologous platelet-rich plasma on persistent corneal epithelial defect after infectious keratitis. Jpn J Ophthalmol. 56: 544-550.
  • 4. Slatter D. (1990). Basıc Diagnostic Techniques. In: Fundamentals of Veterinary Ophthalmology, Ed: D. Slatter, W.B. Sounders Company. 84- 146.
  • 5. Torricelli AA, Santhanam A, Wu J, Singh V, Wilson SE. (2016). The corneal fibrosis response to epithelial-stromal injury. Exp Eye Res. 142: 110-118.
  • 6. Gunay C, Saglıyan A, Yılmaz S, Polat E, Kaya E, Han MC, Karabulut KK. (2020). Investigation of the Antioxidant Effects of Amniotic Fluid on Corneal Alkali Burns. FÜ Sağ Bil Vet Derg. 34(3): 165-171.
  • 7. Gilger BC, Whitley RD. (1991). Surgery of the Cornea and Sclera. In: Veterinary Ophthalmology, Ed. K.N. Gelatt, Oxford, UK: Blackwell Publishing Ltd, 675-700.
  • 8. Alexander VL, Mehrnoosh S. (2015). Progress in corneal wound healing. Prog Retin Eye Res. 49: 17-45.
  • 9. Crispin SM. (2005). Notes On Veterınary Ophthalmology. Section 1: Ophthalmic Equipment and Examination. Blackwell Science Ltd. a Blackwell Publishing Company, 1-20.
  • 10. Mould JRB. (2002). BSAVA Manual of Small Animal Ophthalmology, Ed: Simon Petersen-Jones and Sheila Crispin. Published by: British Small Animal Veterinary Association, 2 ed, 60-63.
  • 11. Alkan F, İzci C, Tepeli C, Koc Y. (2004). Evaluation of the schirmer tear test in twoTurkish breeds of shepherd dogs. Revue Med Vet. 155: 267-270.
  • 12. Sheila C. (2005). Notes On Veterinary Ophthalmology. Section 3: General and Canine Ophthalmology, Blackwell Publishing, 68-74.
  • 13. Akın F, Samsar E. (2005). Göz Hastalıkları, Medipres, Ankara.
  • 14. Adams GGW, Dilly PN, Kirkness CM. (1988). Monitoring Ocular Disease by Impression Cytology. Eye. 2: 506-516.
  • 15. Ofri R. (2008). Slatter’s Fundamentals of Veterinary Ophthalmology, Edition 4, Ed: Slatter JD. Saunders Elsevier. 285-286.
  • 16. Malkoç I. (2005). İnsan ve Görme Duyusu Gelişmiş Hayvanların Görme Yollarının Karşılaştırmalı Anatomisi, Doktora Tezi, Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü. Erzurum, 63.
  • 17. Sancak B, Cumhur M. (1999). Fonksiyonel Anatomi. Baş-Boyun ve İç Organlar. Metu Press, Ankara, 77-84.
  • 18. Hasıripi H, Recep OF. (2001). Pratik Göz Anatomisi. Işık Göz Kliniği Yayınları.
  • 19. DelMonte DW, Kim T. (2011). Anatomy and physiology of the cornea. J Cataract Refract Surg. 37: 588-598.
  • 20. Fujikawa LS, Foster CS, Harrist TJ, et al. (1981). Fibronectin in healing rabbit corneal wounds. Lab Invest. 45: 120-129.
  • 21. Anderson C, Zhou Q, Wang S. (2014). An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse. J Vis Exp. 86: 51-59.
  • 22. Bron AJ. (2001). Diagnosis of dry eye. Surv Ophthalmol. 45: 221-226.
  • 23. Savini G, Prabhawasat P, Kojima T, grueterich M, Espana E, Goto E. (2008). The challenge of dry eye diagnosis. Clin Ophthalmol. 2: 31-55.
  • 24. Barabino S, Chen W, Dana MR. (2004). Tear film and ocular surface tests in animal models of dry eye: uses and limitations. Exp Eye Res. 79: 613-621.
  • 25. Gunay C, Sağlıyan A, Özkaraca M, Han MC. (2013). Tavşanlarda deneysel oluşturulan kornea alkali yanıklarda otojen serumun kornea endotel iyileşmesi üzerine etkisi. FÜ Sağ Bil Vet Derg. 27: 31-34.
  • 26. Koç Y, Alkan F, Tepeli C. (2005). Schirmer tear test in different rabbit breeds. Hayvancılık Araştırma Dergisi. 15: 1-5.
  • 27. Kojima T, Ishida R, Dagru M, Goto E, Matsumato Y, Kaido M, Tsubota K. (2005). The effect of autologous serum eyedrops in the treatment of severe dry eye disease: A prospective randomized case-control study. Am J Ophthalmol. 139: 242-246.
  • 28. Gunay C, Saglıyan A, Yılmaz S, Kandemir FM, Han MC, Ozkarca M, Kulualp K. (2015). Evaluation of autologous serum eyedrops for the treatment of experimentally induced corneal alcali burns. Revue Méd Vét. 166: 63-71.
  • 29. Karagöz Y. (2015). SPSS 22 Uygulamalı Biyoistatistik. Güncellenmiş 2. Basım, Ankara: Nobel Tıp Kitabevi.
  • 30. Gelatt KN. (2014). Essentials of Veterinary Ophthalmology, Third Edition. Gelat KN, Wiley Blackwell, London.
  • 31. Wilkie DA, Whittaker C. (1997). Surgery of the cornea. Vet Clin North America Small Anim Prac. 27: 1067-1107.
  • 32. Gökçe P, Sarıtaş Z, Tong S. (2001). Köpeklerde deneysel penetran keratoplasti uygulamaları. Ankara Üniv Vet Fak Derg. 48: 67-73.
  • 33. Slatter D. (2001). Cornea and sclera. In: Fundamentals of Veterinary Ophthalmology, Ed: D Slatter, W.B. Saunders, Philadelphia, 293-313.
  • 34. Maggs DJ. (2008). Slatter’s Fundamentals of Veterinary Ophthalmology, Edition 4, Ed: Slatter JD. Saunders Elsevier. 135-150: 178-202.
  • 35. Samuelson DA. (1991). Ophthalmic Anatomy. In: Veterinary Ophthalmology. Ed: K.N. Gelatt, Oxford, UK: Blackwell Publishing Ltd., 45-150.
  • 36. Fox RI, Chan R, Michelson JB, Belmont JB, Michelson PE. (1984). Beneficial effect of artificial tears made with autologous serum in patients with keratoconjunctivitis sicca. Arthritis Rheum. 27: 459-461.
  • 37. Alio JL, Rodriguez AE, WrobelDudzińska D. (2015). Eye platelet-rich plasma in the treatment of ocular surface disorders. Curr Opin Ophthalmol. 26: 325-332.
  • 38. Pan Q, Angelina A, Zambrano A, Marrone M, Stark WJ, Heflin T, Tang L, Akpek EK. (2013). Autologous serum eye drops for dry eye. Cochrane Database Syst Rev, 8, CD009327.
  • 39. Saruhan BG, Akbalık ME, Topaloğlu U, Sağsöz H, Ketani MA, Altan S, Oğurtan Z. (2017). Tavşanlarda hidroflorik asit ile oluşturulan yanık sonrası, DMSO ve indometazinin korneal mast hücreleri üzerine etkilerinin araştırılması. Dicle Üniv Vet Fak Derg. 10: 130-137.
  • 40. Shahriari HA, Tokhmehchi F, Reza M, Hashemi NF. (2008). Comparison of the effect of amniotic membrane suspension and autologous serum on alkaline corneal epithelial wound healing in the rabbit model. Cornea. 27: 1148-1150.
  • 41. Sezen AY. (2017). Kornea Epitel İyileşmesinde Topikal İnsan Serumu, Amnion Sıvısı, Umbilikal Kord Serumu ve Anne Sütü Etkilerinin Karşılaştırılması. Uzmanlık Tezi, TC Başkent Üniversitesi Tıp Fakültesi Göz Hastalıkları Anabilim Dalı.
  • 42. Castro-Combs J, Noguera G, Cano M, Yew M, Gehlbach PL, Palmer J, Behrens A. (2008). Corneal wound healing is modulated by topical application of amniotic fluid in an ex vivo organ culture model. Exp Eye Res. 87: 56-63.
  • 43. Quinto GG, Castro-Combs J, Li L, Gupta N, Campos M, Behrens A. (2016). Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model. Int Ophthalmol. 36: 643-650.

