Lactobacillus Plantarum LP299v Suşu Hücre İçermeyen Süpernatanının RAW 264.7 Makrofaj Hücrelerinde İmmünmodülatör Aktivitelerinin Analizleri

Year 2023, , 1356 - 1362, 28.12.2023

Tugce Karaduman

https://doi.org/10.35414/akufemubid.1302005

Abstract

İnsan bağışıklık sistemi, vücudu, zararlı iç ve dış unsurlara karşı savunmak için gereklidir; bağışıklık, doğuştan gelen ve edinilmiş bağışıklığı içerir. Doğuştan gelen bağışıklık sisteminin temel bileşeni olan makrofajlar, ölü hücrelerin ve tümör hücrelerinin yanı sıra fagositozu tetikleyerek yabancı maddelerin temizlenmesi için çok önemlidir. Ayrıca salgıladıkları sitokinler ve aracı moleküller ile adaptif yanıtta da rol oynarlar. Probiyotiklerin önemli bir sınıfı olan laktik asit bakterileri (LAB), konakçı sağlığını iyileştirmek ve fonksiyonel bir gıda olarak kullanılmak için güçlü bir potansiyele sahiptir. Bazı LAB suşlarının bağışıklık sistemini uyarıcı etkileri olduğu bildirilmiştir. Bununla birlikte, bazı LAB suşlarından elde edilen hücresiz süpernatantların (CFS) makrofaj aktivasyonu üzerindeki etkileri son yıllarda önemli bir araştırma alanı haline gelmiştir. Bu çalışmanın temel amacı, Lactobacillus plantarum LP299v'nin RAW 264.7 makrofaj hücre hattındaki immünostimülatör aktivitelerini karakterize etmektir. Bu amaçla, ilgili suşun CFS'sinin immünomodülatör aktivitesi, makrofaj hücre hattı kullanılarak proliferasyon, fagositoz yeteneği ve nitrik oksit (NO) üretimi parametreleri açısından sırasıyla MTT, nötral kırmızı testi ve Griess reaksiyonu ile değerlendirilmiştir. Çalışmalar, bu suşun RAW264.7 makrofaj hücrelerinde proliferasyon, fagositoz ve NO seviyelerini önemli ölçüde artırdığını göstermiştir. Bu sonuçlar birlikte ele alındığında, bu çalışmada seçilen Lactobacillus plantarum LP299v'den elde edilen hücresiz süpernatanın immünostimülatör aktiviteye sahip aday bileşikler için yararlı olabileceğini göstermektedir.

Keywords

Lactobacillus, Hücresiz süpernatan, RAW 264.7, İmmunomodülatör aktivite

Project Number

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Immunomodulatory Activity Analyses of Cell-Free Supernatant of Lactobacillus plantarum LP299v Strain in RAW 264.7 Macrophage Cells

Abstract

The human immune system is essential for defending the body against harmful internal and external elements; immunity includes innate and acquired immunity. Macrophages, the innate immune system's key components, are crucial for the clearance of dead cells and tumor cells, as well as foreign substances by triggering phagocytosis. They also play a role in the adaptive response with the cytokines and mediator molecules they secrete. Lactic acid bacteria (LAB), an important probiotics class, have a strong potential to improve host health and can be used as a functional food. There have been reports of certain LAB strains having immunostimulating effects. However, the effects of cell-free supernatants (CFS) gathered from some LAB strains on macrophage activation have become an important research area in recent years. This study's main objective was to characterize the immunostimulatory activities of Lactobacillus plantarum LP299v in the RAW 264.7 macrophage cell line. For this purpose, the immunomodulatory activity of CFS of the related strain was evaluated by MTT, neutral red assay, and Griess reaction respectively, in terms of proliferation, phagocytosis ability, and nitric oxide (NO) production parameters using the macrophage cell line. Studies have shown that this strain significantly increased proliferation, phagocytosis, and NO levels in RAW264.7 macrophage cells. When considered, these results suggest that the cell-free supernatant, obtained from Lactobacillus plantarum LP299v selected in this study, may be helpful for candidate compounds with immunostimulatory activity.

Keywords

Lactobacillus, Cell-free supernatant, RAW 264.7, Immunomodulatory activity

Supporting Institution

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Project Number

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Thanks

We thank Aksaray University Scientific and Technological Application and Research Center (ASÜBTAM) for their support in this study and Dokuz Eylül University Oncology Institute for the supply of RAW 264.7 cells.

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