Tavşanlarda deneysel kornea defektlerinin iyileşmesi üzerinde sığır amniyotik sıvısının etkinliğinin klinik değerlendirmesi

Yıl 2021, Cilt: 14 Sayı: 1, 11 - 17, 30.06.2021
https://doi.org/10.47027/duvetfd.841781

Öz

Bu çalışma, amniyotik sıvının kornea epitel dokusunda oluşan defektlerdeki etkinliğini araştırmak için yapıldı. Çalışmada, deneysel olarak kornea epitelinde 3 mm çapında tam kat defekt oluşturulmuş 14 adet Yeni Zelanda tavşanı kullanıldı. Tavşanlar rastgele iki gruba ayrıldıktan sonra birinci gruptaki tavşanlara (AF) amniyon sıvısı, ikinci gruptaki tavşanlara (CG) fizyolojik tuzlu su 20 gün süreyle günde üç kez uygulandı.Çalışmanın 7., 14. ve 20. günlerinde klinik muayene ve testler yapılarak kornea defektlerinin durumu fotoğraflandı. Klinik incelemeler sonucunda AF grubundaki deneklerde gözyaşı kırılma zamanı (TBTT), floresein boyama, rose bengal boyama, korneal bulanıklık ve korneal vaskülarizasyon durumunun CG grubuna göre daha iyi olduğunu ortaya konuldu. Sonuç olarak amniyon sıvısının kornea epitel defektlerinde etkili olduğu, medikal veya operatif tedavilere ek olarak kullanılmasının faydalı olacağı sonucuna varıldı.

Proje Numarası

VF.18.03

Kaynakça

  • 1. Suveren EH. (2010). Effect of human breast milk on corneal epithelial healing. Baskent University, Faculty of Medicine, Department of Eye Diseases. Master Thesis, Ankara.
  • 2. Gunay C, Saglıyan A, Yaman M. (2005). Effect of Acetylcystein on Treatment Outcome of Experimentally Induced Corneal Defects in Dogs. FÜ Sağ Bil Derg. 19 (2): 151-156.
  • 3. Kim KM, Shin YT, Kim HK. (2012). Effect of autologous platelet-rich plasma on persistent corneal epithelial defect after infectious keratitis. Jpn J Ophthalmol. 56: 544-550.
  • 4. Slatter D. (1990). Basıc Diagnostic Techniques. In: Fundamentals of Veterinary Ophthalmology, Ed: D. Slatter, W.B. Sounders Company. 84- 146.
  • 5. Torricelli AA, Santhanam A, Wu J, Singh V, Wilson SE. (2016). The corneal fibrosis response to epithelial-stromal injury. Exp Eye Res. 142: 110-118.
  • 6. Gunay C, Saglıyan A, Yılmaz S, Polat E, Kaya E, Han MC, Karabulut KK. (2020). Investigation of the Antioxidant Effects of Amniotic Fluid on Corneal Alkali Burns. FÜ Sağ Bil Vet Derg. 34(3): 165-171.
  • 7. Gilger BC, Whitley RD. (1991). Surgery of the Cornea and Sclera. In: Veterinary Ophthalmology, Ed. K.N. Gelatt, Oxford, UK: Blackwell Publishing Ltd, 675-700.
  • 8. Alexander VL, Mehrnoosh S. (2015). Progress in corneal wound healing. Prog Retin Eye Res. 49: 17-45.
  • 9. Crispin SM. (2005). Notes On Veterınary Ophthalmology. Section 1: Ophthalmic Equipment and Examination. Blackwell Science Ltd. a Blackwell Publishing Company, 1-20.
  • 10. Mould JRB. (2002). BSAVA Manual of Small Animal Ophthalmology, Ed: Simon Petersen-Jones and Sheila Crispin. Published by: British Small Animal Veterinary Association, 2 ed, 60-63.
  • 11. Alkan F, İzci C, Tepeli C, Koc Y. (2004). Evaluation of the schirmer tear test in twoTurkish breeds of shepherd dogs. Revue Med Vet. 155: 267-270.
  • 12. Sheila C. (2005). Notes On Veterinary Ophthalmology. Section 3: General and Canine Ophthalmology, Blackwell Publishing, 68-74.
  • 13. Akın F, Samsar E. (2005). Göz Hastalıkları, Medipres, Ankara.
  • 14. Adams GGW, Dilly PN, Kirkness CM. (1988). Monitoring Ocular Disease by Impression Cytology. Eye. 2: 506-516.
  • 15. Ofri R. (2008). Slatter’s Fundamentals of Veterinary Ophthalmology, Edition 4, Ed: Slatter JD. Saunders Elsevier. 285-286.
  • 16. Malkoç I. (2005). İnsan ve Görme Duyusu Gelişmiş Hayvanların Görme Yollarının Karşılaştırmalı Anatomisi, Doktora Tezi, Atatürk Üniversitesi Sağlık Bilimleri Enstitüsü. Erzurum, 63.
  • 17. Sancak B, Cumhur M. (1999). Fonksiyonel Anatomi. Baş-Boyun ve İç Organlar. Metu Press, Ankara, 77-84.
  • 18. Hasıripi H, Recep OF. (2001). Pratik Göz Anatomisi. Işık Göz Kliniği Yayınları.
  • 19. DelMonte DW, Kim T. (2011). Anatomy and physiology of the cornea. J Cataract Refract Surg. 37: 588-598.
  • 20. Fujikawa LS, Foster CS, Harrist TJ, et al. (1981). Fibronectin in healing rabbit corneal wounds. Lab Invest. 45: 120-129.
  • 21. Anderson C, Zhou Q, Wang S. (2014). An Alkali-burn Injury Model of Corneal Neovascularization in the Mouse. J Vis Exp. 86: 51-59.
  • 22. Bron AJ. (2001). Diagnosis of dry eye. Surv Ophthalmol. 45: 221-226.
  • 23. Savini G, Prabhawasat P, Kojima T, grueterich M, Espana E, Goto E. (2008). The challenge of dry eye diagnosis. Clin Ophthalmol. 2: 31-55.
  • 24. Barabino S, Chen W, Dana MR. (2004). Tear film and ocular surface tests in animal models of dry eye: uses and limitations. Exp Eye Res. 79: 613-621.
  • 25. Gunay C, Sağlıyan A, Özkaraca M, Han MC. (2013). Tavşanlarda deneysel oluşturulan kornea alkali yanıklarda otojen serumun kornea endotel iyileşmesi üzerine etkisi. FÜ Sağ Bil Vet Derg. 27: 31-34.
  • 26. Koç Y, Alkan F, Tepeli C. (2005). Schirmer tear test in different rabbit breeds. Hayvancılık Araştırma Dergisi. 15: 1-5.
  • 27. Kojima T, Ishida R, Dagru M, Goto E, Matsumato Y, Kaido M, Tsubota K. (2005). The effect of autologous serum eyedrops in the treatment of severe dry eye disease: A prospective randomized case-control study. Am J Ophthalmol. 139: 242-246.
  • 28. Gunay C, Saglıyan A, Yılmaz S, Kandemir FM, Han MC, Ozkarca M, Kulualp K. (2015). Evaluation of autologous serum eyedrops for the treatment of experimentally induced corneal alcali burns. Revue Méd Vét. 166: 63-71.
  • 29. Karagöz Y. (2015). SPSS 22 Uygulamalı Biyoistatistik. Güncellenmiş 2. Basım, Ankara: Nobel Tıp Kitabevi.
  • 30. Gelatt KN. (2014). Essentials of Veterinary Ophthalmology, Third Edition. Gelat KN, Wiley Blackwell, London.
  • 31. Wilkie DA, Whittaker C. (1997). Surgery of the cornea. Vet Clin North America Small Anim Prac. 27: 1067-1107.
  • 32. Gökçe P, Sarıtaş Z, Tong S. (2001). Köpeklerde deneysel penetran keratoplasti uygulamaları. Ankara Üniv Vet Fak Derg. 48: 67-73.
  • 33. Slatter D. (2001). Cornea and sclera. In: Fundamentals of Veterinary Ophthalmology, Ed: D Slatter, W.B. Saunders, Philadelphia, 293-313.
  • 34. Maggs DJ. (2008). Slatter’s Fundamentals of Veterinary Ophthalmology, Edition 4, Ed: Slatter JD. Saunders Elsevier. 135-150: 178-202.
  • 35. Samuelson DA. (1991). Ophthalmic Anatomy. In: Veterinary Ophthalmology. Ed: K.N. Gelatt, Oxford, UK: Blackwell Publishing Ltd., 45-150.
  • 36. Fox RI, Chan R, Michelson JB, Belmont JB, Michelson PE. (1984). Beneficial effect of artificial tears made with autologous serum in patients with keratoconjunctivitis sicca. Arthritis Rheum. 27: 459-461.
  • 37. Alio JL, Rodriguez AE, WrobelDudzińska D. (2015). Eye platelet-rich plasma in the treatment of ocular surface disorders. Curr Opin Ophthalmol. 26: 325-332.
  • 38. Pan Q, Angelina A, Zambrano A, Marrone M, Stark WJ, Heflin T, Tang L, Akpek EK. (2013). Autologous serum eye drops for dry eye. Cochrane Database Syst Rev, 8, CD009327.
  • 39. Saruhan BG, Akbalık ME, Topaloğlu U, Sağsöz H, Ketani MA, Altan S, Oğurtan Z. (2017). Tavşanlarda hidroflorik asit ile oluşturulan yanık sonrası, DMSO ve indometazinin korneal mast hücreleri üzerine etkilerinin araştırılması. Dicle Üniv Vet Fak Derg. 10: 130-137.
  • 40. Shahriari HA, Tokhmehchi F, Reza M, Hashemi NF. (2008). Comparison of the effect of amniotic membrane suspension and autologous serum on alkaline corneal epithelial wound healing in the rabbit model. Cornea. 27: 1148-1150.
  • 41. Sezen AY. (2017). Kornea Epitel İyileşmesinde Topikal İnsan Serumu, Amnion Sıvısı, Umbilikal Kord Serumu ve Anne Sütü Etkilerinin Karşılaştırılması. Uzmanlık Tezi, TC Başkent Üniversitesi Tıp Fakültesi Göz Hastalıkları Anabilim Dalı.
  • 42. Castro-Combs J, Noguera G, Cano M, Yew M, Gehlbach PL, Palmer J, Behrens A. (2008). Corneal wound healing is modulated by topical application of amniotic fluid in an ex vivo organ culture model. Exp Eye Res. 87: 56-63.
  • 43. Quinto GG, Castro-Combs J, Li L, Gupta N, Campos M, Behrens A. (2016). Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model. Int Ophthalmol. 36: 643-650.
Toplam 43 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Araştıma
Yazarlar

Aydın Sağlıyan 0000-0002-8226-0740

Merve Şahin 0000-0001-9517-0579

Cihan Günay 0000-0002-0476-6164

Ali Said Durmuş 0000-0003-4873-5690

Eren Polat 0000-0002-3999-1310

Proje Numarası VF.18.03
Yayımlanma Tarihi 30 Haziran 2021
Kabul Tarihi 28 Şubat 2021
Yayımlandığı Sayı Yıl 2021 Cilt: 14 Sayı: 1

Kaynak Göster

APA Sağlıyan, A., Şahin, M., Günay, C., Durmuş, A. S., vd. (2021). Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 14(1), 11-17. https://doi.org/10.47027/duvetfd.841781
AMA Sağlıyan A, Şahin M, Günay C, Durmuş AS, Polat E. Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits. Dicle Üniv Vet Fak Derg. Haziran 2021;14(1):11-17. doi:10.47027/duvetfd.841781
Chicago Sağlıyan, Aydın, Merve Şahin, Cihan Günay, Ali Said Durmuş, ve Eren Polat. “Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14, sy. 1 (Haziran 2021): 11-17. https://doi.org/10.47027/duvetfd.841781.
EndNote Sağlıyan A, Şahin M, Günay C, Durmuş AS, Polat E (01 Haziran 2021) Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14 1 11–17.
IEEE A. Sağlıyan, M. Şahin, C. Günay, A. S. Durmuş, ve E. Polat, “Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits”, Dicle Üniv Vet Fak Derg, c. 14, sy. 1, ss. 11–17, 2021, doi: 10.47027/duvetfd.841781.
ISNAD Sağlıyan, Aydın vd. “Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 14/1 (Haziran 2021), 11-17. https://doi.org/10.47027/duvetfd.841781.
JAMA Sağlıyan A, Şahin M, Günay C, Durmuş AS, Polat E. Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits. Dicle Üniv Vet Fak Derg. 2021;14:11–17.
MLA Sağlıyan, Aydın vd. “Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, c. 14, sy. 1, 2021, ss. 11-17, doi:10.47027/duvetfd.841781.
Vancouver Sağlıyan A, Şahin M, Günay C, Durmuş AS, Polat E. Clinical Evaluation of the Efficacy of Bovine Amniotic Fluid on Healing of Experimental Corneal Defects in Rabbits. Dicle Üniv Vet Fak Derg. 2021;14(1):11-7.